Experiment: LB, pH4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS33570 and BPHYT_RS33575 are separated by 254 nucleotides BPHYT_RS33575 and BPHYT_RS33580 are separated by 85 nucleotides BPHYT_RS33580 and BPHYT_RS33585 overlap by 4 nucleotides
BPHYT_RS33570: BPHYT_RS33570 - GTP cyclohydrolase, at 3,115,760 to 3,116,566
_RS33570
BPHYT_RS33575: BPHYT_RS33575 - 1-deoxy-D-xylulose-5-phosphate synthase, at 3,116,821 to 3,118,728
_RS33575
BPHYT_RS33580: BPHYT_RS33580 - geranyltranstransferase, at 3,118,814 to 3,119,695
_RS33580
BPHYT_RS33585: BPHYT_RS33585 - exodeoxyribonuclease VII small subunit, at 3,119,692 to 3,120,000
_RS33585
Position (kb)
3116
3117
3118
3119 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3115.843 kb on - strand, within BPHYT_RS33570 at 3115.843 kb on - strand, within BPHYT_RS33570 at 3116.069 kb on - strand, within BPHYT_RS33570 at 3116.079 kb on + strand, within BPHYT_RS33570 at 3116.779 kb on - strand at 3116.779 kb on - strand at 3118.743 kb on - strand at 3118.770 kb on - strand at 3118.770 kb on - strand at 3118.770 kb on - strand at 3118.770 kb on - strand at 3118.770 kb on - strand at 3118.770 kb on - strand at 3118.783 kb on - strand at 3118.957 kb on - strand, within BPHYT_RS33580 at 3118.957 kb on - strand, within BPHYT_RS33580 at 3119.074 kb on - strand, within BPHYT_RS33580 at 3119.383 kb on - strand, within BPHYT_RS33580 at 3119.394 kb on - strand at 3119.394 kb on - strand, within BPHYT_RS33580 at 3119.461 kb on - strand, within BPHYT_RS33580 at 3119.461 kb on - strand, within BPHYT_RS33580 at 3119.461 kb on - strand, within BPHYT_RS33580 at 3119.635 kb on - strand at 3119.696 kb on - strand at 3119.696 kb on - strand at 3119.696 kb on - strand at 3119.696 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH4 remove 3,115,843 - BPHYT_RS33570 0.10 -0.1 3,115,843 - BPHYT_RS33570 0.10 -0.3 3,116,069 - BPHYT_RS33570 0.38 -0.3 3,116,079 + BPHYT_RS33570 0.40 +0.0 3,116,779 - -0.3 3,116,779 - -0.0 3,118,743 - +0.3 3,118,770 - +0.2 3,118,770 - -0.1 3,118,770 - -0.2 3,118,770 - +0.1 3,118,770 - +0.3 3,118,770 - -0.1 3,118,783 - -0.7 3,118,957 - BPHYT_RS33580 0.16 -4.2 3,118,957 - BPHYT_RS33580 0.16 -3.9 3,119,074 - BPHYT_RS33580 0.29 -4.0 3,119,383 - BPHYT_RS33580 0.65 -1.6 3,119,394 - -0.3 3,119,394 - BPHYT_RS33580 0.66 -5.7 3,119,461 - BPHYT_RS33580 0.73 -4.5 3,119,461 - BPHYT_RS33580 0.73 -1.3 3,119,461 - BPHYT_RS33580 0.73 -2.8 3,119,635 - -2.5 3,119,696 - -0.4 3,119,696 - +0.4 3,119,696 - +0.3 3,119,696 - +0.9
Or see this region's nucleotide sequence