Experiment: LB, pH4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22260 and BPHYT_RS22265 are separated by 106 nucleotides BPHYT_RS22265 and BPHYT_RS22270 are separated by 10 nucleotides BPHYT_RS22270 and BPHYT_RS22275 overlap by 4 nucleotides BPHYT_RS22275 and BPHYT_RS22280 overlap by 8 nucleotides
BPHYT_RS22260: BPHYT_RS22260 - hypothetical protein, at 551,318 to 551,608
_RS22260
BPHYT_RS22265: BPHYT_RS22265 - hypothetical protein, at 551,715 to 552,506
_RS22265
BPHYT_RS22270: BPHYT_RS22270 - membrane protein, at 552,517 to 555,135
_RS22270
BPHYT_RS22275: BPHYT_RS22275 - membrane protein, at 555,132 to 555,779
_RS22275
BPHYT_RS22280: BPHYT_RS22280 - hypothetical protein, at 555,772 to 556,425
_RS22280
Position (kb) 552
553
554
555
556 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 551.606 kb on + strand at 551.607 kb on - strand at 551.623 kb on + strand at 551.715 kb on - strand at 551.768 kb on - strand at 551.768 kb on - strand at 551.940 kb on + strand, within BPHYT_RS22265 at 551.976 kb on - strand, within BPHYT_RS22265 at 552.516 kb on + strand at 552.516 kb on + strand at 552.517 kb on - strand at 552.517 kb on - strand at 552.526 kb on + strand at 552.530 kb on + strand at 552.643 kb on + strand at 552.643 kb on + strand at 552.644 kb on - strand at 552.673 kb on + strand at 553.120 kb on + strand, within BPHYT_RS22270 at 553.300 kb on + strand, within BPHYT_RS22270 at 553.302 kb on + strand, within BPHYT_RS22270 at 553.303 kb on - strand, within BPHYT_RS22270 at 553.808 kb on + strand, within BPHYT_RS22270 at 554.287 kb on - strand, within BPHYT_RS22270 at 554.287 kb on - strand, within BPHYT_RS22270 at 554.964 kb on + strand at 554.965 kb on - strand at 555.016 kb on + strand at 555.016 kb on + strand at 555.524 kb on + strand, within BPHYT_RS22275 at 555.730 kb on + strand at 555.730 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH4 remove 551,606 + -0.7 551,607 - +0.3 551,623 + -0.1 551,715 - +0.0 551,768 - +0.4 551,768 - -0.2 551,940 + BPHYT_RS22265 0.28 +0.1 551,976 - BPHYT_RS22265 0.33 -3.0 552,516 + +0.4 552,516 + +0.6 552,517 - +0.0 552,517 - +0.0 552,526 + -0.6 552,530 + +0.6 552,643 + +0.7 552,643 + -0.6 552,644 - +0.0 552,673 + -0.8 553,120 + BPHYT_RS22270 0.23 +0.5 553,300 + BPHYT_RS22270 0.30 -0.0 553,302 + BPHYT_RS22270 0.30 +1.8 553,303 - BPHYT_RS22270 0.30 +0.4 553,808 + BPHYT_RS22270 0.49 +1.0 554,287 - BPHYT_RS22270 0.68 +1.1 554,287 - BPHYT_RS22270 0.68 +0.3 554,964 + -0.0 554,965 - +0.3 555,016 + +0.0 555,016 + +0.3 555,524 + BPHYT_RS22275 0.60 -0.8 555,730 + +0.2 555,730 + -0.6
Or see this region's nucleotide sequence