Experiment: LB, pH4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12985 and BPHYT_RS12990 are separated by 63 nucleotides BPHYT_RS12990 and BPHYT_RS12995 are separated by 741 nucleotides
BPHYT_RS12985: BPHYT_RS12985 - amino acid acetyltransferase, at 2,969,554 to 2,970,942
_RS12985
BPHYT_RS12990: BPHYT_RS12990 - iron transporter, at 2,971,006 to 2,971,272
_RS12990
BPHYT_RS12995: BPHYT_RS12995 - MFS transporter, at 2,972,014 to 2,973,483
_RS12995
Position (kb)
2971
2972 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2970.316 kb on + strand, within BPHYT_RS12985 at 2970.487 kb on + strand, within BPHYT_RS12985 at 2970.899 kb on - strand at 2970.950 kb on + strand at 2970.951 kb on - strand at 2970.951 kb on - strand at 2970.951 kb on - strand at 2970.951 kb on - strand at 2970.951 kb on - strand at 2970.954 kb on + strand at 2970.954 kb on + strand at 2970.955 kb on - strand at 2970.955 kb on - strand at 2970.955 kb on - strand at 2971.005 kb on + strand at 2971.005 kb on + strand at 2971.087 kb on + strand, within BPHYT_RS12990 at 2971.088 kb on - strand, within BPHYT_RS12990 at 2971.249 kb on + strand at 2971.249 kb on + strand at 2971.270 kb on + strand at 2971.270 kb on + strand at 2971.275 kb on + strand at 2971.275 kb on + strand at 2971.275 kb on + strand at 2971.276 kb on - strand at 2971.293 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH4 remove 2,970,316 + BPHYT_RS12985 0.55 +0.4 2,970,487 + BPHYT_RS12985 0.67 +0.6 2,970,899 - -0.3 2,970,950 + -0.5 2,970,951 - -0.2 2,970,951 - -0.4 2,970,951 - -0.1 2,970,951 - -0.3 2,970,951 - +0.1 2,970,954 + -0.4 2,970,954 + +0.5 2,970,955 - -0.7 2,970,955 - -1.3 2,970,955 - +0.3 2,971,005 + -0.5 2,971,005 + +0.5 2,971,087 + BPHYT_RS12990 0.30 -4.1 2,971,088 - BPHYT_RS12990 0.31 -1.8 2,971,249 + +0.1 2,971,249 + -0.3 2,971,270 + -0.3 2,971,270 + +0.2 2,971,275 + -0.5 2,971,275 + +0.8 2,971,275 + -0.3 2,971,276 - +0.0 2,971,293 + -0.3
Or see this region's nucleotide sequence