Experiment: LB, pH4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS07995 and BPHYT_RS08000 are separated by 553 nucleotides BPHYT_RS08000 and BPHYT_RS08005 are separated by 83 nucleotides BPHYT_RS08005 and BPHYT_RS08010 are separated by 256 nucleotides
BPHYT_RS07995: BPHYT_RS07995 - diguanylate phosphodiesterase, at 1,825,063 to 1,826,316
_RS07995
BPHYT_RS08000: BPHYT_RS08000 - antibiotic biosynthesis monooxygenase, at 1,826,870 to 1,827,160
_RS08000
BPHYT_RS08005: BPHYT_RS08005 - DNA-binding protein, at 1,827,244 to 1,827,501
_RS08005
BPHYT_RS08010: BPHYT_RS08010 - hypothetical protein, at 1,827,758 to 1,827,997
_RS08010
Position (kb)
1826
1827
1828 Strain fitness (log2 ratio)
-2
-1
0
1 at 1825.892 kb on - strand, within BPHYT_RS07995 at 1826.168 kb on + strand, within BPHYT_RS07995 at 1826.218 kb on - strand at 1826.218 kb on - strand at 1826.316 kb on + strand at 1826.432 kb on - strand at 1826.499 kb on - strand at 1826.530 kb on + strand at 1826.681 kb on + strand at 1826.987 kb on + strand, within BPHYT_RS08000 at 1826.987 kb on + strand, within BPHYT_RS08000 at 1827.098 kb on - strand, within BPHYT_RS08000 at 1827.175 kb on + strand at 1827.185 kb on + strand at 1827.672 kb on + strand at 1827.673 kb on - strand at 1827.673 kb on - strand at 1828.001 kb on + strand at 1828.002 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH4 remove 1,825,892 - BPHYT_RS07995 0.66 -0.3 1,826,168 + BPHYT_RS07995 0.88 -0.3 1,826,218 - -0.1 1,826,218 - -0.8 1,826,316 + +0.2 1,826,432 - -2.0 1,826,499 - +0.8 1,826,530 + +0.5 1,826,681 + -0.0 1,826,987 + BPHYT_RS08000 0.40 -0.0 1,826,987 + BPHYT_RS08000 0.40 +1.2 1,827,098 - BPHYT_RS08000 0.78 +0.8 1,827,175 + +0.5 1,827,185 + +0.4 1,827,672 + -0.4 1,827,673 - -0.6 1,827,673 - -0.5 1,828,001 + -0.2 1,828,002 - -0.2
Or see this region's nucleotide sequence