Experiment: D-Salicin (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS14485 and CA265_RS14490 are separated by 173 nucleotides CA265_RS14490 and CA265_RS14495 are separated by 38 nucleotides CA265_RS14495 and CA265_RS14500 are separated by 255 nucleotides CA265_RS14500 and CA265_RS14505 overlap by 7 nucleotides CA265_RS14505 and CA265_RS14510 are separated by 78 nucleotides
CA265_RS14485: CA265_RS14485 - bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase, at 3,651,728 to 3,653,935
_RS14485
CA265_RS14490: CA265_RS14490 - transcriptional repressor, at 3,654,109 to 3,654,606
_RS14490
CA265_RS14495: CA265_RS14495 - adenylosuccinate synthase, at 3,654,645 to 3,655,925
_RS14495
CA265_RS14500: CA265_RS14500 - hypothetical protein, at 3,656,181 to 3,656,408
_RS14500
CA265_RS14505: CA265_RS14505 - hypothetical protein, at 3,656,402 to 3,656,698
_RS14505
CA265_RS14510: CA265_RS14510 - hypothetical protein, at 3,656,777 to 3,657,220
_RS14510
Position (kb)
3654
3655
3656 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3653.934 kb on - strand at 3653.937 kb on + strand at 3653.972 kb on - strand at 3654.035 kb on + strand at 3654.035 kb on + strand at 3654.080 kb on + strand at 3654.084 kb on + strand at 3654.614 kb on + strand at 3654.614 kb on + strand at 3655.926 kb on + strand at 3655.927 kb on - strand at 3655.965 kb on - strand at 3655.983 kb on + strand at 3655.984 kb on - strand at 3655.996 kb on - strand at 3656.076 kb on - strand at 3656.126 kb on - strand at 3656.168 kb on - strand at 3656.177 kb on + strand at 3656.190 kb on - strand at 3656.211 kb on - strand, within CA265_RS14500 at 3656.252 kb on - strand, within CA265_RS14500 at 3656.254 kb on + strand, within CA265_RS14500 at 3656.255 kb on - strand, within CA265_RS14500 at 3656.265 kb on - strand, within CA265_RS14500 at 3656.302 kb on + strand, within CA265_RS14500 at 3656.318 kb on + strand, within CA265_RS14500 at 3656.360 kb on + strand, within CA265_RS14500 at 3656.360 kb on + strand, within CA265_RS14500 at 3656.364 kb on + strand, within CA265_RS14500 at 3656.365 kb on - strand, within CA265_RS14500 at 3656.366 kb on + strand, within CA265_RS14500 at 3656.387 kb on - strand at 3656.464 kb on - strand at 3656.466 kb on - strand, within CA265_RS14505 at 3656.467 kb on + strand, within CA265_RS14505 at 3656.468 kb on - strand, within CA265_RS14505 at 3656.468 kb on - strand, within CA265_RS14505 at 3656.471 kb on + strand, within CA265_RS14505 at 3656.472 kb on - strand, within CA265_RS14505 at 3656.479 kb on + strand, within CA265_RS14505 at 3656.488 kb on + strand, within CA265_RS14505 at 3656.512 kb on + strand, within CA265_RS14505 at 3656.539 kb on + strand, within CA265_RS14505 at 3656.577 kb on - strand, within CA265_RS14505 at 3656.596 kb on + strand, within CA265_RS14505 at 3656.597 kb on - strand, within CA265_RS14505 at 3656.597 kb on - strand, within CA265_RS14505 at 3656.601 kb on - strand, within CA265_RS14505 at 3656.601 kb on - strand, within CA265_RS14505 at 3656.602 kb on - strand, within CA265_RS14505 at 3656.665 kb on - strand, within CA265_RS14505 at 3656.668 kb on - strand, within CA265_RS14505 at 3656.674 kb on + strand at 3656.696 kb on + strand at 3656.726 kb on + strand at 3656.910 kb on + strand, within CA265_RS14510
Per-strain Table
Position Strand Gene LocusTag Fraction D-Salicin (C) remove 3,653,934 - -0.1 3,653,937 + -0.6 3,653,972 - +0.4 3,654,035 + -0.3 3,654,035 + +0.6 3,654,080 + -0.5 3,654,084 + +0.1 3,654,614 + +0.9 3,654,614 + +2.0 3,655,926 + -1.5 3,655,927 - +0.1 3,655,965 - +1.6 3,655,983 + -0.2 3,655,984 - +1.7 3,655,996 - -0.4 3,656,076 - +0.1 3,656,126 - -0.2 3,656,168 - +0.9 3,656,177 + +2.0 3,656,190 - -0.3 3,656,211 - CA265_RS14500 0.13 -0.5 3,656,252 - CA265_RS14500 0.31 -2.4 3,656,254 + CA265_RS14500 0.32 +0.3 3,656,255 - CA265_RS14500 0.32 +0.0 3,656,265 - CA265_RS14500 0.37 -2.4 3,656,302 + CA265_RS14500 0.53 +0.1 3,656,318 + CA265_RS14500 0.60 +0.3 3,656,360 + CA265_RS14500 0.79 -1.4 3,656,360 + CA265_RS14500 0.79 -0.0 3,656,364 + CA265_RS14500 0.80 -0.5 3,656,365 - CA265_RS14500 0.81 -0.1 3,656,366 + CA265_RS14500 0.81 +0.2 3,656,387 - -0.8 3,656,464 - +1.6 3,656,466 - CA265_RS14505 0.22 -0.5 3,656,467 + CA265_RS14505 0.22 +0.6 3,656,468 - CA265_RS14505 0.22 -1.2 3,656,468 - CA265_RS14505 0.22 +0.3 3,656,471 + CA265_RS14505 0.23 +1.0 3,656,472 - CA265_RS14505 0.24 +0.3 3,656,479 + CA265_RS14505 0.26 +0.5 3,656,488 + CA265_RS14505 0.29 +0.9 3,656,512 + CA265_RS14505 0.37 +0.1 3,656,539 + CA265_RS14505 0.46 +0.5 3,656,577 - CA265_RS14505 0.59 +1.0 3,656,596 + CA265_RS14505 0.65 +0.2 3,656,597 - CA265_RS14505 0.66 -0.1 3,656,597 - CA265_RS14505 0.66 +1.3 3,656,601 - CA265_RS14505 0.67 -0.2 3,656,601 - CA265_RS14505 0.67 +1.4 3,656,602 - CA265_RS14505 0.67 +0.5 3,656,665 - CA265_RS14505 0.89 +0.8 3,656,668 - CA265_RS14505 0.90 +0.4 3,656,674 + +0.3 3,656,696 + +0.5 3,656,726 + +0.2 3,656,910 + CA265_RS14510 0.30 -0.0
Or see this region's nucleotide sequence