Experiment: Fusidic 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc03847 and SMc03848 overlap by 4 nucleotides SMc03848 and SMc03849 are separated by 59 nucleotides SMc03849 and SMc03850 overlap by 4 nucleotides SMc03850 and SMc03851 overlap by 4 nucleotides SMc03851 and SMc03852 are separated by 158 nucleotides
SMc03847: SMc03847 - cytochrome c biogenesis protein CcmA, at 3,513,863 to 3,514,486
SMc03847
SMc03848: SMc03848 - heme exporter B (cytochrome C-type biogenesis protein) transmembrane, at 3,514,483 to 3,515,142
SMc03848
SMc03849: SMc03849 - heme exporter C (cytochrome C-type biogenesis protein) transmembrane, at 3,515,202 to 3,515,972
SMc03849
SMc03850: SMc03850 - heme exporter D (cytochrome C-type biogenesis protein) transmembrane, at 3,515,969 to 3,516,145
SMc03850
SMc03851: SMc03851 - thiol:disulfide interchange protein (cytochrome C biogenesis protein), at 3,516,142 to 3,516,750
SMc03851
SMc03852: SMc03852 - hypothetical protein, at 3,516,909 to 3,517,502
SMc03852
Position (kb)
3515
3516 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3515.063 kb on + strand, within SMc03848 at 3515.158 kb on + strand at 3515.631 kb on + strand, within SMc03849 at 3515.785 kb on + strand, within SMc03849 at 3515.963 kb on + strand at 3515.963 kb on + strand at 3515.987 kb on + strand, within SMc03850 at 3515.988 kb on - strand, within SMc03850 at 3516.511 kb on + strand, within SMc03851 at 3516.748 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0001 mg/ml remove 3,515,063 + SMc03848 0.88 -1.8 3,515,158 + -0.4 3,515,631 + SMc03849 0.56 +1.2 3,515,785 + SMc03849 0.76 -3.3 3,515,963 + -0.4 3,515,963 + -0.7 3,515,987 + SMc03850 0.10 -0.8 3,515,988 - SMc03850 0.11 -2.1 3,516,511 + SMc03851 0.61 -1.6 3,516,748 + -0.6
Or see this region's nucleotide sequence