Experiment: Fusidic 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc03772 and SMc03773 are separated by 130 nucleotides SMc03773 and SMc03774 overlap by 4 nucleotides SMc03774 and SMc03775 are separated by 57 nucleotides
SMc03772: SMc03772 - 50S ribosomal protein L27, at 3,439,393 to 3,439,662
SMc03772
SMc03773: SMc03773 - hypothetical protein, at 3,439,793 to 3,440,425
SMc03773
SMc03774: SMc03774 - hypothetical protein, at 3,440,422 to 3,441,009
SMc03774
SMc03775: SMc03775 - GTPase ObgE, at 3,441,067 to 3,442,197
SMc03775
Position (kb)
3440
3441
3442 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3439.996 kb on + strand, within SMc03773 at 3439.996 kb on + strand, within SMc03773 at 3439.996 kb on + strand, within SMc03773 at 3439.997 kb on - strand, within SMc03773 at 3439.997 kb on - strand, within SMc03773 at 3440.061 kb on - strand, within SMc03773 at 3440.148 kb on - strand, within SMc03773 at 3440.181 kb on - strand, within SMc03773 at 3440.369 kb on + strand at 3440.400 kb on + strand at 3440.649 kb on + strand, within SMc03774 at 3440.767 kb on + strand, within SMc03774 at 3440.768 kb on - strand, within SMc03774 at 3440.933 kb on - strand, within SMc03774 at 3441.016 kb on - strand at 3441.090 kb on + strand at 3441.171 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0001 mg/ml remove 3,439,996 + SMc03773 0.32 -2.6 3,439,996 + SMc03773 0.32 -2.2 3,439,996 + SMc03773 0.32 -4.6 3,439,997 - SMc03773 0.32 -2.3 3,439,997 - SMc03773 0.32 -2.4 3,440,061 - SMc03773 0.42 -3.5 3,440,148 - SMc03773 0.56 -2.9 3,440,181 - SMc03773 0.61 -3.3 3,440,369 + -3.3 3,440,400 + -1.9 3,440,649 + SMc03774 0.39 -1.9 3,440,767 + SMc03774 0.59 -3.1 3,440,768 - SMc03774 0.59 -2.6 3,440,933 - SMc03774 0.87 -3.5 3,441,016 - -0.2 3,441,090 + -0.9 3,441,171 + -0.6
Or see this region's nucleotide sequence