Experiment: Fusidic 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc02847 and SMc02848 are separated by 166 nucleotides SMc02848 and SMc02849 are separated by 84 nucleotides SMc02849 and SMc02850 are separated by 139 nucleotides
SMc02847: SMc02847 - hypothetical protein, at 196,034 to 196,402
SMc02847
SMc02848: SMc02848 - hypothetical protein, at 196,569 to 197,240
SMc02848
SMc02849: SMc02849 - 2-hydroxyacid dehydrogenase, at 197,325 to 198,329
SMc02849
SMc02850: SMc02850 - DNA polymerase I, at 198,469 to 201,483
SMc02850
Position (kb) 197
198
199 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 196.392 kb on - strand at 196.513 kb on - strand at 196.513 kb on - strand at 196.570 kb on + strand at 196.570 kb on + strand at 197.276 kb on - strand at 197.276 kb on - strand at 197.276 kb on - strand at 197.353 kb on - strand at 197.611 kb on + strand, within SMc02849 at 197.791 kb on + strand, within SMc02849 at 197.791 kb on + strand, within SMc02849 at 197.792 kb on - strand, within SMc02849 at 197.792 kb on - strand, within SMc02849 at 197.792 kb on - strand, within SMc02849 at 197.994 kb on + strand, within SMc02849 at 197.994 kb on + strand, within SMc02849 at 197.994 kb on + strand, within SMc02849 at 197.994 kb on + strand, within SMc02849 at 198.042 kb on + strand, within SMc02849 at 198.042 kb on + strand, within SMc02849 at 198.043 kb on - strand, within SMc02849 at 198.043 kb on - strand, within SMc02849 at 198.043 kb on - strand, within SMc02849 at 198.043 kb on - strand, within SMc02849 at 198.213 kb on - strand, within SMc02849 at 198.346 kb on + strand at 198.396 kb on + strand at 198.429 kb on - strand at 198.471 kb on - strand at 198.471 kb on - strand at 198.866 kb on + strand, within SMc02850 at 198.867 kb on - strand, within SMc02850 at 198.896 kb on + strand, within SMc02850
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0001 mg/ml remove 196,392 - +1.2 196,513 - +0.3 196,513 - +0.5 196,570 + +1.0 196,570 + -0.1 197,276 - -2.1 197,276 - -1.9 197,276 - -0.3 197,353 - -0.6 197,611 + SMc02849 0.28 -0.8 197,791 + SMc02849 0.46 -1.0 197,791 + SMc02849 0.46 -0.5 197,792 - SMc02849 0.46 -1.3 197,792 - SMc02849 0.46 -0.5 197,792 - SMc02849 0.46 -1.0 197,994 + SMc02849 0.67 -1.5 197,994 + SMc02849 0.67 -0.9 197,994 + SMc02849 0.67 -1.0 197,994 + SMc02849 0.67 -1.6 198,042 + SMc02849 0.71 -2.6 198,042 + SMc02849 0.71 +0.1 198,043 - SMc02849 0.71 -0.5 198,043 - SMc02849 0.71 -0.1 198,043 - SMc02849 0.71 -0.8 198,043 - SMc02849 0.71 -1.2 198,213 - SMc02849 0.88 +0.1 198,346 + -0.1 198,396 + -0.6 198,429 - +0.3 198,471 - -0.3 198,471 - +0.5 198,866 + SMc02850 0.13 -5.3 198,867 - SMc02850 0.13 -2.7 198,896 + SMc02850 0.14 -3.9
Or see this region's nucleotide sequence