Experiment: Fusidic 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc02712 and SMc02713 are separated by 193 nucleotides SMc02713 and SMc02714 are separated by 3 nucleotides SMc02714 and SMc02715 are separated by 122 nucleotides SMc02715 and SMc02716 overlap by 4 nucleotides SMc02716 and SMc02717 are separated by 137 nucleotides
SMc02712: SMc02712 - hypothetical protein, at 2,561,593 to 2,562,495
SMc02712
SMc02713: SMc02713 - RNA polymerase sigma-E factor (sigma-24) protein, at 2,562,689 to 2,563,204
SMc02713
SMc02714: SMc02714 - hypothetical protein, at 2,563,208 to 2,564,035
SMc02714
SMc02715: SMc02715 - hypothetical protein, at 2,564,158 to 2,564,586
SMc02715
SMc02716: SMc02716 - hypothetical protein, at 2,564,583 to 2,564,834
SMc02716
SMc02717: SMc02717 - 2-isopropylmalate synthase, at 2,564,972 to 2,566,681
SMc02717
Position (kb)
2563
2564
2565 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2562.879 kb on - strand, within SMc02713 at 2563.002 kb on - strand, within SMc02713 at 2563.228 kb on - strand at 2563.253 kb on + strand at 2563.416 kb on + strand, within SMc02714 at 2564.086 kb on + strand at 2564.086 kb on + strand at 2564.087 kb on - strand at 2564.087 kb on - strand at 2564.106 kb on + strand at 2564.107 kb on - strand at 2564.159 kb on + strand at 2564.195 kb on + strand at 2564.196 kb on - strand at 2564.196 kb on - strand at 2564.196 kb on - strand at 2564.612 kb on + strand, within SMc02716 at 2564.613 kb on - strand, within SMc02716 at 2564.613 kb on - strand, within SMc02716 at 2564.650 kb on - strand, within SMc02716 at 2564.772 kb on + strand, within SMc02716 at 2564.825 kb on - strand at 2564.860 kb on + strand at 2564.860 kb on + strand at 2564.861 kb on - strand at 2564.861 kb on - strand at 2564.861 kb on - strand at 2564.863 kb on - strand at 2564.888 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0001 mg/ml remove 2,562,879 - SMc02713 0.37 -2.3 2,563,002 - SMc02713 0.61 -2.2 2,563,228 - -0.3 2,563,253 + -1.3 2,563,416 + SMc02714 0.25 -3.6 2,564,086 + +0.2 2,564,086 + -0.6 2,564,087 - -0.9 2,564,087 - -0.3 2,564,106 + +1.0 2,564,107 - -0.4 2,564,159 + -0.2 2,564,195 + -0.4 2,564,196 - -0.1 2,564,196 - +0.5 2,564,196 - -0.3 2,564,612 + SMc02716 0.12 +1.3 2,564,613 - SMc02716 0.12 -0.6 2,564,613 - SMc02716 0.12 +0.3 2,564,650 - SMc02716 0.27 -0.2 2,564,772 + SMc02716 0.75 +1.1 2,564,825 - -0.1 2,564,860 + -1.1 2,564,860 + -0.1 2,564,861 - +0.9 2,564,861 - +2.3 2,564,861 - +0.2 2,564,863 - +0.1 2,564,888 - +1.7
Or see this region's nucleotide sequence