Experiment: Fusidic 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc02654 and SMc02653 are separated by 159 nucleotides SMc02653 and SMc02652 overlap by 1 nucleotides SMc02652 and SMc02651 overlap by 4 nucleotides SMc02651 and SMc02650 are separated by 12 nucleotides
SMc02654: SMc02654 - 4'-phosphopantetheinyl transferase, at 1,168,355 to 1,168,774
SMc02654
SMc02653: SMc02653 - signal peptidase I transmembrane protein, at 1,168,934 to 1,169,677
SMc02653
SMc02652: SMc02652 - ribonuclease III, at 1,169,677 to 1,170,393
SMc02652
SMc02651: SMc02651 - GTP-binding protein Era, at 1,170,390 to 1,171,331
SMc02651
SMc02650: SMc02650 - hypothetical protein, at 1,171,344 to 1,172,069
SMc02650
Position (kb)
1169
1170
1171 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1168.830 kb on + strand at 1168.830 kb on + strand at 1168.832 kb on + strand at 1168.832 kb on + strand at 1168.832 kb on + strand at 1168.832 kb on + strand at 1168.832 kb on + strand at 1168.832 kb on + strand at 1168.832 kb on + strand at 1168.832 kb on + strand at 1170.398 kb on + strand at 1171.329 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0001 mg/ml remove 1,168,830 + +0.3 1,168,830 + +0.9 1,168,832 + -1.9 1,168,832 + +0.4 1,168,832 + +0.4 1,168,832 + +0.4 1,168,832 + -2.8 1,168,832 + +0.1 1,168,832 + -0.1 1,168,832 + +0.6 1,170,398 + -0.0 1,171,329 + +0.2
Or see this region's nucleotide sequence