Experiment: Fusidic 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa2139 and SMa2141 are separated by 93 nucleotides SMa2141 and SMa2143 overlap by 4 nucleotides SMa2143 and SMa2145 are separated by 55 nucleotides
SMa2139: SMa2139 - SgaA serine-glyoxylate aminotransferase (SGAT), at 1,208,866 to 1,210,071
SMa2139
SMa2141: SMa2141 - formyltetrahydrofolate deformylase, at 1,210,165 to 1,211,025
SMa2141
SMa2143: SMa2143 - 5,10-methylenetetrahydrofolate reductase, at 1,211,022 to 1,211,975
SMa2143
SMa2145: SMa2145 - glycine cleavage system protein T, at 1,212,031 to 1,213,287
SMa2145
Position (kb)
1211
1212 Strain fitness (log2 ratio)
-2
-1
0
1 at 1210.059 kb on + strand at 1210.355 kb on + strand, within SMa2141 at 1210.355 kb on + strand, within SMa2141 at 1210.506 kb on - strand, within SMa2141 at 1210.608 kb on - strand, within SMa2141 at 1210.905 kb on - strand, within SMa2141 at 1210.934 kb on - strand, within SMa2141 at 1211.132 kb on + strand, within SMa2143 at 1211.133 kb on - strand, within SMa2143 at 1211.221 kb on - strand, within SMa2143 at 1211.221 kb on - strand, within SMa2143 at 1211.278 kb on - strand, within SMa2143 at 1211.435 kb on + strand, within SMa2143 at 1211.517 kb on - strand, within SMa2143 at 1211.647 kb on + strand, within SMa2143 at 1211.647 kb on + strand, within SMa2143 at 1211.648 kb on - strand, within SMa2143 at 1211.712 kb on - strand, within SMa2143 at 1211.848 kb on - strand, within SMa2143 at 1211.886 kb on - strand at 1212.029 kb on - strand at 1212.250 kb on + strand, within SMa2145 at 1212.258 kb on - strand, within SMa2145 at 1212.362 kb on + strand, within SMa2145 at 1212.511 kb on - strand, within SMa2145 at 1212.511 kb on - strand, within SMa2145 at 1212.511 kb on - strand, within SMa2145 at 1212.513 kb on - strand, within SMa2145 at 1212.513 kb on - strand, within SMa2145 at 1212.544 kb on + strand, within SMa2145 at 1212.545 kb on - strand, within SMa2145 at 1212.589 kb on - strand, within SMa2145 at 1212.803 kb on - strand, within SMa2145 at 1212.818 kb on + strand, within SMa2145 at 1212.819 kb on - strand, within SMa2145 at 1212.909 kb on + strand, within SMa2145 at 1212.910 kb on - strand, within SMa2145 at 1212.910 kb on - strand, within SMa2145 at 1212.951 kb on - strand, within SMa2145
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0001 mg/ml remove 1,210,059 + +1.7 1,210,355 + SMa2141 0.22 +0.3 1,210,355 + SMa2141 0.22 +0.1 1,210,506 - SMa2141 0.40 -0.3 1,210,608 - SMa2141 0.51 +0.6 1,210,905 - SMa2141 0.86 +0.9 1,210,934 - SMa2141 0.89 -0.5 1,211,132 + SMa2143 0.12 -0.1 1,211,133 - SMa2143 0.12 -0.2 1,211,221 - SMa2143 0.21 -0.1 1,211,221 - SMa2143 0.21 +0.1 1,211,278 - SMa2143 0.27 -0.4 1,211,435 + SMa2143 0.43 +1.7 1,211,517 - SMa2143 0.52 -0.3 1,211,647 + SMa2143 0.66 +1.2 1,211,647 + SMa2143 0.66 -0.2 1,211,648 - SMa2143 0.66 +0.2 1,211,712 - SMa2143 0.72 +0.4 1,211,848 - SMa2143 0.87 +1.5 1,211,886 - -0.0 1,212,029 - -0.4 1,212,250 + SMa2145 0.17 +0.1 1,212,258 - SMa2145 0.18 -0.9 1,212,362 + SMa2145 0.26 -0.1 1,212,511 - SMa2145 0.38 +0.1 1,212,511 - SMa2145 0.38 +0.4 1,212,511 - SMa2145 0.38 -1.6 1,212,513 - SMa2145 0.38 -0.0 1,212,513 - SMa2145 0.38 +0.1 1,212,544 + SMa2145 0.41 +0.5 1,212,545 - SMa2145 0.41 -0.0 1,212,589 - SMa2145 0.44 -0.1 1,212,803 - SMa2145 0.61 -0.1 1,212,818 + SMa2145 0.63 +0.2 1,212,819 - SMa2145 0.63 +0.3 1,212,909 + SMa2145 0.70 -0.3 1,212,910 - SMa2145 0.70 -0.4 1,212,910 - SMa2145 0.70 +0.1 1,212,951 - SMa2145 0.73 -2.2
Or see this region's nucleotide sequence