Experiment: Fusidic 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa2113 and SMa2115 are separated by 48 nucleotides SMa2115 and SMa2117 are separated by 11 nucleotides SMa2117 and SMa2119 are separated by 42 nucleotides SMa2119 and SMa2121 are separated by 15 nucleotides
SMa2113: SMa2113 - glutathione-dependent dehydrogenase, at 1,193,878 to 1,195,005
SMa2113
SMa2115: SMa2115 - Gst13 glutathione S-transferase, at 1,195,054 to 1,195,701
SMa2115
SMa2117: SMa2117 - oxidoreductase, at 1,195,713 to 1,197,479
SMa2117
SMa2119: SMa2119 - hypothetical protein, at 1,197,522 to 1,198,448
SMa2119
SMa2121: SMa2121 - hypothetical protein, at 1,198,464 to 1,199,327
SMa2121
Position (kb)
1195
1196
1197
1198 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1194.744 kb on - strand, within SMa2113 at 1195.007 kb on + strand at 1195.008 kb on - strand at 1195.008 kb on - strand at 1195.008 kb on - strand at 1195.194 kb on - strand, within SMa2115 at 1195.194 kb on - strand, within SMa2115 at 1195.210 kb on - strand, within SMa2115 at 1195.383 kb on + strand, within SMa2115 at 1195.383 kb on + strand, within SMa2115 at 1195.383 kb on + strand, within SMa2115 at 1195.383 kb on + strand, within SMa2115 at 1195.384 kb on - strand, within SMa2115 at 1195.384 kb on - strand, within SMa2115 at 1195.448 kb on + strand, within SMa2115 at 1195.473 kb on + strand, within SMa2115 at 1195.474 kb on - strand, within SMa2115 at 1195.599 kb on - strand, within SMa2115 at 1195.622 kb on + strand, within SMa2115 at 1195.622 kb on + strand, within SMa2115 at 1195.623 kb on - strand, within SMa2115 at 1195.645 kb on - strand at 1195.645 kb on - strand at 1195.645 kb on - strand at 1195.688 kb on + strand at 1195.688 kb on + strand at 1195.715 kb on - strand at 1195.940 kb on + strand, within SMa2117 at 1195.983 kb on - strand, within SMa2117 at 1195.983 kb on - strand, within SMa2117 at 1195.983 kb on - strand, within SMa2117 at 1196.011 kb on + strand, within SMa2117 at 1196.099 kb on - strand, within SMa2117 at 1196.099 kb on - strand, within SMa2117 at 1196.099 kb on - strand, within SMa2117 at 1196.099 kb on - strand, within SMa2117 at 1196.099 kb on - strand, within SMa2117 at 1196.336 kb on + strand, within SMa2117 at 1196.336 kb on + strand, within SMa2117 at 1196.387 kb on + strand, within SMa2117 at 1196.538 kb on + strand, within SMa2117 at 1196.538 kb on + strand, within SMa2117 at 1196.538 kb on + strand, within SMa2117 at 1196.539 kb on - strand, within SMa2117 at 1196.539 kb on - strand, within SMa2117 at 1196.610 kb on - strand, within SMa2117 at 1196.862 kb on + strand, within SMa2117 at 1196.862 kb on + strand, within SMa2117 at 1197.017 kb on - strand, within SMa2117 at 1197.063 kb on + strand at 1197.155 kb on - strand, within SMa2117 at 1197.226 kb on + strand, within SMa2117 at 1197.254 kb on - strand, within SMa2117 at 1197.309 kb on + strand at 1197.355 kb on + strand at 1197.581 kb on - strand at 1197.717 kb on + strand, within SMa2119 at 1197.850 kb on + strand, within SMa2119 at 1197.868 kb on + strand, within SMa2119 at 1197.869 kb on - strand, within SMa2119 at 1197.874 kb on + strand, within SMa2119 at 1197.874 kb on + strand, within SMa2119 at 1197.874 kb on + strand, within SMa2119 at 1197.874 kb on + strand, within SMa2119 at 1198.047 kb on - strand, within SMa2119 at 1198.192 kb on + strand, within SMa2119 at 1198.193 kb on - strand, within SMa2119 at 1198.193 kb on - strand, within SMa2119 at 1198.327 kb on - strand, within SMa2119 at 1198.386 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0001 mg/ml remove 1,194,744 - SMa2113 0.77 +0.3 1,195,007 + +0.2 1,195,008 - +0.7 1,195,008 - -1.3 1,195,008 - -0.2 1,195,194 - SMa2115 0.22 +0.7 1,195,194 - SMa2115 0.22 -0.7 1,195,210 - SMa2115 0.24 +0.3 1,195,383 + SMa2115 0.51 +0.3 1,195,383 + SMa2115 0.51 +0.7 1,195,383 + SMa2115 0.51 -0.0 1,195,383 + SMa2115 0.51 -0.5 1,195,384 - SMa2115 0.51 +0.8 1,195,384 - SMa2115 0.51 -0.0 1,195,448 + SMa2115 0.61 -0.2 1,195,473 + SMa2115 0.65 +0.7 1,195,474 - SMa2115 0.65 +1.4 1,195,599 - SMa2115 0.84 -0.7 1,195,622 + SMa2115 0.88 -0.2 1,195,622 + SMa2115 0.88 +0.4 1,195,623 - SMa2115 0.88 +0.9 1,195,645 - -1.4 1,195,645 - +0.6 1,195,645 - +0.7 1,195,688 + +0.2 1,195,688 + +1.4 1,195,715 - -1.4 1,195,940 + SMa2117 0.13 +0.2 1,195,983 - SMa2117 0.15 -0.6 1,195,983 - SMa2117 0.15 -0.6 1,195,983 - SMa2117 0.15 +0.4 1,196,011 + SMa2117 0.17 -1.0 1,196,099 - SMa2117 0.22 -1.2 1,196,099 - SMa2117 0.22 -0.2 1,196,099 - SMa2117 0.22 +0.3 1,196,099 - SMa2117 0.22 -0.3 1,196,099 - SMa2117 0.22 -0.1 1,196,336 + SMa2117 0.35 -0.0 1,196,336 + SMa2117 0.35 -0.4 1,196,387 + SMa2117 0.38 +1.0 1,196,538 + SMa2117 0.47 -1.1 1,196,538 + SMa2117 0.47 -1.6 1,196,538 + SMa2117 0.47 +0.0 1,196,539 - SMa2117 0.47 -0.4 1,196,539 - SMa2117 0.47 -2.8 1,196,610 - SMa2117 0.51 -0.3 1,196,862 + SMa2117 0.65 -1.4 1,196,862 + SMa2117 0.65 +1.0 1,197,017 - SMa2117 0.74 -0.6 1,197,063 + +1.0 1,197,155 - SMa2117 0.82 -0.3 1,197,226 + SMa2117 0.86 +0.9 1,197,254 - SMa2117 0.87 -1.4 1,197,309 + +0.0 1,197,355 + -0.1 1,197,581 - +0.4 1,197,717 + SMa2119 0.21 +0.6 1,197,850 + SMa2119 0.35 -1.7 1,197,868 + SMa2119 0.37 -0.2 1,197,869 - SMa2119 0.37 +0.4 1,197,874 + SMa2119 0.38 +0.6 1,197,874 + SMa2119 0.38 -1.8 1,197,874 + SMa2119 0.38 +0.7 1,197,874 + SMa2119 0.38 +0.7 1,198,047 - SMa2119 0.57 +0.8 1,198,192 + SMa2119 0.72 -0.2 1,198,193 - SMa2119 0.72 +0.1 1,198,193 - SMa2119 0.72 -0.1 1,198,327 - SMa2119 0.87 +0.3 1,198,386 + -0.3
Or see this region's nucleotide sequence