Experiment: Fusidic 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa1933 and SMa1935 are separated by 154 nucleotides SMa1935 and SMa1937 overlap by 4 nucleotides SMa1937 and SMa1939 are separated by 175 nucleotides
SMa1933: SMa1933 - LysR family transcriptional regulator, at 1,098,786 to 1,099,427
SMa1933
SMa1935: SMa1935 - TrpR binding protein WrbA, at 1,099,582 to 1,100,220
SMa1935
SMa1937: SMa1937 - hypothetical protein, at 1,100,217 to 1,101,455
SMa1937
SMa1939: SMa1939 - hypothetical protein, at 1,101,631 to 1,103,166
SMa1939
Position (kb)
1100
1101
1102 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1099.225 kb on - strand, within SMa1933 at 1099.432 kb on + strand at 1099.432 kb on + strand at 1099.432 kb on + strand at 1099.440 kb on - strand at 1099.468 kb on + strand at 1099.494 kb on + strand at 1099.494 kb on + strand at 1099.495 kb on - strand at 1099.607 kb on - strand at 1099.616 kb on - strand at 1099.787 kb on - strand, within SMa1935 at 1100.006 kb on + strand, within SMa1935 at 1100.006 kb on + strand, within SMa1935 at 1100.007 kb on - strand, within SMa1935 at 1100.007 kb on - strand, within SMa1935 at 1100.035 kb on - strand, within SMa1935 at 1100.063 kb on - strand, within SMa1935 at 1100.309 kb on + strand at 1100.668 kb on + strand, within SMa1937 at 1100.668 kb on + strand, within SMa1937 at 1100.755 kb on - strand, within SMa1937 at 1100.875 kb on + strand, within SMa1937 at 1100.876 kb on - strand, within SMa1937 at 1101.029 kb on - strand, within SMa1937 at 1101.114 kb on - strand, within SMa1937 at 1101.135 kb on + strand, within SMa1937 at 1101.431 kb on + strand at 1101.432 kb on - strand at 1101.524 kb on - strand at 1101.627 kb on - strand at 1101.627 kb on - strand at 1101.632 kb on + strand at 1101.632 kb on + strand at 1101.632 kb on + strand at 1101.632 kb on + strand at 1101.652 kb on - strand at 1101.652 kb on - strand at 1101.667 kb on - strand at 1101.747 kb on - strand at 1101.747 kb on - strand at 1101.747 kb on - strand at 1101.865 kb on - strand, within SMa1939 at 1101.919 kb on + strand, within SMa1939 at 1101.926 kb on + strand, within SMa1939 at 1101.927 kb on - strand, within SMa1939 at 1101.934 kb on + strand, within SMa1939 at 1102.187 kb on + strand, within SMa1939 at 1102.188 kb on - strand, within SMa1939 at 1102.203 kb on + strand, within SMa1939 at 1102.347 kb on - strand, within SMa1939
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0001 mg/ml remove 1,099,225 - SMa1933 0.68 -0.5 1,099,432 + -0.8 1,099,432 + -0.2 1,099,432 + -1.4 1,099,440 - +0.1 1,099,468 + -1.2 1,099,494 + +0.6 1,099,494 + -0.2 1,099,495 - -0.3 1,099,607 - +0.1 1,099,616 - -0.0 1,099,787 - SMa1935 0.32 -1.6 1,100,006 + SMa1935 0.66 -0.3 1,100,006 + SMa1935 0.66 +0.0 1,100,007 - SMa1935 0.67 -0.1 1,100,007 - SMa1935 0.67 -0.6 1,100,035 - SMa1935 0.71 -0.1 1,100,063 - SMa1935 0.75 -0.4 1,100,309 + -0.6 1,100,668 + SMa1937 0.36 -0.4 1,100,668 + SMa1937 0.36 +0.0 1,100,755 - SMa1937 0.43 +0.9 1,100,875 + SMa1937 0.53 +0.4 1,100,876 - SMa1937 0.53 +0.2 1,101,029 - SMa1937 0.66 -0.2 1,101,114 - SMa1937 0.72 +1.0 1,101,135 + SMa1937 0.74 +0.8 1,101,431 + -0.4 1,101,432 - -0.2 1,101,524 - -0.1 1,101,627 - -0.6 1,101,627 - -0.6 1,101,632 + +0.2 1,101,632 + -0.8 1,101,632 + -0.4 1,101,632 + -0.2 1,101,652 - -0.6 1,101,652 - -1.0 1,101,667 - +0.3 1,101,747 - -3.4 1,101,747 - +0.4 1,101,747 - +0.3 1,101,865 - SMa1939 0.15 +0.9 1,101,919 + SMa1939 0.19 +0.0 1,101,926 + SMa1939 0.19 +0.2 1,101,927 - SMa1939 0.19 -0.9 1,101,934 + SMa1939 0.20 -0.6 1,102,187 + SMa1939 0.36 +1.1 1,102,188 - SMa1939 0.36 +0.2 1,102,203 + SMa1939 0.37 -0.7 1,102,347 - SMa1939 0.47 -0.7
Or see this region's nucleotide sequence