Experiment: Fusidic 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0775 and SMa0776 are separated by 405 nucleotides SMa0776 and SMa0779 are separated by 12 nucleotides SMa0779 and SMa5028 overlap by 183 nucleotides
SMa0775: SMa0775 - hypothetical protein, at 422,708 to 423,034
SMa0775
SMa0776: SMa0776 - TRm23b IS ATP-binding protein, at 423,440 to 424,177
SMa0776
SMa0779: SMa0779 - hypothetical protein, at 424,190 to 425,086
SMa0779
SMa5028: SMa5028 - hypothetical protein, at 424,904 to 425,281
SMa5028
Position (kb) 423
424
425 Strain fitness (log2 ratio) -1
0
1 at 422.559 kb on - strand at 422.578 kb on - strand at 422.589 kb on + strand at 422.616 kb on + strand at 422.616 kb on + strand at 422.619 kb on - strand at 422.622 kb on + strand at 422.679 kb on + strand at 422.847 kb on - strand, within SMa0775 at 422.927 kb on - strand, within SMa0775 at 422.990 kb on + strand, within SMa0775 at 422.991 kb on - strand, within SMa0775 at 423.140 kb on + strand at 423.140 kb on + strand at 423.140 kb on + strand at 423.140 kb on + strand at 423.307 kb on + strand at 423.549 kb on - strand, within SMa0776 at 424.540 kb on + strand, within SMa0779 at 424.540 kb on + strand, within SMa0779 at 424.752 kb on + strand, within SMa0779 at 424.898 kb on + strand, within SMa0779 at 424.900 kb on + strand, within SMa0779 at 425.000 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0001 mg/ml remove 422,559 - +0.3 422,578 - +0.3 422,589 + -0.1 422,616 + +0.3 422,616 + -0.7 422,619 - +0.3 422,622 + -0.3 422,679 + -0.2 422,847 - SMa0775 0.43 -0.2 422,927 - SMa0775 0.67 +0.2 422,990 + SMa0775 0.86 +0.3 422,991 - SMa0775 0.87 -0.2 423,140 + -0.3 423,140 + -1.1 423,140 + -0.7 423,140 + +0.2 423,307 + +0.5 423,549 - SMa0776 0.15 +0.6 424,540 + SMa0779 0.39 -0.7 424,540 + SMa0779 0.39 +0.5 424,752 + SMa0779 0.63 +0.1 424,898 + SMa0779 0.79 -0.3 424,900 + SMa0779 0.79 -0.5 425,000 - -0.0
Or see this region's nucleotide sequence