Experiment: Fusidic 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0322 and SMa0323 are separated by 50 nucleotides SMa0323 and SMa0325 are separated by 100 nucleotides SMa0325 and SMa0326 are separated by 20 nucleotides SMa0326 and SMa0329 are separated by 72 nucleotides SMa0329 and SMa5017 are separated by 55 nucleotides
SMa0322: SMa0322 - hypothetical protein, at 179,926 to 180,474
SMa0322
SMa0323: SMa0323 - hypothetical protein, at 180,525 to 180,809
SMa0323
SMa0325: SMa0325 - hypothetical protein, at 180,910 to 181,212
SMa0325
SMa0326: SMa0326 - short chain alcohol dehydrogenase-related dehydrogenase, at 181,233 to 182,072
SMa0326
SMa0329: SMa0329 - short chain alcohol dehydrogenase-related dehydrogenase, at 182,145 to 182,906
SMa0329
SMa5017: SMa5017 - hypothetical protein, at 182,962 to 183,186
SMa5017
Position (kb) 181
182
183 Strain fitness (log2 ratio) -2
-1
0
1 at 180.276 kb on + strand, within SMa0322 at 180.277 kb on - strand, within SMa0322 at 180.279 kb on + strand, within SMa0322 at 180.319 kb on + strand, within SMa0322 at 180.369 kb on + strand, within SMa0322 at 180.370 kb on - strand, within SMa0322 at 180.370 kb on - strand, within SMa0322 at 180.405 kb on + strand, within SMa0322 at 180.467 kb on + strand at 180.468 kb on - strand at 180.525 kb on - strand at 180.525 kb on - strand at 180.534 kb on + strand at 180.550 kb on + strand at 180.556 kb on + strand, within SMa0323 at 180.556 kb on + strand, within SMa0323 at 180.557 kb on - strand, within SMa0323 at 180.557 kb on - strand, within SMa0323 at 180.557 kb on - strand, within SMa0323 at 180.741 kb on + strand, within SMa0323 at 180.790 kb on + strand at 180.791 kb on - strand at 180.889 kb on + strand at 180.889 kb on + strand at 180.889 kb on + strand at 180.890 kb on - strand at 180.935 kb on - strand at 180.935 kb on - strand at 181.110 kb on + strand, within SMa0325 at 181.124 kb on - strand, within SMa0325 at 181.214 kb on - strand at 181.225 kb on + strand at 181.226 kb on - strand at 181.277 kb on + strand at 181.277 kb on + strand at 181.278 kb on - strand at 181.601 kb on + strand, within SMa0326 at 181.677 kb on + strand, within SMa0326 at 181.680 kb on + strand, within SMa0326 at 181.680 kb on + strand, within SMa0326 at 181.680 kb on + strand, within SMa0326 at 181.681 kb on - strand, within SMa0326 at 181.867 kb on - strand, within SMa0326 at 182.499 kb on + strand, within SMa0329 at 182.500 kb on - strand, within SMa0329 at 182.500 kb on - strand, within SMa0329 at 182.682 kb on - strand, within SMa0329 at 182.700 kb on - strand, within SMa0329
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0001 mg/ml remove 180,276 + SMa0322 0.64 -0.5 180,277 - SMa0322 0.64 -0.8 180,279 + SMa0322 0.64 -1.1 180,319 + SMa0322 0.72 -0.2 180,369 + SMa0322 0.81 -0.0 180,370 - SMa0322 0.81 +0.6 180,370 - SMa0322 0.81 -0.5 180,405 + SMa0322 0.87 -0.0 180,467 + -0.4 180,468 - +0.2 180,525 - +0.6 180,525 - -0.2 180,534 + +0.8 180,550 + -0.4 180,556 + SMa0323 0.11 -0.2 180,556 + SMa0323 0.11 -2.0 180,557 - SMa0323 0.11 -0.5 180,557 - SMa0323 0.11 -0.1 180,557 - SMa0323 0.11 -0.0 180,741 + SMa0323 0.76 -0.2 180,790 + -1.2 180,791 - -0.1 180,889 + +0.4 180,889 + -0.5 180,889 + +0.2 180,890 - -0.7 180,935 - -0.2 180,935 - -0.1 181,110 + SMa0325 0.66 -0.5 181,124 - SMa0325 0.71 -0.1 181,214 - -0.5 181,225 + -0.1 181,226 - +0.5 181,277 + +0.6 181,277 + -0.2 181,278 - +0.1 181,601 + SMa0326 0.44 -0.7 181,677 + SMa0326 0.53 +1.1 181,680 + SMa0326 0.53 +1.1 181,680 + SMa0326 0.53 +1.4 181,680 + SMa0326 0.53 +0.6 181,681 - SMa0326 0.53 -0.2 181,867 - SMa0326 0.75 +1.4 182,499 + SMa0329 0.46 -0.1 182,500 - SMa0329 0.47 +0.1 182,500 - SMa0329 0.47 -0.1 182,682 - SMa0329 0.70 +0.2 182,700 - SMa0329 0.73 -0.1
Or see this region's nucleotide sequence