Experiment: Fusidic 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0142 and SMa0143 are separated by 142 nucleotides SMa0143 and SMa0144 overlap by 4 nucleotides SMa0144 and SMa0146 are separated by 5 nucleotides SMa0146 and SMa0148 are separated by 140 nucleotides
SMa0142: SMa0142 - protease, at 77,422 to 78,822
SMa0142
SMa0143: SMa0143 - RNA polymerase ECF sigma factor, at 78,965 to 79,507
SMa0143
SMa0144: SMa0144 - hypothetical protein, at 79,504 to 80,289
SMa0144
SMa0146: SMa0146 - hypothetical protein, at 80,295 to 80,819
SMa0146
SMa0148: SMa0148 - hypothetical protein, at 80,960 to 81,673
SMa0148
Position (kb) 79
80
81 Strain fitness (log2 ratio) -1
0
1 at 78.515 kb on - strand, within SMa0142 at 78.766 kb on + strand at 78.863 kb on - strand at 78.891 kb on - strand at 78.896 kb on + strand at 78.896 kb on + strand at 78.897 kb on - strand at 78.897 kb on - strand at 78.928 kb on + strand at 78.928 kb on + strand at 78.929 kb on - strand at 79.146 kb on + strand, within SMa0143 at 79.216 kb on + strand, within SMa0143 at 79.359 kb on + strand, within SMa0143 at 79.359 kb on + strand, within SMa0143 at 79.360 kb on - strand, within SMa0143 at 79.360 kb on - strand, within SMa0143 at 79.360 kb on - strand, within SMa0143 at 79.360 kb on - strand, within SMa0143 at 79.360 kb on - strand, within SMa0143 at 79.458 kb on + strand at 79.532 kb on + strand at 79.583 kb on + strand, within SMa0144 at 80.287 kb on + strand at 80.287 kb on + strand at 80.287 kb on + strand at 80.287 kb on + strand at 80.287 kb on + strand at 80.287 kb on + strand at 80.287 kb on + strand at 80.287 kb on + strand at 80.378 kb on + strand, within SMa0146 at 80.379 kb on - strand, within SMa0146 at 80.464 kb on + strand, within SMa0146 at 80.465 kb on - strand, within SMa0146 at 80.465 kb on - strand, within SMa0146 at 80.540 kb on + strand, within SMa0146 at 80.541 kb on - strand, within SMa0146 at 80.658 kb on + strand, within SMa0146 at 80.658 kb on + strand, within SMa0146 at 80.855 kb on - strand at 80.942 kb on + strand at 80.943 kb on - strand at 80.943 kb on - strand at 80.943 kb on - strand at 81.101 kb on + strand, within SMa0148 at 81.110 kb on + strand, within SMa0148 at 81.111 kb on - strand, within SMa0148 at 81.179 kb on - strand, within SMa0148 at 81.189 kb on + strand, within SMa0148 at 81.189 kb on + strand, within SMa0148 at 81.189 kb on + strand, within SMa0148 at 81.189 kb on + strand, within SMa0148 at 81.189 kb on + strand, within SMa0148 at 81.190 kb on - strand, within SMa0148 at 81.190 kb on - strand, within SMa0148 at 81.190 kb on - strand, within SMa0148 at 81.190 kb on - strand, within SMa0148 at 81.190 kb on - strand, within SMa0148 at 81.190 kb on - strand, within SMa0148 at 81.190 kb on - strand, within SMa0148 at 81.190 kb on - strand, within SMa0148
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.0001 mg/ml remove 78,515 - SMa0142 0.78 +0.1 78,766 + -1.5 78,863 - +0.1 78,891 - +0.8 78,896 + +0.6 78,896 + +0.4 78,897 - -0.3 78,897 - -0.2 78,928 + +0.6 78,928 + -0.3 78,929 - -0.2 79,146 + SMa0143 0.33 +0.0 79,216 + SMa0143 0.46 +0.7 79,359 + SMa0143 0.73 +0.2 79,359 + SMa0143 0.73 +0.2 79,360 - SMa0143 0.73 -0.1 79,360 - SMa0143 0.73 +0.1 79,360 - SMa0143 0.73 -0.0 79,360 - SMa0143 0.73 -0.1 79,360 - SMa0143 0.73 -0.3 79,458 + -0.3 79,532 + -0.6 79,583 + SMa0144 0.10 -1.1 80,287 + +0.1 80,287 + +0.4 80,287 + -0.2 80,287 + -0.4 80,287 + +0.2 80,287 + +1.6 80,287 + -0.7 80,287 + +0.7 80,378 + SMa0146 0.16 -0.3 80,379 - SMa0146 0.16 -0.1 80,464 + SMa0146 0.32 +0.4 80,465 - SMa0146 0.32 -0.9 80,465 - SMa0146 0.32 -1.2 80,540 + SMa0146 0.47 -0.1 80,541 - SMa0146 0.47 -0.1 80,658 + SMa0146 0.69 +0.0 80,658 + SMa0146 0.69 -0.1 80,855 - -0.6 80,942 + -0.7 80,943 - -0.4 80,943 - +0.1 80,943 - -0.4 81,101 + SMa0148 0.20 +0.0 81,110 + SMa0148 0.21 -1.2 81,111 - SMa0148 0.21 -0.4 81,179 - SMa0148 0.31 -0.0 81,189 + SMa0148 0.32 -0.3 81,189 + SMa0148 0.32 +0.6 81,189 + SMa0148 0.32 +0.1 81,189 + SMa0148 0.32 -0.3 81,189 + SMa0148 0.32 +0.7 81,190 - SMa0148 0.32 +0.2 81,190 - SMa0148 0.32 +0.5 81,190 - SMa0148 0.32 -0.5 81,190 - SMa0148 0.32 +0.1 81,190 - SMa0148 0.32 -1.6 81,190 - SMa0148 0.32 -1.0 81,190 - SMa0148 0.32 +0.6 81,190 - SMa0148 0.32 +0.4
Or see this region's nucleotide sequence