Experiment: L-Tryptophan (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00950 and RR42_RS00955 are separated by 118 nucleotides RR42_RS00955 and RR42_RS00960 are separated by 79 nucleotides RR42_RS00960 and RR42_RS00965 are separated by 60 nucleotides RR42_RS00965 and RR42_RS00970 are separated by 12 nucleotides
RR42_RS00950: RR42_RS00950 - glyoxylate reductase, at 226,613 to 227,554
_RS00950
RR42_RS00955: RR42_RS00955 - hydroxymethylglutaryl-CoA lyase, at 227,673 to 228,608
_RS00955
RR42_RS00960: RR42_RS00960 - prolyl-tRNA synthetase, at 228,688 to 229,167
_RS00960
RR42_RS00965: RR42_RS00965 - Fe-S oxidoreductase, at 229,228 to 229,497
_RS00965
RR42_RS00970: RR42_RS00970 - DSBA oxidoreductase, at 229,510 to 230,199
_RS00970
Position (kb)
227
228
229 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 226.733 kb on + strand, within RR42_RS00950 at 226.734 kb on - strand, within RR42_RS00950 at 226.919 kb on + strand, within RR42_RS00950 at 226.964 kb on + strand, within RR42_RS00950 at 226.965 kb on - strand, within RR42_RS00950 at 226.965 kb on - strand, within RR42_RS00950 at 226.965 kb on - strand, within RR42_RS00950 at 227.276 kb on + strand, within RR42_RS00950 at 227.277 kb on - strand, within RR42_RS00950 at 227.440 kb on + strand, within RR42_RS00950 at 227.441 kb on - strand, within RR42_RS00950 at 227.614 kb on + strand at 227.615 kb on - strand at 227.693 kb on - strand at 227.805 kb on - strand, within RR42_RS00955 at 227.901 kb on + strand, within RR42_RS00955 at 227.921 kb on + strand, within RR42_RS00955 at 227.922 kb on - strand, within RR42_RS00955 at 227.922 kb on - strand, within RR42_RS00955 at 228.158 kb on - strand, within RR42_RS00955 at 228.158 kb on - strand, within RR42_RS00955 at 228.307 kb on - strand, within RR42_RS00955 at 228.307 kb on - strand, within RR42_RS00955 at 228.441 kb on + strand, within RR42_RS00955 at 228.441 kb on + strand, within RR42_RS00955 at 228.442 kb on - strand, within RR42_RS00955 at 228.442 kb on - strand, within RR42_RS00955 at 228.604 kb on + strand at 228.604 kb on + strand at 228.604 kb on + strand at 228.605 kb on - strand at 228.606 kb on + strand at 228.606 kb on + strand at 228.607 kb on - strand at 228.607 kb on - strand at 228.634 kb on - strand at 228.634 kb on - strand at 228.667 kb on - strand at 228.996 kb on + strand, within RR42_RS00960 at 228.996 kb on + strand, within RR42_RS00960 at 229.181 kb on - strand at 229.181 kb on - strand at 229.181 kb on - strand at 229.345 kb on + strand, within RR42_RS00965 at 229.495 kb on + strand at 229.495 kb on + strand at 229.495 kb on + strand at 229.495 kb on + strand at 229.496 kb on - strand at 229.496 kb on - strand at 229.496 kb on - strand at 229.496 kb on - strand at 229.496 kb on - strand at 229.496 kb on - strand at 229.531 kb on + strand at 229.532 kb on - strand at 229.558 kb on + strand at 229.559 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Tryptophan (C) remove 226,733 + RR42_RS00950 0.13 -1.4 226,734 - RR42_RS00950 0.13 -0.4 226,919 + RR42_RS00950 0.32 +1.1 226,964 + RR42_RS00950 0.37 +1.1 226,965 - RR42_RS00950 0.37 -0.7 226,965 - RR42_RS00950 0.37 -0.3 226,965 - RR42_RS00950 0.37 -0.1 227,276 + RR42_RS00950 0.70 +2.1 227,277 - RR42_RS00950 0.70 +0.4 227,440 + RR42_RS00950 0.88 +0.9 227,441 - RR42_RS00950 0.88 +0.2 227,614 + -1.4 227,615 - +0.5 227,693 - +0.3 227,805 - RR42_RS00955 0.14 -0.4 227,901 + RR42_RS00955 0.24 -0.2 227,921 + RR42_RS00955 0.26 -1.3 227,922 - RR42_RS00955 0.27 -1.1 227,922 - RR42_RS00955 0.27 -0.4 228,158 - RR42_RS00955 0.52 -0.7 228,158 - RR42_RS00955 0.52 -1.5 228,307 - RR42_RS00955 0.68 -1.3 228,307 - RR42_RS00955 0.68 +0.0 228,441 + RR42_RS00955 0.82 -2.6 228,441 + RR42_RS00955 0.82 -1.6 228,442 - RR42_RS00955 0.82 -2.0 228,442 - RR42_RS00955 0.82 -0.4 228,604 + -1.3 228,604 + +0.6 228,604 + +0.3 228,605 - +0.6 228,606 + +1.1 228,606 + +0.0 228,607 - +0.6 228,607 - +1.9 228,634 - -0.4 228,634 - +0.0 228,667 - -0.8 228,996 + RR42_RS00960 0.64 +1.4 228,996 + RR42_RS00960 0.64 -0.3 229,181 - -0.4 229,181 - -1.8 229,181 - -0.6 229,345 + RR42_RS00965 0.43 -0.8 229,495 + +0.8 229,495 + -0.1 229,495 + +0.5 229,495 + +2.3 229,496 - -0.6 229,496 - -0.0 229,496 - +0.1 229,496 - -1.3 229,496 - -0.5 229,496 - +0.7 229,531 + +0.1 229,532 - -2.7 229,558 + +0.6 229,559 - +0.1
Or see this region's nucleotide sequence