Experiment: L-Tryptophan (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00870 and RR42_RS00875 are separated by 161 nucleotides RR42_RS00875 and RR42_RS00880 overlap by 4 nucleotides RR42_RS00880 and RR42_RS00885 are separated by 93 nucleotides
RR42_RS00870: RR42_RS00870 - isovaleryl-CoA dehydrogenase, at 209,849 to 211,039
_RS00870
RR42_RS00875: RR42_RS00875 - isocitrate dehydrogenase, at 211,201 to 213,045
_RS00875
RR42_RS00880: RR42_RS00880 - carbonate dehydratase, at 213,042 to 213,713
_RS00880
RR42_RS00885: RR42_RS00885 - acetyl-CoA acetyltransferase, at 213,807 to 215,003
_RS00885
Position (kb) 211
212
213
214 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 211.155 kb on + strand at 211.184 kb on - strand at 211.240 kb on + strand at 211.381 kb on + strand at 211.381 kb on + strand at 211.426 kb on + strand, within RR42_RS00875 at 211.426 kb on + strand, within RR42_RS00875 at 211.427 kb on - strand, within RR42_RS00875 at 211.427 kb on - strand, within RR42_RS00875 at 211.441 kb on - strand, within RR42_RS00875 at 211.477 kb on + strand, within RR42_RS00875 at 211.478 kb on - strand, within RR42_RS00875 at 211.478 kb on - strand, within RR42_RS00875 at 211.564 kb on + strand, within RR42_RS00875 at 211.564 kb on + strand, within RR42_RS00875 at 211.565 kb on - strand, within RR42_RS00875 at 211.565 kb on - strand, within RR42_RS00875 at 211.612 kb on + strand, within RR42_RS00875 at 211.612 kb on + strand, within RR42_RS00875 at 211.612 kb on + strand, within RR42_RS00875 at 211.613 kb on - strand, within RR42_RS00875 at 211.658 kb on - strand, within RR42_RS00875 at 211.661 kb on - strand, within RR42_RS00875 at 211.711 kb on - strand, within RR42_RS00875 at 211.752 kb on + strand, within RR42_RS00875 at 211.759 kb on + strand, within RR42_RS00875 at 211.760 kb on - strand, within RR42_RS00875 at 211.760 kb on - strand, within RR42_RS00875 at 212.038 kb on + strand, within RR42_RS00875 at 212.038 kb on + strand, within RR42_RS00875 at 212.039 kb on - strand, within RR42_RS00875 at 212.039 kb on - strand, within RR42_RS00875 at 212.039 kb on - strand, within RR42_RS00875 at 212.090 kb on - strand, within RR42_RS00875 at 212.090 kb on - strand, within RR42_RS00875 at 212.236 kb on + strand, within RR42_RS00875 at 212.236 kb on + strand, within RR42_RS00875 at 212.236 kb on + strand, within RR42_RS00875 at 212.237 kb on - strand, within RR42_RS00875 at 212.261 kb on - strand, within RR42_RS00875 at 212.261 kb on - strand, within RR42_RS00875 at 212.309 kb on - strand, within RR42_RS00875 at 212.447 kb on - strand, within RR42_RS00875 at 212.494 kb on + strand, within RR42_RS00875 at 212.494 kb on + strand, within RR42_RS00875 at 212.495 kb on - strand, within RR42_RS00875 at 212.495 kb on - strand, within RR42_RS00875 at 212.496 kb on + strand, within RR42_RS00875 at 212.496 kb on + strand, within RR42_RS00875 at 212.610 kb on + strand, within RR42_RS00875 at 212.632 kb on + strand, within RR42_RS00875 at 212.632 kb on + strand, within RR42_RS00875 at 212.632 kb on + strand, within RR42_RS00875 at 212.632 kb on + strand, within RR42_RS00875 at 212.632 kb on + strand, within RR42_RS00875 at 212.941 kb on + strand at 212.941 kb on + strand at 212.941 kb on + strand at 212.941 kb on + strand at 213.739 kb on + strand at 213.739 kb on + strand at 213.743 kb on + strand at 213.743 kb on + strand at 213.744 kb on - strand at 213.820 kb on + strand at 213.820 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Tryptophan (C) remove 211,155 + -0.2 211,184 - -1.3 211,240 + +0.1 211,381 + -0.1 211,381 + -0.3 211,426 + RR42_RS00875 0.12 +0.9 211,426 + RR42_RS00875 0.12 -3.4 211,427 - RR42_RS00875 0.12 -0.2 211,427 - RR42_RS00875 0.12 -1.2 211,441 - RR42_RS00875 0.13 -1.9 211,477 + RR42_RS00875 0.15 -0.7 211,478 - RR42_RS00875 0.15 -0.3 211,478 - RR42_RS00875 0.15 -1.0 211,564 + RR42_RS00875 0.20 -1.6 211,564 + RR42_RS00875 0.20 -1.3 211,565 - RR42_RS00875 0.20 -2.3 211,565 - RR42_RS00875 0.20 +0.1 211,612 + RR42_RS00875 0.22 -0.4 211,612 + RR42_RS00875 0.22 +0.8 211,612 + RR42_RS00875 0.22 -1.9 211,613 - RR42_RS00875 0.22 -0.1 211,658 - RR42_RS00875 0.25 -0.9 211,661 - RR42_RS00875 0.25 -0.7 211,711 - RR42_RS00875 0.28 -0.6 211,752 + RR42_RS00875 0.30 +0.2 211,759 + RR42_RS00875 0.30 -0.0 211,760 - RR42_RS00875 0.30 +1.1 211,760 - RR42_RS00875 0.30 -3.0 212,038 + RR42_RS00875 0.45 -1.3 212,038 + RR42_RS00875 0.45 +0.3 212,039 - RR42_RS00875 0.45 -1.2 212,039 - RR42_RS00875 0.45 +0.5 212,039 - RR42_RS00875 0.45 -1.3 212,090 - RR42_RS00875 0.48 +0.6 212,090 - RR42_RS00875 0.48 -0.8 212,236 + RR42_RS00875 0.56 -0.6 212,236 + RR42_RS00875 0.56 -0.2 212,236 + RR42_RS00875 0.56 -0.8 212,237 - RR42_RS00875 0.56 -1.2 212,261 - RR42_RS00875 0.57 -0.6 212,261 - RR42_RS00875 0.57 -1.5 212,309 - RR42_RS00875 0.60 +0.3 212,447 - RR42_RS00875 0.68 +0.0 212,494 + RR42_RS00875 0.70 +0.2 212,494 + RR42_RS00875 0.70 -0.7 212,495 - RR42_RS00875 0.70 -1.1 212,495 - RR42_RS00875 0.70 +0.3 212,496 + RR42_RS00875 0.70 -1.6 212,496 + RR42_RS00875 0.70 -0.9 212,610 + RR42_RS00875 0.76 -1.7 212,632 + RR42_RS00875 0.78 -1.8 212,632 + RR42_RS00875 0.78 -0.5 212,632 + RR42_RS00875 0.78 -0.5 212,632 + RR42_RS00875 0.78 -0.3 212,632 + RR42_RS00875 0.78 -0.6 212,941 + -2.0 212,941 + -0.2 212,941 + -0.3 212,941 + +0.3 213,739 + -0.7 213,739 + -1.2 213,743 + -0.2 213,743 + +0.3 213,744 - -0.4 213,820 + +1.5 213,820 + +1.1
Or see this region's nucleotide sequence