Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS01825

Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS01810 and MMJJ_RS01815 are separated by 22 nucleotidesMMJJ_RS01815 and MMJJ_RS01820 are separated by 10 nucleotidesMMJJ_RS01820 and MMJJ_RS01825 are separated by 105 nucleotidesMMJJ_RS01825 and MMJJ_RS01830 are separated by 46 nucleotides MMJJ_RS01810: MMJJ_RS01810 - methyl coenzyme M reductase system, component A2, at 347,308 to 348,903 _RS01810 MMJJ_RS01815: MMJJ_RS01815 - DUF134 domain-containing protein, at 348,926 to 349,321 _RS01815 MMJJ_RS01820: MMJJ_RS01820 - MJ1244 family protein, at 349,332 to 349,661 _RS01820 MMJJ_RS01825: MMJJ_RS01825 - DNA repair and recombination protein RadB, at 349,767 to 350,417 _RS01825 MMJJ_RS01830: MMJJ_RS01830 - tRNA(Ile)(2)-agmatinylcytidine synthase, at 350,464 to 351,738 _RS01830 Position (kb) 349 350 351Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 348.850 kb on - strandat 349.022 kb on - strand, within MMJJ_RS01815at 349.027 kb on - strand, within MMJJ_RS01815at 349.046 kb on + strand, within MMJJ_RS01815at 349.065 kb on + strand, within MMJJ_RS01815at 349.211 kb on - strand, within MMJJ_RS01815at 349.252 kb on - strand, within MMJJ_RS01815at 349.256 kb on - strand, within MMJJ_RS01815at 349.333 kb on - strandat 349.333 kb on - strandat 349.382 kb on - strand, within MMJJ_RS01820at 349.382 kb on - strand, within MMJJ_RS01820at 349.382 kb on - strand, within MMJJ_RS01820at 349.382 kb on - strand, within MMJJ_RS01820at 349.383 kb on + strand, within MMJJ_RS01820at 349.383 kb on + strand, within MMJJ_RS01820at 349.383 kb on + strand, within MMJJ_RS01820at 349.383 kb on + strand, within MMJJ_RS01820at 349.480 kb on + strand, within MMJJ_RS01820at 349.677 kb on - strandat 349.677 kb on - strandat 349.677 kb on - strandat 349.696 kb on - strandat 349.696 kb on - strandat 349.696 kb on - strandat 349.712 kb on - strandat 349.714 kb on - strandat 349.714 kb on - strandat 349.714 kb on - strandat 349.714 kb on - strandat 349.715 kb on + strandat 349.715 kb on + strandat 349.715 kb on + strandat 349.715 kb on + strandat 349.715 kb on + strandat 349.716 kb on - strandat 349.718 kb on - strandat 349.719 kb on + strandat 349.751 kb on + strandat 349.781 kb on - strandat 349.809 kb on - strandat 349.863 kb on + strand, within MMJJ_RS01825at 349.863 kb on + strand, within MMJJ_RS01825at 349.921 kb on + strand, within MMJJ_RS01825at 349.954 kb on + strand, within MMJJ_RS01825at 349.954 kb on + strand, within MMJJ_RS01825at 349.976 kb on - strand, within MMJJ_RS01825at 350.055 kb on + strand, within MMJJ_RS01825at 350.093 kb on + strand, within MMJJ_RS01825at 350.120 kb on - strand, within MMJJ_RS01825at 350.120 kb on - strand, within MMJJ_RS01825at 350.121 kb on + strand, within MMJJ_RS01825at 350.121 kb on + strand, within MMJJ_RS01825at 350.211 kb on - strand, within MMJJ_RS01825at 350.334 kb on + strand, within MMJJ_RS01825at 350.373 kb on - strandat 350.417 kb on - strandat 350.418 kb on + strandat 350.444 kb on + strandat 350.449 kb on - strandat 350.461 kb on + strandat 350.461 kb on + strandat 350.465 kb on - strandat 350.692 kb on - strand, within MMJJ_RS01830at 350.803 kb on + strand, within MMJJ_RS01830at 350.928 kb on - strand, within MMJJ_RS01830at 351.034 kb on - strand, within MMJJ_RS01830at 351.151 kb on + strand, within MMJJ_RS01830at 351.248 kb on - strand, within MMJJ_RS01830at 351.253 kb on + strand, within MMJJ_RS01830at 351.400 kb on - strand, within MMJJ_RS01830

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
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348,850 - -2.4
349,022 - MMJJ_RS01815 0.24 -0.1
349,027 - MMJJ_RS01815 0.26 +0.4
349,046 + MMJJ_RS01815 0.30 -0.9
349,065 + MMJJ_RS01815 0.35 -1.1
349,211 - MMJJ_RS01815 0.72 -0.3
349,252 - MMJJ_RS01815 0.82 -0.3
349,256 - MMJJ_RS01815 0.83 -1.6
349,333 - -1.8
349,333 - -0.2
349,382 - MMJJ_RS01820 0.15 -0.8
349,382 - MMJJ_RS01820 0.15 -1.7
349,382 - MMJJ_RS01820 0.15 +0.6
349,382 - MMJJ_RS01820 0.15 -0.9
349,383 + MMJJ_RS01820 0.15 -2.8
349,383 + MMJJ_RS01820 0.15 -1.9
349,383 + MMJJ_RS01820 0.15 -1.5
349,383 + MMJJ_RS01820 0.15 -1.0
349,480 + MMJJ_RS01820 0.45 -0.4
349,677 - -1.9
349,677 - -0.5
349,677 - -0.1
349,696 - +2.3
349,696 - -2.2
349,696 - -1.1
349,712 - -0.1
349,714 - -0.4
349,714 - -0.3
349,714 - +0.9
349,714 - -0.7
349,715 + -1.5
349,715 + -0.2
349,715 + -0.5
349,715 + -0.5
349,715 + -0.1
349,716 - +0.3
349,718 - +0.5
349,719 + +0.3
349,751 + -0.8
349,781 - -0.4
349,809 - +1.3
349,863 + MMJJ_RS01825 0.15 -0.0
349,863 + MMJJ_RS01825 0.15 -0.3
349,921 + MMJJ_RS01825 0.24 -0.7
349,954 + MMJJ_RS01825 0.29 -0.3
349,954 + MMJJ_RS01825 0.29 +0.9
349,976 - MMJJ_RS01825 0.32 -0.1
350,055 + MMJJ_RS01825 0.44 -0.2
350,093 + MMJJ_RS01825 0.50 -0.2
350,120 - MMJJ_RS01825 0.54 -0.4
350,120 - MMJJ_RS01825 0.54 -0.1
350,121 + MMJJ_RS01825 0.54 +0.8
350,121 + MMJJ_RS01825 0.54 +0.5
350,211 - MMJJ_RS01825 0.68 +0.3
350,334 + MMJJ_RS01825 0.87 +0.6
350,373 - +0.3
350,417 - -0.6
350,418 + +0.6
350,444 + -1.1
350,449 - -0.3
350,461 + +0.9
350,461 + +0.3
350,465 - -0.8
350,692 - MMJJ_RS01830 0.18 -1.2
350,803 + MMJJ_RS01830 0.27 -2.4
350,928 - MMJJ_RS01830 0.36 -1.7
351,034 - MMJJ_RS01830 0.45 -1.9
351,151 + MMJJ_RS01830 0.54 -1.1
351,248 - MMJJ_RS01830 0.61 -1.1
351,253 + MMJJ_RS01830 0.62 -1.4
351,400 - MMJJ_RS01830 0.73 -2.8

Or see this region's nucleotide sequence