Experiment: R2A with Manganese (II) chloride tetrahydrate 3.125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS07410 and LRK54_RS07415 overlap by 4 nucleotides LRK54_RS07415 and LRK54_RS07420 overlap by 4 nucleotides LRK54_RS07420 and LRK54_RS07425 are separated by 1 nucleotides
LRK54_RS07410: LRK54_RS07410 - efflux RND transporter permease subunit, at 1,596,779 to 1,599,148
_RS07410
LRK54_RS07415: LRK54_RS07415 - YCF48-related protein, at 1,599,145 to 1,600,128
_RS07415
LRK54_RS07420: LRK54_RS07420 - hydroxymethylglutaryl-CoA lyase, at 1,600,125 to 1,601,051
_RS07420
LRK54_RS07425: LRK54_RS07425 - CoA transferase, at 1,601,053 to 1,602,252
_RS07425
Position (kb)
1599
1600
1601 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1598.232 kb on - strand, within LRK54_RS07410 at 1598.332 kb on - strand, within LRK54_RS07410 at 1598.348 kb on + strand, within LRK54_RS07410 at 1598.348 kb on + strand, within LRK54_RS07410 at 1598.349 kb on - strand, within LRK54_RS07410 at 1598.349 kb on - strand, within LRK54_RS07410 at 1598.487 kb on + strand, within LRK54_RS07410 at 1598.488 kb on - strand, within LRK54_RS07410 at 1598.542 kb on + strand, within LRK54_RS07410 at 1598.543 kb on - strand, within LRK54_RS07410 at 1598.543 kb on - strand, within LRK54_RS07410 at 1598.588 kb on + strand, within LRK54_RS07410 at 1598.588 kb on + strand, within LRK54_RS07410 at 1598.588 kb on + strand, within LRK54_RS07410 at 1598.598 kb on + strand, within LRK54_RS07410 at 1598.616 kb on + strand, within LRK54_RS07410 at 1598.616 kb on + strand, within LRK54_RS07410 at 1598.662 kb on - strand, within LRK54_RS07410 at 1598.662 kb on - strand, within LRK54_RS07410 at 1598.671 kb on - strand, within LRK54_RS07410 at 1598.711 kb on + strand, within LRK54_RS07410 at 1598.711 kb on + strand, within LRK54_RS07410 at 1598.712 kb on - strand, within LRK54_RS07410 at 1598.712 kb on - strand, within LRK54_RS07410 at 1598.924 kb on + strand at 1598.925 kb on - strand at 1598.925 kb on - strand at 1598.925 kb on - strand at 1598.961 kb on + strand at 1598.961 kb on + strand at 1598.961 kb on + strand at 1598.970 kb on + strand at 1598.971 kb on - strand at 1598.971 kb on - strand at 1598.971 kb on - strand at 1598.982 kb on + strand at 1598.982 kb on + strand at 1598.982 kb on + strand at 1598.982 kb on + strand at 1598.983 kb on - strand at 1598.983 kb on - strand at 1598.983 kb on - strand at 1598.983 kb on - strand at 1599.019 kb on + strand at 1599.019 kb on + strand at 1599.019 kb on + strand at 1599.020 kb on - strand at 1599.020 kb on - strand at 1599.137 kb on + strand at 1599.137 kb on + strand at 1599.138 kb on - strand at 1599.176 kb on + strand at 1599.176 kb on + strand at 1599.176 kb on + strand at 1599.177 kb on - strand at 1599.177 kb on - strand at 1599.254 kb on + strand, within LRK54_RS07415 at 1599.254 kb on + strand, within LRK54_RS07415 at 1599.254 kb on + strand, within LRK54_RS07415 at 1599.254 kb on + strand, within LRK54_RS07415 at 1599.254 kb on + strand, within LRK54_RS07415 at 1599.254 kb on + strand, within LRK54_RS07415 at 1599.254 kb on + strand, within LRK54_RS07415 at 1599.254 kb on + strand, within LRK54_RS07415 at 1599.254 kb on + strand, within LRK54_RS07415 at 1599.254 kb on + strand, within LRK54_RS07415 at 1599.254 kb on + strand, within LRK54_RS07415 at 1599.254 kb on + strand, within LRK54_RS07415 at 1599.254 kb on + strand, within LRK54_RS07415 at 1599.254 kb on + strand, within LRK54_RS07415 at 1599.255 kb on - strand, within LRK54_RS07415 at 1599.255 kb on - strand, within LRK54_RS07415 at 1599.255 kb on - strand, within LRK54_RS07415 at 1599.255 kb on - strand, within LRK54_RS07415 at 1599.255 kb on - strand, within LRK54_RS07415 at 1599.255 kb on - strand, within LRK54_RS07415 at 1599.255 kb on - strand, within LRK54_RS07415 at 1599.255 kb on - strand, within LRK54_RS07415 at 1599.257 kb on + strand, within LRK54_RS07415 at 1599.257 kb on + strand, within LRK54_RS07415 at 1599.257 kb on + strand, within LRK54_RS07415 at 1599.257 kb on + strand, within LRK54_RS07415 at 1599.258 kb on - strand, within LRK54_RS07415 at 1599.258 kb on - strand, within LRK54_RS07415 at 1599.382 kb on - strand, within LRK54_RS07415 at 1599.393 kb on + strand, within LRK54_RS07415 at 1599.440 kb on + strand, within LRK54_RS07415 at 1599.959 kb on + strand, within LRK54_RS07415 at 1599.959 kb on + strand, within LRK54_RS07415 at 1599.960 kb on - strand, within LRK54_RS07415 at 1600.052 kb on + strand at 1600.052 kb on + strand at 1600.052 kb on + strand at 1600.052 kb on + strand at 1600.052 kb on + strand at 1600.052 kb on + strand at 1600.052 kb on + strand at 1600.052 kb on + strand at 1600.052 kb on + strand at 1600.052 kb on + strand at 1600.052 kb on + strand at 1600.052 kb on + strand at 1600.052 kb on + strand at 1600.052 kb on + strand at 1600.052 kb on + strand at 1600.052 kb on + strand at 1600.053 kb on - strand at 1600.053 kb on - strand at 1600.053 kb on - strand at 1600.053 kb on - strand at 1600.053 kb on - strand at 1600.053 kb on - strand at 1600.053 kb on - strand at 1600.053 kb on - strand at 1600.053 kb on - strand at 1600.053 kb on - strand at 1600.053 kb on - strand at 1600.053 kb on - strand at 1600.053 kb on - strand at 1600.053 kb on - strand at 1600.053 kb on - strand at 1600.064 kb on + strand at 1600.398 kb on + strand, within LRK54_RS07420 at 1600.398 kb on + strand, within LRK54_RS07420 at 1600.398 kb on + strand, within LRK54_RS07420 at 1600.398 kb on + strand, within LRK54_RS07420 at 1600.398 kb on + strand, within LRK54_RS07420 at 1600.398 kb on + strand, within LRK54_RS07420 at 1600.398 kb on + strand, within LRK54_RS07420 at 1600.398 kb on + strand, within LRK54_RS07420 at 1600.398 kb on + strand, within LRK54_RS07420 at 1600.399 kb on - strand, within LRK54_RS07420 at 1600.399 kb on - strand, within LRK54_RS07420 at 1600.399 kb on - strand, within LRK54_RS07420 at 1600.399 kb on - strand, within LRK54_RS07420 at 1600.399 kb on - strand, within LRK54_RS07420 at 1600.399 kb on - strand, within LRK54_RS07420 at 1600.399 kb on - strand, within LRK54_RS07420 at 1600.399 kb on - strand, within LRK54_RS07420 at 1600.399 kb on - strand, within LRK54_RS07420 at 1600.399 kb on - strand, within LRK54_RS07420 at 1600.399 kb on - strand, within LRK54_RS07420 at 1600.399 kb on - strand, within LRK54_RS07420 at 1600.399 kb on - strand, within LRK54_RS07420 at 1600.399 kb on - strand, within LRK54_RS07420 at 1600.399 kb on - strand, within LRK54_RS07420 at 1600.399 kb on - strand, within LRK54_RS07420 at 1600.399 kb on - strand, within LRK54_RS07420 at 1600.399 kb on - strand, within LRK54_RS07420 at 1600.399 kb on - strand, within LRK54_RS07420 at 1600.400 kb on + strand, within LRK54_RS07420 at 1600.400 kb on + strand, within LRK54_RS07420 at 1600.400 kb on + strand, within LRK54_RS07420 at 1600.400 kb on + strand, within LRK54_RS07420 at 1600.400 kb on + strand, within LRK54_RS07420 at 1600.400 kb on + strand, within LRK54_RS07420 at 1600.401 kb on - strand, within LRK54_RS07420 at 1600.401 kb on - strand, within LRK54_RS07420 at 1600.401 kb on - strand, within LRK54_RS07420 at 1600.401 kb on - strand, within LRK54_RS07420 at 1600.401 kb on - strand, within LRK54_RS07420 at 1600.401 kb on - strand, within LRK54_RS07420 at 1600.606 kb on + strand, within LRK54_RS07420 at 1600.607 kb on - strand, within LRK54_RS07420 at 1600.607 kb on - strand, within LRK54_RS07420 at 1600.607 kb on - strand, within LRK54_RS07420 at 1600.607 kb on - strand, within LRK54_RS07420 at 1600.607 kb on - strand, within LRK54_RS07420 at 1600.607 kb on - strand, within LRK54_RS07420 at 1600.607 kb on - strand, within LRK54_RS07420 at 1600.618 kb on + strand, within LRK54_RS07420 at 1600.618 kb on + strand, within LRK54_RS07420 at 1600.618 kb on + strand, within LRK54_RS07420 at 1600.619 kb on - strand, within LRK54_RS07420 at 1600.619 kb on - strand, within LRK54_RS07420 at 1600.619 kb on - strand, within LRK54_RS07420 at 1600.633 kb on + strand, within LRK54_RS07420 at 1600.633 kb on + strand, within LRK54_RS07420 at 1600.633 kb on + strand, within LRK54_RS07420 at 1600.633 kb on + strand, within LRK54_RS07420 at 1600.633 kb on + strand, within LRK54_RS07420 at 1600.634 kb on - strand, within LRK54_RS07420 at 1600.634 kb on - strand, within LRK54_RS07420 at 1600.634 kb on - strand, within LRK54_RS07420 at 1600.634 kb on - strand, within LRK54_RS07420 at 1600.634 kb on - strand, within LRK54_RS07420 at 1600.634 kb on - strand, within LRK54_RS07420 at 1600.796 kb on - strand, within LRK54_RS07420 at 1600.796 kb on - strand, within LRK54_RS07420 at 1600.825 kb on + strand, within LRK54_RS07420 at 1600.825 kb on + strand, within LRK54_RS07420 at 1600.825 kb on + strand, within LRK54_RS07420 at 1600.826 kb on - strand, within LRK54_RS07420 at 1600.826 kb on - strand, within LRK54_RS07420 at 1600.826 kb on - strand, within LRK54_RS07420 at 1600.984 kb on - strand at 1601.034 kb on + strand at 1601.034 kb on + strand at 1601.034 kb on + strand at 1601.034 kb on + strand at 1601.034 kb on + strand at 1601.034 kb on + strand at 1601.035 kb on - strand at 1601.035 kb on - strand at 1601.035 kb on - strand at 1601.035 kb on - strand at 1601.035 kb on - strand at 1601.035 kb on - strand at 1601.035 kb on - strand at 1601.035 kb on - strand at 1601.102 kb on + strand at 1601.103 kb on - strand at 1601.122 kb on + strand at 1601.122 kb on + strand at 1601.122 kb on + strand at 1601.122 kb on + strand at 1601.123 kb on - strand at 1601.123 kb on - strand at 1601.123 kb on - strand at 1601.123 kb on - strand at 1601.123 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Manganese (II) chloride tetrahydrate 3.125 mM remove 1,598,232 - LRK54_RS07410 0.61 +2.9 1,598,332 - LRK54_RS07410 0.66 -0.6 1,598,348 + LRK54_RS07410 0.66 -0.7 1,598,348 + LRK54_RS07410 0.66 -0.6 1,598,349 - LRK54_RS07410 0.66 -0.4 1,598,349 - LRK54_RS07410 0.66 +0.3 1,598,487 + LRK54_RS07410 0.72 +1.0 1,598,488 - LRK54_RS07410 0.72 -0.4 1,598,542 + LRK54_RS07410 0.74 -0.4 1,598,543 - LRK54_RS07410 0.74 -0.1 1,598,543 - LRK54_RS07410 0.74 -0.2 1,598,588 + LRK54_RS07410 0.76 -0.9 1,598,588 + LRK54_RS07410 0.76 -0.9 1,598,588 + LRK54_RS07410 0.76 -1.9 1,598,598 + LRK54_RS07410 0.77 -1.0 1,598,616 + LRK54_RS07410 0.78 -1.2 1,598,616 + LRK54_RS07410 0.78 -0.9 1,598,662 - LRK54_RS07410 0.79 -0.0 1,598,662 - LRK54_RS07410 0.79 +0.8 1,598,671 - LRK54_RS07410 0.80 +0.3 1,598,711 + LRK54_RS07410 0.82 -1.9 1,598,711 + LRK54_RS07410 0.82 -0.1 1,598,712 - LRK54_RS07410 0.82 +0.6 1,598,712 - LRK54_RS07410 0.82 -0.3 1,598,924 + -1.2 1,598,925 - -0.4 1,598,925 - -0.1 1,598,925 - +0.3 1,598,961 + +0.1 1,598,961 + -0.1 1,598,961 + +0.4 1,598,970 + +0.4 1,598,971 - +0.2 1,598,971 - +0.6 1,598,971 - -1.8 1,598,982 + +0.4 1,598,982 + -0.3 1,598,982 + +0.9 1,598,982 + +0.4 1,598,983 - +0.6 1,598,983 - -0.0 1,598,983 - -1.0 1,598,983 - +1.4 1,599,019 + +0.3 1,599,019 + -0.9 1,599,019 + -0.4 1,599,020 - -0.9 1,599,020 - -0.8 1,599,137 + -1.0 1,599,137 + -0.3 1,599,138 - -0.6 1,599,176 + -0.6 1,599,176 + +1.4 1,599,176 + -1.0 1,599,177 - +0.2 1,599,177 - -0.6 1,599,254 + LRK54_RS07415 0.11 -0.7 1,599,254 + LRK54_RS07415 0.11 +0.7 1,599,254 + LRK54_RS07415 0.11 -1.9 1,599,254 + LRK54_RS07415 0.11 -0.6 1,599,254 + LRK54_RS07415 0.11 -1.1 1,599,254 + LRK54_RS07415 0.11 -0.3 1,599,254 + LRK54_RS07415 0.11 +1.8 1,599,254 + LRK54_RS07415 0.11 -0.6 1,599,254 + LRK54_RS07415 0.11 +0.1 1,599,254 + LRK54_RS07415 0.11 -2.2 1,599,254 + LRK54_RS07415 0.11 -0.9 1,599,254 + LRK54_RS07415 0.11 +1.6 1,599,254 + LRK54_RS07415 0.11 +2.4 1,599,254 + LRK54_RS07415 0.11 +0.0 1,599,255 - LRK54_RS07415 0.11 -0.3 1,599,255 - LRK54_RS07415 0.11 +1.1 1,599,255 - LRK54_RS07415 0.11 -1.8 1,599,255 - LRK54_RS07415 0.11 -0.5 1,599,255 - LRK54_RS07415 0.11 -2.6 1,599,255 - LRK54_RS07415 0.11 -0.7 1,599,255 - LRK54_RS07415 0.11 -0.4 1,599,255 - LRK54_RS07415 0.11 -0.9 1,599,257 + LRK54_RS07415 0.11 +0.4 1,599,257 + LRK54_RS07415 0.11 -1.1 1,599,257 + LRK54_RS07415 0.11 -2.0 1,599,257 + LRK54_RS07415 0.11 -0.7 1,599,258 - LRK54_RS07415 0.11 -1.0 1,599,258 - LRK54_RS07415 0.11 -0.3 1,599,382 - LRK54_RS07415 0.24 +1.0 1,599,393 + LRK54_RS07415 0.25 +1.0 1,599,440 + LRK54_RS07415 0.30 -0.2 1,599,959 + LRK54_RS07415 0.83 +0.7 1,599,959 + LRK54_RS07415 0.83 -0.2 1,599,960 - LRK54_RS07415 0.83 +0.2 1,600,052 + +0.1 1,600,052 + -0.4 1,600,052 + -0.5 1,600,052 + -0.1 1,600,052 + +0.0 1,600,052 + +2.9 1,600,052 + -1.0 1,600,052 + +0.0 1,600,052 + +0.0 1,600,052 + -1.0 1,600,052 + -1.0 1,600,052 + -0.3 1,600,052 + -1.2 1,600,052 + +0.6 1,600,052 + -0.8 1,600,052 + +0.1 1,600,053 - -0.8 1,600,053 - -0.8 1,600,053 - -0.1 1,600,053 - -0.6 1,600,053 - +1.4 1,600,053 - -0.8 1,600,053 - -2.4 1,600,053 - -0.4 1,600,053 - -0.6 1,600,053 - -1.4 1,600,053 - -0.6 1,600,053 - -0.7 1,600,053 - -0.2 1,600,053 - +0.3 1,600,053 - +0.0 1,600,064 + +0.1 1,600,398 + LRK54_RS07420 0.29 +0.4 1,600,398 + LRK54_RS07420 0.29 +0.5 1,600,398 + LRK54_RS07420 0.29 -0.4 1,600,398 + LRK54_RS07420 0.29 -0.9 1,600,398 + LRK54_RS07420 0.29 +0.2 1,600,398 + LRK54_RS07420 0.29 -0.5 1,600,398 + LRK54_RS07420 0.29 -0.4 1,600,398 + LRK54_RS07420 0.29 +0.1 1,600,398 + LRK54_RS07420 0.29 -0.4 1,600,399 - LRK54_RS07420 0.30 -0.4 1,600,399 - LRK54_RS07420 0.30 +0.6 1,600,399 - LRK54_RS07420 0.30 +0.0 1,600,399 - LRK54_RS07420 0.30 +0.4 1,600,399 - LRK54_RS07420 0.30 +0.4 1,600,399 - LRK54_RS07420 0.30 -0.4 1,600,399 - LRK54_RS07420 0.30 +0.1 1,600,399 - LRK54_RS07420 0.30 -0.7 1,600,399 - LRK54_RS07420 0.30 -0.9 1,600,399 - LRK54_RS07420 0.30 +0.7 1,600,399 - LRK54_RS07420 0.30 -2.2 1,600,399 - LRK54_RS07420 0.30 +1.4 1,600,399 - LRK54_RS07420 0.30 -0.3 1,600,399 - LRK54_RS07420 0.30 +0.8 1,600,399 - LRK54_RS07420 0.30 +1.7 1,600,399 - LRK54_RS07420 0.30 -1.0 1,600,399 - LRK54_RS07420 0.30 -1.1 1,600,399 - LRK54_RS07420 0.30 -0.0 1,600,399 - LRK54_RS07420 0.30 -2.8 1,600,400 + LRK54_RS07420 0.30 -0.1 1,600,400 + LRK54_RS07420 0.30 -0.1 1,600,400 + LRK54_RS07420 0.30 +1.6 1,600,400 + LRK54_RS07420 0.30 -0.5 1,600,400 + LRK54_RS07420 0.30 -0.1 1,600,400 + LRK54_RS07420 0.30 -2.1 1,600,401 - LRK54_RS07420 0.30 +0.2 1,600,401 - LRK54_RS07420 0.30 +0.5 1,600,401 - LRK54_RS07420 0.30 -0.5 1,600,401 - LRK54_RS07420 0.30 +0.8 1,600,401 - LRK54_RS07420 0.30 +1.0 1,600,401 - LRK54_RS07420 0.30 +0.5 1,600,606 + LRK54_RS07420 0.52 -0.4 1,600,607 - LRK54_RS07420 0.52 +0.5 1,600,607 - LRK54_RS07420 0.52 +1.4 1,600,607 - LRK54_RS07420 0.52 -0.8 1,600,607 - LRK54_RS07420 0.52 -0.1 1,600,607 - LRK54_RS07420 0.52 -0.6 1,600,607 - LRK54_RS07420 0.52 -0.4 1,600,607 - LRK54_RS07420 0.52 +0.6 1,600,618 + LRK54_RS07420 0.53 -1.7 1,600,618 + LRK54_RS07420 0.53 +0.4 1,600,618 + LRK54_RS07420 0.53 -1.0 1,600,619 - LRK54_RS07420 0.53 +0.0 1,600,619 - LRK54_RS07420 0.53 -1.6 1,600,619 - LRK54_RS07420 0.53 +0.9 1,600,633 + LRK54_RS07420 0.55 -0.2 1,600,633 + LRK54_RS07420 0.55 -0.2 1,600,633 + LRK54_RS07420 0.55 -1.2 1,600,633 + LRK54_RS07420 0.55 +1.2 1,600,633 + LRK54_RS07420 0.55 -0.8 1,600,634 - LRK54_RS07420 0.55 -0.1 1,600,634 - LRK54_RS07420 0.55 -0.3 1,600,634 - LRK54_RS07420 0.55 -0.7 1,600,634 - LRK54_RS07420 0.55 -1.0 1,600,634 - LRK54_RS07420 0.55 -0.3 1,600,634 - LRK54_RS07420 0.55 -0.1 1,600,796 - LRK54_RS07420 0.72 -0.4 1,600,796 - LRK54_RS07420 0.72 +0.2 1,600,825 + LRK54_RS07420 0.76 -0.6 1,600,825 + LRK54_RS07420 0.76 +0.4 1,600,825 + LRK54_RS07420 0.76 -0.4 1,600,826 - LRK54_RS07420 0.76 -1.3 1,600,826 - LRK54_RS07420 0.76 -0.2 1,600,826 - LRK54_RS07420 0.76 +0.3 1,600,984 - +0.0 1,601,034 + -0.7 1,601,034 + -0.3 1,601,034 + -0.1 1,601,034 + -0.7 1,601,034 + -0.4 1,601,034 + +0.5 1,601,035 - +0.4 1,601,035 - -2.3 1,601,035 - -0.2 1,601,035 - -1.5 1,601,035 - -0.2 1,601,035 - +0.2 1,601,035 - +0.4 1,601,035 - +0.4 1,601,102 + +0.1 1,601,103 - -1.1 1,601,122 + -0.7 1,601,122 + -0.6 1,601,122 + +1.0 1,601,122 + +0.0 1,601,123 - +0.6 1,601,123 - +0.9 1,601,123 - +0.2 1,601,123 - -1.1 1,601,123 - -0.4
Or see this region's nucleotide sequence