Experiment: Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14215 and BPHYT_RS14220 overlap by 4 nucleotides BPHYT_RS14220 and BPHYT_RS14225 are separated by 80 nucleotides BPHYT_RS14225 and BPHYT_RS14230 are separated by 48 nucleotides BPHYT_RS14230 and BPHYT_RS14235 are separated by 512 nucleotides
BPHYT_RS14215: BPHYT_RS14215 - SAM-dependent methyltransferase, at 3,248,932 to 3,249,795
_RS14215
BPHYT_RS14220: BPHYT_RS14220 - ribonuclease H, at 3,249,792 to 3,250,238
_RS14220
BPHYT_RS14225: BPHYT_RS14225 - DNA polymerase III subunit epsilon, at 3,250,319 to 3,251,053
_RS14225
BPHYT_RS14230: BPHYT_RS14230 - tRNA-Val, at 3,251,102 to 3,251,176
_RS14230
BPHYT_RS14235: BPHYT_RS14235 - multidrug ABC transporter ATPase, at 3,251,689 to 3,253,566
_RS14235
Position (kb)
3250
3251
3252 Strain fitness (log2 ratio)
-1
0
1 at 3249.774 kb on + strand at 3250.236 kb on + strand at 3250.236 kb on + strand at 3250.236 kb on + strand at 3250.237 kb on - strand at 3250.295 kb on + strand at 3250.295 kb on + strand at 3251.058 kb on + strand at 3251.259 kb on + strand at 3251.259 kb on + strand at 3251.260 kb on - strand at 3251.343 kb on + strand at 3251.371 kb on + strand at 3251.372 kb on - strand at 3251.372 kb on - strand at 3251.374 kb on - strand at 3251.374 kb on - strand at 3251.374 kb on - strand at 3251.374 kb on - strand at 3251.374 kb on - strand at 3251.495 kb on + strand at 3251.495 kb on + strand at 3251.495 kb on + strand at 3251.603 kb on + strand at 3251.746 kb on - strand at 3251.971 kb on + strand, within BPHYT_RS14235 at 3251.971 kb on + strand, within BPHYT_RS14235 at 3251.972 kb on - strand, within BPHYT_RS14235
Per-strain Table
Position Strand Gene LocusTag Fraction Lomefloxacin 0.001 mM remove 3,249,774 + +0.1 3,250,236 + +0.7 3,250,236 + +0.1 3,250,236 + -0.0 3,250,237 - -0.1 3,250,295 + -1.1 3,250,295 + -0.4 3,251,058 + -0.3 3,251,259 + -1.3 3,251,259 + -0.1 3,251,260 - +0.0 3,251,343 + +0.2 3,251,371 + -0.2 3,251,372 - -0.4 3,251,372 - -0.3 3,251,374 - +0.5 3,251,374 - -0.3 3,251,374 - -0.6 3,251,374 - -0.2 3,251,374 - -0.0 3,251,495 + +0.8 3,251,495 + +0.3 3,251,495 + -0.4 3,251,603 + +0.3 3,251,746 - -0.5 3,251,971 + BPHYT_RS14235 0.15 +1.3 3,251,971 + BPHYT_RS14235 0.15 -0.4 3,251,972 - BPHYT_RS14235 0.15 +0.5
Or see this region's nucleotide sequence