Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS14225

Experiment: Lomefloxacin 0.001 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS14215 and BPHYT_RS14220 overlap by 4 nucleotidesBPHYT_RS14220 and BPHYT_RS14225 are separated by 80 nucleotidesBPHYT_RS14225 and BPHYT_RS14230 are separated by 48 nucleotidesBPHYT_RS14230 and BPHYT_RS14235 are separated by 512 nucleotides BPHYT_RS14215: BPHYT_RS14215 - SAM-dependent methyltransferase, at 3,248,932 to 3,249,795 _RS14215 BPHYT_RS14220: BPHYT_RS14220 - ribonuclease H, at 3,249,792 to 3,250,238 _RS14220 BPHYT_RS14225: BPHYT_RS14225 - DNA polymerase III subunit epsilon, at 3,250,319 to 3,251,053 _RS14225 BPHYT_RS14230: BPHYT_RS14230 - tRNA-Val, at 3,251,102 to 3,251,176 _RS14230 BPHYT_RS14235: BPHYT_RS14235 - multidrug ABC transporter ATPase, at 3,251,689 to 3,253,566 _RS14235 Position (kb) 3250 3251 3252Strain fitness (log2 ratio) -1 0 1at 3249.774 kb on + strandat 3250.236 kb on + strandat 3250.236 kb on + strandat 3250.236 kb on + strandat 3250.237 kb on - strandat 3250.295 kb on + strandat 3250.295 kb on + strandat 3251.058 kb on + strandat 3251.259 kb on + strandat 3251.259 kb on + strandat 3251.260 kb on - strandat 3251.343 kb on + strandat 3251.371 kb on + strandat 3251.372 kb on - strandat 3251.372 kb on - strandat 3251.374 kb on - strandat 3251.374 kb on - strandat 3251.374 kb on - strandat 3251.374 kb on - strandat 3251.374 kb on - strandat 3251.495 kb on + strandat 3251.495 kb on + strandat 3251.495 kb on + strandat 3251.603 kb on + strandat 3251.746 kb on - strandat 3251.971 kb on + strand, within BPHYT_RS14235at 3251.971 kb on + strand, within BPHYT_RS14235at 3251.972 kb on - strand, within BPHYT_RS14235

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Per-strain Table

Position Strand Gene LocusTag Fraction Lomefloxacin 0.001 mM
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3,249,774 + +0.1
3,250,236 + +0.7
3,250,236 + +0.1
3,250,236 + -0.0
3,250,237 - -0.1
3,250,295 + -1.1
3,250,295 + -0.4
3,251,058 + -0.3
3,251,259 + -1.3
3,251,259 + -0.1
3,251,260 - +0.0
3,251,343 + +0.2
3,251,371 + -0.2
3,251,372 - -0.4
3,251,372 - -0.3
3,251,374 - +0.5
3,251,374 - -0.3
3,251,374 - -0.6
3,251,374 - -0.2
3,251,374 - -0.0
3,251,495 + +0.8
3,251,495 + +0.3
3,251,495 + -0.4
3,251,603 + +0.3
3,251,746 - -0.5
3,251,971 + BPHYT_RS14235 0.15 +1.3
3,251,971 + BPHYT_RS14235 0.15 -0.4
3,251,972 - BPHYT_RS14235 0.15 +0.5

Or see this region's nucleotide sequence