Experiment: Lomefloxacin 0.001 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS08850 and BPHYT_RS08855 are separated by 293 nucleotides BPHYT_RS08855 and BPHYT_RS08860 are separated by 51 nucleotides BPHYT_RS08860 and BPHYT_RS08865 are separated by 2 nucleotides BPHYT_RS08865 and BPHYT_RS08870 are separated by 27 nucleotides BPHYT_RS08870 and BPHYT_RS08875 are separated by 144 nucleotides
BPHYT_RS08850: BPHYT_RS08850 - transcriptional regulator, at 2,005,634 to 2,006,569
_RS08850
BPHYT_RS08855: BPHYT_RS08855 - 30S ribosomal protein S6, at 2,006,863 to 2,007,237
_RS08855
BPHYT_RS08860: BPHYT_RS08860 - primosomal replication protein N, at 2,007,289 to 2,007,588
_RS08860
BPHYT_RS08865: BPHYT_RS08865 - 30S ribosomal protein S18, at 2,007,591 to 2,007,866
_RS08865
BPHYT_RS08870: BPHYT_RS08870 - 50S ribosomal protein L9, at 2,007,894 to 2,008,346
_RS08870
BPHYT_RS08875: BPHYT_RS08875 - DNA helicase, at 2,008,491 to 2,009,876
_RS08875
Position (kb)
2007
2008 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2006.307 kb on - strand, within BPHYT_RS08850 at 2006.369 kb on + strand, within BPHYT_RS08850 at 2006.370 kb on - strand, within BPHYT_RS08850 at 2006.624 kb on + strand at 2006.625 kb on - strand at 2008.367 kb on + strand at 2008.368 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Lomefloxacin 0.001 mM remove 2,006,307 - BPHYT_RS08850 0.72 -2.4 2,006,369 + BPHYT_RS08850 0.79 -0.6 2,006,370 - BPHYT_RS08850 0.79 -2.1 2,006,624 + +0.6 2,006,625 - +0.7 2,008,367 + +4.0 2,008,368 - -0.5
Or see this region's nucleotide sequence