Experiment: Cephalothin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25060 and BPHYT_RS25065 are separated by 511 nucleotides BPHYT_RS25065 and BPHYT_RS25070 overlap by 8 nucleotides BPHYT_RS25070 and BPHYT_RS25075 are separated by 7 nucleotides
BPHYT_RS25060: BPHYT_RS25060 - membrane protein, at 1,192,558 to 1,193,688
_RS25060
BPHYT_RS25065: BPHYT_RS25065 - glycine/betaine ABC transporter ATPase, at 1,194,200 to 1,195,378
_RS25065
BPHYT_RS25070: BPHYT_RS25070 - choline ABC transporter permease, at 1,195,371 to 1,196,273
_RS25070
BPHYT_RS25075: BPHYT_RS25075 - AraC family transcriptional regulator, at 1,196,281 to 1,197,279
_RS25075
Position (kb)
1194
1195
1196 Strain fitness (log2 ratio)
-2
-1
0
1 at 1193.480 kb on + strand, within BPHYT_RS25060 at 1193.506 kb on + strand, within BPHYT_RS25060 at 1193.708 kb on + strand at 1193.999 kb on + strand at 1194.000 kb on - strand at 1194.003 kb on + strand at 1194.003 kb on + strand at 1194.004 kb on - strand at 1194.004 kb on - strand at 1194.004 kb on - strand at 1194.004 kb on - strand at 1194.004 kb on - strand at 1194.042 kb on - strand at 1194.042 kb on - strand at 1194.042 kb on - strand at 1194.156 kb on + strand at 1194.157 kb on - strand at 1194.187 kb on - strand at 1195.171 kb on - strand, within BPHYT_RS25065 at 1195.310 kb on + strand at 1195.311 kb on - strand at 1195.608 kb on + strand, within BPHYT_RS25070 at 1196.228 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 2 mg/ml remove 1,193,480 + BPHYT_RS25060 0.82 +0.0 1,193,506 + BPHYT_RS25060 0.84 +0.7 1,193,708 + +0.3 1,193,999 + +0.5 1,194,000 - +0.9 1,194,003 + +1.2 1,194,003 + +0.1 1,194,004 - -0.3 1,194,004 - -2.1 1,194,004 - +0.2 1,194,004 - +0.6 1,194,004 - -0.2 1,194,042 - -0.2 1,194,042 - -2.5 1,194,042 - +0.7 1,194,156 + -0.4 1,194,157 - +0.4 1,194,187 - +0.2 1,195,171 - BPHYT_RS25065 0.82 -0.8 1,195,310 + -0.3 1,195,311 - +0.4 1,195,608 + BPHYT_RS25070 0.26 +0.3 1,196,228 + -0.0
Or see this region's nucleotide sequence