Experiment: Cephalothin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10955 and BPHYT_RS10960 are separated by 592 nucleotides BPHYT_RS10960 and BPHYT_RS10965 are separated by 53 nucleotides BPHYT_RS10965 and BPHYT_RS10970 are separated by 78 nucleotides BPHYT_RS10970 and BPHYT_RS10975 are separated by 57 nucleotides BPHYT_RS10975 and BPHYT_RS10980 are separated by 328 nucleotides
BPHYT_RS10955: BPHYT_RS10955 - dioxygenase, at 2,492,643 to 2,493,575
_RS10955
BPHYT_RS10960: BPHYT_RS10960 - hypothetical protein, at 2,494,168 to 2,494,281
_RS10960
BPHYT_RS10965: BPHYT_RS10965 - hypothetical protein, at 2,494,335 to 2,494,724
_RS10965
BPHYT_RS10970: BPHYT_RS10970 - hypothetical protein, at 2,494,803 to 2,495,051
_RS10970
BPHYT_RS10975: BPHYT_RS10975 - hypothetical protein, at 2,495,109 to 2,495,351
_RS10975
BPHYT_RS10980: BPHYT_RS10980 - 2-dehydro-3-deoxy-6-phosphogalactonate aldolase, at 2,495,680 to 2,496,927
_RS10980
Position (kb)
2494
2495 Strain fitness (log2 ratio)
-1
0
1 at 2493.579 kb on - strand at 2493.579 kb on - strand at 2494.121 kb on - strand at 2494.133 kb on + strand at 2494.317 kb on - strand at 2494.557 kb on + strand, within BPHYT_RS10965 at 2495.160 kb on - strand, within BPHYT_RS10975 at 2495.482 kb on + strand at 2495.483 kb on - strand at 2495.662 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 2 mg/ml remove 2,493,579 - -1.2 2,493,579 - +0.2 2,494,121 - -0.1 2,494,133 + +0.1 2,494,317 - +0.1 2,494,557 + BPHYT_RS10965 0.57 -0.6 2,495,160 - BPHYT_RS10975 0.21 -0.5 2,495,482 + +0.7 2,495,483 - +0.9 2,495,662 - +0.8
Or see this region's nucleotide sequence