Experiment: Cephalothin 2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02275 and BPHYT_RS02280 are separated by 15 nucleotides BPHYT_RS02280 and BPHYT_RS02285 are separated by 172 nucleotides BPHYT_RS02285 and BPHYT_RS02290 are separated by 61 nucleotides
BPHYT_RS02275: BPHYT_RS02275 - short-chain dehydrogenase, at 521,579 to 522,358
_RS02275
BPHYT_RS02280: BPHYT_RS02280 - DSBA oxidoreductase, at 522,374 to 523,012
_RS02280
BPHYT_RS02285: BPHYT_RS02285 - cell division protein, at 523,185 to 524,009
_RS02285
BPHYT_RS02290: BPHYT_RS02290 - arginine--tRNA ligase, at 524,071 to 525,861
_RS02290
Position (kb) 523
524
525 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 522.192 kb on + strand, within BPHYT_RS02275 at 522.193 kb on - strand, within BPHYT_RS02275 at 522.193 kb on - strand, within BPHYT_RS02275 at 522.311 kb on + strand at 522.311 kb on + strand at 522.312 kb on - strand at 522.312 kb on - strand at 522.375 kb on + strand at 523.029 kb on + strand at 523.030 kb on - strand at 523.030 kb on - strand at 523.136 kb on - strand at 523.171 kb on + strand at 523.171 kb on + strand at 523.172 kb on - strand at 523.172 kb on - strand at 523.172 kb on - strand at 523.175 kb on + strand at 523.176 kb on - strand at 523.176 kb on - strand at 523.187 kb on - strand at 523.187 kb on - strand at 523.294 kb on + strand, within BPHYT_RS02285 at 523.297 kb on + strand, within BPHYT_RS02285 at 523.297 kb on + strand, within BPHYT_RS02285 at 523.297 kb on + strand, within BPHYT_RS02285 at 523.298 kb on - strand, within BPHYT_RS02285 at 523.879 kb on + strand, within BPHYT_RS02285 at 523.879 kb on + strand, within BPHYT_RS02285 at 524.072 kb on + strand at 524.072 kb on + strand at 524.072 kb on + strand at 524.073 kb on - strand at 524.073 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 2 mg/ml remove 522,192 + BPHYT_RS02275 0.79 -0.2 522,193 - BPHYT_RS02275 0.79 -2.0 522,193 - BPHYT_RS02275 0.79 +0.2 522,311 + +0.4 522,311 + +0.8 522,312 - +0.3 522,312 - -0.4 522,375 + -1.2 523,029 + -2.6 523,030 - -0.0 523,030 - -0.5 523,136 - +0.1 523,171 + -1.1 523,171 + -0.0 523,172 - +0.5 523,172 - +0.0 523,172 - +1.5 523,175 + -0.9 523,176 - -0.0 523,176 - -0.7 523,187 - -0.4 523,187 - -0.2 523,294 + BPHYT_RS02285 0.13 -1.5 523,297 + BPHYT_RS02285 0.14 +0.5 523,297 + BPHYT_RS02285 0.14 -1.7 523,297 + BPHYT_RS02285 0.14 -1.4 523,298 - BPHYT_RS02285 0.14 -2.3 523,879 + BPHYT_RS02285 0.84 -4.1 523,879 + BPHYT_RS02285 0.84 -2.7 524,072 + -2.6 524,072 + -1.7 524,072 + -1.2 524,073 - -0.8 524,073 - +0.2
Or see this region's nucleotide sequence