Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22960 and RR42_RS22965 are separated by 45 nucleotides RR42_RS22965 and RR42_RS22970 overlap by 4 nucleotides RR42_RS22970 and RR42_RS22975 are separated by 143 nucleotides
RR42_RS22960: RR42_RS22960 - alpha/beta hydrolase, at 484,876 to 485,757
_RS22960
RR42_RS22965: RR42_RS22965 - 3-oxoadipate CoA-transferase, at 485,803 to 486,438
_RS22965
RR42_RS22970: RR42_RS22970 - 3-oxoadipate CoA-transferase, at 486,435 to 487,127
_RS22970
RR42_RS22975: RR42_RS22975 - LysR family transcriptional regulator, at 487,271 to 488,185
_RS22975
Position (kb) 486
487
488 Strain fitness (log2 ratio) -1
0
1 at 485.517 kb on + strand, within RR42_RS22960 at 485.518 kb on - strand, within RR42_RS22960 at 485.551 kb on + strand, within RR42_RS22960 at 485.551 kb on + strand, within RR42_RS22960 at 485.551 kb on + strand, within RR42_RS22960 at 485.551 kb on + strand, within RR42_RS22960 at 485.552 kb on - strand, within RR42_RS22960 at 485.552 kb on - strand, within RR42_RS22960 at 485.552 kb on - strand, within RR42_RS22960 at 485.810 kb on - strand at 485.916 kb on + strand, within RR42_RS22965 at 485.919 kb on - strand, within RR42_RS22965 at 485.919 kb on - strand, within RR42_RS22965 at 486.122 kb on + strand, within RR42_RS22965 at 486.300 kb on + strand, within RR42_RS22965 at 486.374 kb on + strand, within RR42_RS22965 at 486.619 kb on + strand, within RR42_RS22970 at 486.766 kb on + strand, within RR42_RS22970 at 486.766 kb on + strand, within RR42_RS22970 at 486.767 kb on - strand, within RR42_RS22970 at 486.767 kb on - strand, within RR42_RS22970 at 486.807 kb on + strand, within RR42_RS22970 at 486.807 kb on + strand, within RR42_RS22970 at 486.807 kb on + strand, within RR42_RS22970 at 486.808 kb on - strand, within RR42_RS22970 at 486.808 kb on - strand, within RR42_RS22970 at 487.220 kb on + strand at 487.220 kb on + strand at 487.227 kb on + strand at 487.245 kb on - strand at 487.245 kb on - strand at 487.293 kb on - strand at 487.357 kb on - strand at 487.357 kb on - strand at 487.357 kb on - strand at 487.565 kb on + strand, within RR42_RS22975 at 487.565 kb on + strand, within RR42_RS22975 at 487.566 kb on - strand, within RR42_RS22975 at 487.566 kb on - strand, within RR42_RS22975 at 487.593 kb on + strand, within RR42_RS22975 at 487.593 kb on + strand, within RR42_RS22975 at 487.594 kb on - strand, within RR42_RS22975 at 487.859 kb on - strand, within RR42_RS22975 at 487.892 kb on + strand, within RR42_RS22975 at 487.892 kb on + strand, within RR42_RS22975 at 487.892 kb on + strand, within RR42_RS22975 at 487.893 kb on - strand, within RR42_RS22975 at 487.893 kb on - strand, within RR42_RS22975 at 488.050 kb on - strand, within RR42_RS22975 at 488.050 kb on - strand, within RR42_RS22975 at 488.050 kb on - strand, within RR42_RS22975 at 488.050 kb on - strand, within RR42_RS22975
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 485,517 + RR42_RS22960 0.73 -0.5 485,518 - RR42_RS22960 0.73 -0.1 485,551 + RR42_RS22960 0.77 -0.3 485,551 + RR42_RS22960 0.77 +0.2 485,551 + RR42_RS22960 0.77 -0.2 485,551 + RR42_RS22960 0.77 +0.3 485,552 - RR42_RS22960 0.77 +0.8 485,552 - RR42_RS22960 0.77 -0.6 485,552 - RR42_RS22960 0.77 +0.4 485,810 - +0.3 485,916 + RR42_RS22965 0.18 -0.3 485,919 - RR42_RS22965 0.18 +0.5 485,919 - RR42_RS22965 0.18 -0.3 486,122 + RR42_RS22965 0.50 -0.7 486,300 + RR42_RS22965 0.78 +1.0 486,374 + RR42_RS22965 0.90 +0.0 486,619 + RR42_RS22970 0.27 +0.9 486,766 + RR42_RS22970 0.48 -1.3 486,766 + RR42_RS22970 0.48 -0.1 486,767 - RR42_RS22970 0.48 +1.3 486,767 - RR42_RS22970 0.48 +0.1 486,807 + RR42_RS22970 0.54 +1.2 486,807 + RR42_RS22970 0.54 +0.6 486,807 + RR42_RS22970 0.54 +1.1 486,808 - RR42_RS22970 0.54 -0.6 486,808 - RR42_RS22970 0.54 +1.0 487,220 + -1.3 487,220 + +1.0 487,227 + -0.6 487,245 - +0.7 487,245 - -0.1 487,293 - -0.2 487,357 - +0.1 487,357 - +0.2 487,357 - -1.3 487,565 + RR42_RS22975 0.32 +1.2 487,565 + RR42_RS22975 0.32 -0.7 487,566 - RR42_RS22975 0.32 +0.8 487,566 - RR42_RS22975 0.32 -0.3 487,593 + RR42_RS22975 0.35 +0.1 487,593 + RR42_RS22975 0.35 -0.5 487,594 - RR42_RS22975 0.35 +0.3 487,859 - RR42_RS22975 0.64 +0.3 487,892 + RR42_RS22975 0.68 -0.1 487,892 + RR42_RS22975 0.68 +1.3 487,892 + RR42_RS22975 0.68 -0.1 487,893 - RR42_RS22975 0.68 +0.1 487,893 - RR42_RS22975 0.68 -1.0 488,050 - RR42_RS22975 0.85 -0.2 488,050 - RR42_RS22975 0.85 +0.5 488,050 - RR42_RS22975 0.85 +0.2 488,050 - RR42_RS22975 0.85 -0.4
Or see this region's nucleotide sequence