Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21965 and RR42_RS21970 are separated by 923 nucleotides RR42_RS21970 and RR42_RS21975 overlap by 38 nucleotides
RR42_RS21965: RR42_RS21965 - hypothetical protein, at 253,337 to 254,548
_RS21965
RR42_RS21970: RR42_RS21970 - hypothetical protein, at 255,472 to 258,339
_RS21970
RR42_RS21975: RR42_RS21975 - hypothetical protein, at 258,302 to 262,522
_RS21975
Position (kb)
255
256
257
258
259 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 254.616 kb on + strand at 254.617 kb on - strand at 254.623 kb on + strand at 254.623 kb on + strand at 254.624 kb on - strand at 254.630 kb on + strand at 254.631 kb on - strand at 254.754 kb on + strand at 254.831 kb on + strand at 254.949 kb on + strand at 254.950 kb on - strand at 254.950 kb on - strand at 254.950 kb on - strand at 254.950 kb on - strand at 254.950 kb on - strand at 254.957 kb on + strand at 254.957 kb on + strand at 254.957 kb on + strand at 254.958 kb on - strand at 254.958 kb on - strand at 255.135 kb on + strand at 255.135 kb on + strand at 255.135 kb on + strand at 255.135 kb on + strand at 255.135 kb on + strand at 255.136 kb on - strand at 255.136 kb on - strand at 255.149 kb on + strand at 255.156 kb on - strand at 255.156 kb on - strand at 255.163 kb on + strand at 255.163 kb on + strand at 255.164 kb on - strand at 255.164 kb on - strand at 255.164 kb on - strand at 255.165 kb on + strand at 255.165 kb on + strand at 255.210 kb on + strand at 255.210 kb on + strand at 255.211 kb on - strand at 255.684 kb on + strand at 255.700 kb on + strand at 255.701 kb on - strand at 256.055 kb on - strand, within RR42_RS21970 at 256.174 kb on + strand, within RR42_RS21970 at 256.273 kb on + strand, within RR42_RS21970 at 256.274 kb on - strand, within RR42_RS21970 at 256.417 kb on + strand, within RR42_RS21970 at 256.417 kb on + strand, within RR42_RS21970 at 256.418 kb on - strand, within RR42_RS21970 at 256.418 kb on - strand, within RR42_RS21970 at 256.418 kb on - strand, within RR42_RS21970 at 256.427 kb on - strand, within RR42_RS21970 at 256.811 kb on - strand, within RR42_RS21970 at 256.847 kb on + strand, within RR42_RS21970 at 256.848 kb on - strand, within RR42_RS21970 at 256.848 kb on - strand, within RR42_RS21970 at 256.858 kb on + strand, within RR42_RS21970 at 257.143 kb on + strand, within RR42_RS21970 at 257.143 kb on + strand, within RR42_RS21970 at 257.143 kb on + strand, within RR42_RS21970 at 257.143 kb on + strand, within RR42_RS21970 at 257.144 kb on - strand, within RR42_RS21970 at 257.200 kb on + strand, within RR42_RS21970 at 257.201 kb on - strand, within RR42_RS21970 at 257.323 kb on + strand, within RR42_RS21970 at 257.367 kb on + strand, within RR42_RS21970 at 257.367 kb on + strand, within RR42_RS21970 at 257.368 kb on - strand, within RR42_RS21970 at 257.368 kb on - strand, within RR42_RS21970 at 257.497 kb on - strand, within RR42_RS21970 at 257.577 kb on - strand, within RR42_RS21970 at 257.577 kb on - strand, within RR42_RS21970 at 257.605 kb on + strand, within RR42_RS21970 at 257.605 kb on + strand, within RR42_RS21970 at 257.605 kb on + strand, within RR42_RS21970 at 257.605 kb on + strand, within RR42_RS21970 at 257.629 kb on + strand, within RR42_RS21970 at 257.633 kb on - strand, within RR42_RS21970 at 257.633 kb on - strand, within RR42_RS21970 at 257.704 kb on + strand, within RR42_RS21970 at 257.704 kb on + strand, within RR42_RS21970 at 257.704 kb on + strand, within RR42_RS21970 at 257.704 kb on + strand, within RR42_RS21970 at 257.705 kb on - strand, within RR42_RS21970 at 257.705 kb on - strand, within RR42_RS21970 at 257.707 kb on + strand, within RR42_RS21970 at 257.707 kb on + strand, within RR42_RS21970 at 257.717 kb on - strand, within RR42_RS21970 at 257.761 kb on + strand, within RR42_RS21970 at 257.767 kb on + strand, within RR42_RS21970 at 257.810 kb on - strand, within RR42_RS21970 at 257.810 kb on - strand, within RR42_RS21970 at 258.004 kb on + strand, within RR42_RS21970 at 258.005 kb on - strand, within RR42_RS21970 at 258.060 kb on + strand at 258.173 kb on - strand at 258.549 kb on - strand at 258.773 kb on + strand, within RR42_RS21975 at 258.773 kb on + strand, within RR42_RS21975 at 258.774 kb on - strand, within RR42_RS21975 at 258.774 kb on - strand, within RR42_RS21975 at 258.897 kb on - strand, within RR42_RS21975 at 258.897 kb on - strand, within RR42_RS21975 at 259.052 kb on + strand, within RR42_RS21975 at 259.052 kb on + strand, within RR42_RS21975 at 259.052 kb on + strand, within RR42_RS21975 at 259.211 kb on + strand, within RR42_RS21975 at 259.212 kb on - strand, within RR42_RS21975 at 259.212 kb on - strand, within RR42_RS21975
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 254,616 + +0.2 254,617 - +0.6 254,623 + -0.7 254,623 + +0.5 254,624 - -0.4 254,630 + +0.4 254,631 - -0.3 254,754 + -0.3 254,831 + -0.1 254,949 + -0.6 254,950 - -0.3 254,950 - -0.3 254,950 - +0.8 254,950 - -3.2 254,950 - -0.6 254,957 + +0.0 254,957 + +0.7 254,957 + +0.3 254,958 - -0.1 254,958 - +0.6 255,135 + -0.5 255,135 + +2.1 255,135 + +0.1 255,135 + +0.1 255,135 + +0.2 255,136 - -0.4 255,136 - +0.5 255,149 + +1.3 255,156 - +0.8 255,156 - -0.4 255,163 + -0.2 255,163 + -0.3 255,164 - -1.7 255,164 - +0.7 255,164 - -0.0 255,165 + -0.5 255,165 + -0.1 255,210 + -0.1 255,210 + -1.0 255,211 - +0.1 255,684 + -0.3 255,700 + +0.8 255,701 - -0.2 256,055 - RR42_RS21970 0.20 +0.4 256,174 + RR42_RS21970 0.24 -0.6 256,273 + RR42_RS21970 0.28 +1.1 256,274 - RR42_RS21970 0.28 +0.4 256,417 + RR42_RS21970 0.33 -0.0 256,417 + RR42_RS21970 0.33 -0.2 256,418 - RR42_RS21970 0.33 +1.1 256,418 - RR42_RS21970 0.33 +0.2 256,418 - RR42_RS21970 0.33 -0.9 256,427 - RR42_RS21970 0.33 +1.3 256,811 - RR42_RS21970 0.47 -0.2 256,847 + RR42_RS21970 0.48 -1.6 256,848 - RR42_RS21970 0.48 -0.7 256,848 - RR42_RS21970 0.48 -0.7 256,858 + RR42_RS21970 0.48 +0.2 257,143 + RR42_RS21970 0.58 -0.7 257,143 + RR42_RS21970 0.58 +0.4 257,143 + RR42_RS21970 0.58 +0.7 257,143 + RR42_RS21970 0.58 +0.1 257,144 - RR42_RS21970 0.58 -0.6 257,200 + RR42_RS21970 0.60 -0.6 257,201 - RR42_RS21970 0.60 +0.8 257,323 + RR42_RS21970 0.65 -1.9 257,367 + RR42_RS21970 0.66 +0.4 257,367 + RR42_RS21970 0.66 +0.4 257,368 - RR42_RS21970 0.66 -0.5 257,368 - RR42_RS21970 0.66 -0.3 257,497 - RR42_RS21970 0.71 -0.4 257,577 - RR42_RS21970 0.73 -0.9 257,577 - RR42_RS21970 0.73 +0.4 257,605 + RR42_RS21970 0.74 +0.7 257,605 + RR42_RS21970 0.74 +0.2 257,605 + RR42_RS21970 0.74 -0.3 257,605 + RR42_RS21970 0.74 -0.3 257,629 + RR42_RS21970 0.75 +0.1 257,633 - RR42_RS21970 0.75 -0.5 257,633 - RR42_RS21970 0.75 -0.5 257,704 + RR42_RS21970 0.78 +0.3 257,704 + RR42_RS21970 0.78 +0.1 257,704 + RR42_RS21970 0.78 +0.5 257,704 + RR42_RS21970 0.78 -0.2 257,705 - RR42_RS21970 0.78 -0.2 257,705 - RR42_RS21970 0.78 +1.1 257,707 + RR42_RS21970 0.78 -0.5 257,707 + RR42_RS21970 0.78 -1.2 257,717 - RR42_RS21970 0.78 -1.6 257,761 + RR42_RS21970 0.80 +0.6 257,767 + RR42_RS21970 0.80 -0.5 257,810 - RR42_RS21970 0.82 +0.2 257,810 - RR42_RS21970 0.82 -0.5 258,004 + RR42_RS21970 0.88 -0.0 258,005 - RR42_RS21970 0.88 -0.3 258,060 + +0.8 258,173 - +0.0 258,549 - +0.9 258,773 + RR42_RS21975 0.11 +3.7 258,773 + RR42_RS21975 0.11 -0.7 258,774 - RR42_RS21975 0.11 -0.6 258,774 - RR42_RS21975 0.11 -0.2 258,897 - RR42_RS21975 0.14 +0.1 258,897 - RR42_RS21975 0.14 -0.7 259,052 + RR42_RS21975 0.18 -1.6 259,052 + RR42_RS21975 0.18 +1.2 259,052 + RR42_RS21975 0.18 -0.1 259,211 + RR42_RS21975 0.22 +0.7 259,212 - RR42_RS21975 0.22 +0.0 259,212 - RR42_RS21975 0.22 +1.0
Or see this region's nucleotide sequence