Experiment: nitrite 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20750 and SM_b20751 are separated by 30 nucleotides SM_b20751 and SM_b20752 are separated by 46 nucleotides SM_b20752 and SM_b20753 are separated by 12 nucleotides
SM_b20750: SM_b20750 - dehydrogenase, at 1,539,559 to 1,540,308
_b20750
SM_b20751: SM_b20751 - 3-hydroxyisobutyrate dehydrogenase, at 1,540,339 to 1,541,235
_b20751
SM_b20752: SM_b20752 - 3-hydroxyisobutyryl-CoA hydrolase, at 1,541,282 to 1,542,352
_b20752
SM_b20753: SM_b20753 - acyl-CoA dehydrogenase, at 1,542,365 to 1,543,507
_b20753
Position (kb)
1541
1542
1543 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1540.430 kb on + strand, within SM_b20751 at 1540.430 kb on + strand, within SM_b20751 at 1540.589 kb on + strand, within SM_b20751 at 1540.589 kb on + strand, within SM_b20751 at 1540.589 kb on + strand, within SM_b20751 at 1540.590 kb on - strand, within SM_b20751 at 1540.623 kb on + strand, within SM_b20751 at 1540.623 kb on + strand, within SM_b20751 at 1540.852 kb on - strand, within SM_b20751 at 1541.018 kb on + strand, within SM_b20751 at 1541.284 kb on - strand at 1541.284 kb on - strand at 1541.347 kb on + strand at 1541.856 kb on + strand, within SM_b20752 at 1541.856 kb on + strand, within SM_b20752 at 1541.856 kb on + strand, within SM_b20752 at 1541.857 kb on - strand, within SM_b20752 at 1541.860 kb on + strand, within SM_b20752 at 1542.032 kb on - strand, within SM_b20752 at 1542.033 kb on + strand, within SM_b20752 at 1542.033 kb on + strand, within SM_b20752 at 1542.034 kb on - strand, within SM_b20752 at 1542.046 kb on - strand, within SM_b20752 at 1542.127 kb on - strand, within SM_b20752 at 1542.127 kb on - strand, within SM_b20752 at 1542.128 kb on + strand, within SM_b20752 at 1542.129 kb on - strand, within SM_b20752 at 1542.262 kb on + strand at 1542.366 kb on + strand at 1542.366 kb on + strand at 1542.367 kb on - strand at 1542.367 kb on - strand at 1542.418 kb on + strand at 1542.429 kb on - strand at 1542.471 kb on + strand at 1542.490 kb on - strand, within SM_b20753 at 1542.723 kb on + strand, within SM_b20753 at 1542.723 kb on + strand, within SM_b20753 at 1542.723 kb on + strand, within SM_b20753 at 1542.724 kb on - strand, within SM_b20753 at 1542.724 kb on - strand, within SM_b20753 at 1542.724 kb on - strand, within SM_b20753 at 1542.724 kb on - strand, within SM_b20753 at 1542.778 kb on + strand, within SM_b20753 at 1543.012 kb on + strand, within SM_b20753 at 1543.013 kb on - strand, within SM_b20753 at 1543.013 kb on - strand, within SM_b20753 at 1543.014 kb on + strand, within SM_b20753 at 1543.014 kb on + strand, within SM_b20753 at 1543.014 kb on + strand, within SM_b20753 at 1543.015 kb on - strand, within SM_b20753 at 1543.069 kb on - strand, within SM_b20753 at 1543.143 kb on + strand, within SM_b20753 at 1543.143 kb on + strand, within SM_b20753 at 1543.144 kb on - strand, within SM_b20753 at 1543.144 kb on - strand, within SM_b20753 at 1543.315 kb on + strand, within SM_b20753 at 1543.316 kb on - strand, within SM_b20753 at 1543.347 kb on + strand, within SM_b20753 at 1543.347 kb on + strand, within SM_b20753 at 1543.347 kb on + strand, within SM_b20753 at 1543.347 kb on + strand, within SM_b20753 at 1543.347 kb on + strand, within SM_b20753 at 1543.347 kb on + strand, within SM_b20753 at 1543.347 kb on + strand, within SM_b20753 at 1543.348 kb on - strand, within SM_b20753
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 20 mM remove 1,540,430 + SM_b20751 0.10 +0.0 1,540,430 + SM_b20751 0.10 +0.2 1,540,589 + SM_b20751 0.28 -1.2 1,540,589 + SM_b20751 0.28 +0.1 1,540,589 + SM_b20751 0.28 +0.6 1,540,590 - SM_b20751 0.28 -0.5 1,540,623 + SM_b20751 0.32 -0.0 1,540,623 + SM_b20751 0.32 +0.2 1,540,852 - SM_b20751 0.57 -0.1 1,541,018 + SM_b20751 0.76 -0.3 1,541,284 - -0.4 1,541,284 - -0.3 1,541,347 + -0.2 1,541,856 + SM_b20752 0.54 -1.5 1,541,856 + SM_b20752 0.54 -0.6 1,541,856 + SM_b20752 0.54 -0.9 1,541,857 - SM_b20752 0.54 -1.9 1,541,860 + SM_b20752 0.54 -1.4 1,542,032 - SM_b20752 0.70 -1.5 1,542,033 + SM_b20752 0.70 +0.3 1,542,033 + SM_b20752 0.70 -1.8 1,542,034 - SM_b20752 0.70 -0.9 1,542,046 - SM_b20752 0.71 -1.1 1,542,127 - SM_b20752 0.79 -0.9 1,542,127 - SM_b20752 0.79 -1.2 1,542,128 + SM_b20752 0.79 -1.0 1,542,129 - SM_b20752 0.79 -0.8 1,542,262 + -0.1 1,542,366 + +1.5 1,542,366 + +1.8 1,542,367 - -1.1 1,542,367 - +1.2 1,542,418 + +0.2 1,542,429 - +0.1 1,542,471 + +0.6 1,542,490 - SM_b20753 0.11 +1.3 1,542,723 + SM_b20753 0.31 +0.2 1,542,723 + SM_b20753 0.31 -0.2 1,542,723 + SM_b20753 0.31 +0.5 1,542,724 - SM_b20753 0.31 +0.1 1,542,724 - SM_b20753 0.31 -0.4 1,542,724 - SM_b20753 0.31 -0.4 1,542,724 - SM_b20753 0.31 +0.6 1,542,778 + SM_b20753 0.36 +0.7 1,543,012 + SM_b20753 0.57 -1.1 1,543,013 - SM_b20753 0.57 +1.0 1,543,013 - SM_b20753 0.57 -0.1 1,543,014 + SM_b20753 0.57 +0.2 1,543,014 + SM_b20753 0.57 +0.1 1,543,014 + SM_b20753 0.57 -1.5 1,543,015 - SM_b20753 0.57 +0.9 1,543,069 - SM_b20753 0.62 +2.0 1,543,143 + SM_b20753 0.68 +0.1 1,543,143 + SM_b20753 0.68 +0.1 1,543,144 - SM_b20753 0.68 -0.2 1,543,144 - SM_b20753 0.68 +0.1 1,543,315 + SM_b20753 0.83 +1.5 1,543,316 - SM_b20753 0.83 -0.9 1,543,347 + SM_b20753 0.86 +0.1 1,543,347 + SM_b20753 0.86 -0.3 1,543,347 + SM_b20753 0.86 +0.3 1,543,347 + SM_b20753 0.86 +0.1 1,543,347 + SM_b20753 0.86 +0.9 1,543,347 + SM_b20753 0.86 +0.6 1,543,347 + SM_b20753 0.86 -0.2 1,543,348 - SM_b20753 0.86 +0.7
Or see this region's nucleotide sequence