Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30505 and RR42_RS30510 are separated by 61 nucleotides RR42_RS30510 and RR42_RS30515 are separated by 41 nucleotides RR42_RS30515 and RR42_RS30520 are separated by 66 nucleotides
RR42_RS30505: RR42_RS30505 - salicylate hydroxylase, at 2,218,035 to 2,219,243
_RS30505
RR42_RS30510: RR42_RS30510 - maleylacetoacetate isomerase, at 2,219,305 to 2,219,949
_RS30510
RR42_RS30515: RR42_RS30515 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 2,219,991 to 2,220,689
_RS30515
RR42_RS30520: RR42_RS30520 - gentisate 1,2-dioxygenase, at 2,220,756 to 2,221,802
_RS30520
Position (kb)
2219
2220
2221 Strain fitness (log2 ratio)
-2
-1
0
1 at 2219.012 kb on - strand, within RR42_RS30505 at 2219.319 kb on - strand at 2219.559 kb on - strand, within RR42_RS30510 at 2219.606 kb on + strand, within RR42_RS30510 at 2219.606 kb on + strand, within RR42_RS30510 at 2219.735 kb on + strand, within RR42_RS30510 at 2219.822 kb on + strand, within RR42_RS30510 at 2219.822 kb on + strand, within RR42_RS30510 at 2219.822 kb on + strand, within RR42_RS30510 at 2219.822 kb on + strand, within RR42_RS30510 at 2219.823 kb on - strand, within RR42_RS30510 at 2219.937 kb on + strand at 2219.938 kb on - strand at 2219.938 kb on - strand at 2219.939 kb on + strand at 2220.089 kb on - strand, within RR42_RS30515 at 2220.119 kb on - strand, within RR42_RS30515 at 2220.401 kb on + strand, within RR42_RS30515 at 2220.402 kb on - strand, within RR42_RS30515 at 2220.445 kb on + strand, within RR42_RS30515 at 2220.446 kb on - strand, within RR42_RS30515 at 2220.451 kb on + strand, within RR42_RS30515 at 2220.451 kb on + strand, within RR42_RS30515 at 2220.451 kb on + strand, within RR42_RS30515 at 2220.452 kb on - strand, within RR42_RS30515 at 2220.452 kb on - strand, within RR42_RS30515 at 2220.452 kb on - strand, within RR42_RS30515 at 2220.452 kb on - strand, within RR42_RS30515 at 2220.589 kb on + strand, within RR42_RS30515 at 2220.590 kb on - strand, within RR42_RS30515 at 2220.674 kb on + strand at 2220.738 kb on + strand at 2220.738 kb on + strand at 2220.738 kb on + strand at 2220.738 kb on + strand at 2220.738 kb on + strand at 2220.739 kb on - strand at 2220.739 kb on - strand at 2220.793 kb on - strand at 2220.811 kb on + strand at 2220.946 kb on + strand, within RR42_RS30520 at 2220.946 kb on + strand, within RR42_RS30520 at 2220.946 kb on + strand, within RR42_RS30520 at 2220.947 kb on - strand, within RR42_RS30520 at 2220.947 kb on - strand, within RR42_RS30520 at 2220.947 kb on - strand, within RR42_RS30520 at 2220.947 kb on - strand, within RR42_RS30520 at 2220.971 kb on - strand, within RR42_RS30520 at 2221.000 kb on + strand, within RR42_RS30520 at 2221.013 kb on + strand, within RR42_RS30520 at 2221.014 kb on - strand, within RR42_RS30520 at 2221.057 kb on + strand, within RR42_RS30520 at 2221.058 kb on - strand, within RR42_RS30520 at 2221.136 kb on - strand, within RR42_RS30520 at 2221.141 kb on + strand, within RR42_RS30520 at 2221.141 kb on + strand, within RR42_RS30520 at 2221.142 kb on - strand, within RR42_RS30520 at 2221.258 kb on + strand, within RR42_RS30520 at 2221.461 kb on - strand, within RR42_RS30520 at 2221.461 kb on - strand, within RR42_RS30520 at 2221.575 kb on - strand, within RR42_RS30520 at 2221.660 kb on + strand, within RR42_RS30520
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 2,219,012 - RR42_RS30505 0.81 +0.0 2,219,319 - +0.4 2,219,559 - RR42_RS30510 0.39 +0.2 2,219,606 + RR42_RS30510 0.47 -0.9 2,219,606 + RR42_RS30510 0.47 -0.6 2,219,735 + RR42_RS30510 0.67 +0.4 2,219,822 + RR42_RS30510 0.80 -0.6 2,219,822 + RR42_RS30510 0.80 -0.6 2,219,822 + RR42_RS30510 0.80 +1.6 2,219,822 + RR42_RS30510 0.80 +0.8 2,219,823 - RR42_RS30510 0.80 +1.0 2,219,937 + +0.2 2,219,938 - -0.1 2,219,938 - -1.4 2,219,939 + -1.3 2,220,089 - RR42_RS30515 0.14 +0.7 2,220,119 - RR42_RS30515 0.18 +0.1 2,220,401 + RR42_RS30515 0.59 -0.3 2,220,402 - RR42_RS30515 0.59 +0.1 2,220,445 + RR42_RS30515 0.65 +0.2 2,220,446 - RR42_RS30515 0.65 -0.2 2,220,451 + RR42_RS30515 0.66 +0.4 2,220,451 + RR42_RS30515 0.66 -1.2 2,220,451 + RR42_RS30515 0.66 +1.2 2,220,452 - RR42_RS30515 0.66 +1.0 2,220,452 - RR42_RS30515 0.66 -0.6 2,220,452 - RR42_RS30515 0.66 -0.0 2,220,452 - RR42_RS30515 0.66 +0.4 2,220,589 + RR42_RS30515 0.86 -0.4 2,220,590 - RR42_RS30515 0.86 -0.6 2,220,674 + -0.3 2,220,738 + -0.8 2,220,738 + -0.1 2,220,738 + -1.4 2,220,738 + -0.1 2,220,738 + -0.0 2,220,739 - -0.3 2,220,739 - -0.0 2,220,793 - -0.4 2,220,811 + +0.7 2,220,946 + RR42_RS30520 0.18 -0.1 2,220,946 + RR42_RS30520 0.18 +0.5 2,220,946 + RR42_RS30520 0.18 -0.4 2,220,947 - RR42_RS30520 0.18 -0.6 2,220,947 - RR42_RS30520 0.18 +0.1 2,220,947 - RR42_RS30520 0.18 +0.2 2,220,947 - RR42_RS30520 0.18 +1.7 2,220,971 - RR42_RS30520 0.21 +0.3 2,221,000 + RR42_RS30520 0.23 +0.4 2,221,013 + RR42_RS30520 0.25 -0.9 2,221,014 - RR42_RS30520 0.25 -1.7 2,221,057 + RR42_RS30520 0.29 +0.8 2,221,058 - RR42_RS30520 0.29 -2.1 2,221,136 - RR42_RS30520 0.36 -0.4 2,221,141 + RR42_RS30520 0.37 -0.3 2,221,141 + RR42_RS30520 0.37 +1.6 2,221,142 - RR42_RS30520 0.37 -0.0 2,221,258 + RR42_RS30520 0.48 +1.6 2,221,461 - RR42_RS30520 0.67 -0.1 2,221,461 - RR42_RS30520 0.67 +0.2 2,221,575 - RR42_RS30520 0.78 +0.2 2,221,660 + RR42_RS30520 0.86 +0.8
Or see this region's nucleotide sequence