Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25515 and RR42_RS25520 are separated by 50 nucleotides RR42_RS25520 and RR42_RS25525 overlap by 4 nucleotides
RR42_RS25515: RR42_RS25515 - AMP-dependent synthetase, at 1,069,867 to 1,071,327
_RS25515
RR42_RS25520: RR42_RS25520 - alpha/beta hydrolase, at 1,071,378 to 1,072,310
_RS25520
RR42_RS25525: RR42_RS25525 - enoyl-CoA hydratase, at 1,072,307 to 1,073,281
_RS25525
Position (kb)
1071
1072
1073 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1070.501 kb on + strand, within RR42_RS25515 at 1070.501 kb on + strand, within RR42_RS25515 at 1070.501 kb on + strand, within RR42_RS25515 at 1070.502 kb on - strand, within RR42_RS25515 at 1070.618 kb on + strand, within RR42_RS25515 at 1070.619 kb on - strand, within RR42_RS25515 at 1070.642 kb on + strand, within RR42_RS25515 at 1070.643 kb on - strand, within RR42_RS25515 at 1070.643 kb on - strand, within RR42_RS25515 at 1070.897 kb on + strand, within RR42_RS25515 at 1070.898 kb on - strand, within RR42_RS25515 at 1071.018 kb on + strand, within RR42_RS25515 at 1071.340 kb on + strand at 1071.340 kb on + strand at 1071.341 kb on - strand at 1071.344 kb on + strand at 1071.454 kb on + strand at 1071.455 kb on - strand at 1071.685 kb on + strand, within RR42_RS25520 at 1071.807 kb on - strand, within RR42_RS25520 at 1071.840 kb on - strand, within RR42_RS25520 at 1071.942 kb on - strand, within RR42_RS25520 at 1072.017 kb on + strand, within RR42_RS25520 at 1072.018 kb on - strand, within RR42_RS25520 at 1072.079 kb on - strand, within RR42_RS25520 at 1072.079 kb on - strand, within RR42_RS25520 at 1072.229 kb on - strand at 1072.365 kb on + strand at 1072.522 kb on - strand, within RR42_RS25525 at 1072.573 kb on - strand, within RR42_RS25525 at 1072.760 kb on + strand, within RR42_RS25525 at 1072.760 kb on + strand, within RR42_RS25525 at 1072.761 kb on - strand, within RR42_RS25525 at 1072.807 kb on + strand, within RR42_RS25525 at 1073.028 kb on - strand, within RR42_RS25525 at 1073.111 kb on + strand, within RR42_RS25525 at 1073.112 kb on - strand, within RR42_RS25525 at 1073.243 kb on + strand at 1073.243 kb on + strand at 1073.243 kb on + strand at 1073.244 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 1,070,501 + RR42_RS25515 0.43 +0.2 1,070,501 + RR42_RS25515 0.43 -0.6 1,070,501 + RR42_RS25515 0.43 -0.1 1,070,502 - RR42_RS25515 0.43 -1.8 1,070,618 + RR42_RS25515 0.51 +0.1 1,070,619 - RR42_RS25515 0.51 +0.3 1,070,642 + RR42_RS25515 0.53 -0.9 1,070,643 - RR42_RS25515 0.53 -0.0 1,070,643 - RR42_RS25515 0.53 +1.8 1,070,897 + RR42_RS25515 0.70 -0.1 1,070,898 - RR42_RS25515 0.71 +0.4 1,071,018 + RR42_RS25515 0.79 -1.6 1,071,340 + -0.5 1,071,340 + +0.9 1,071,341 - +1.4 1,071,344 + +0.6 1,071,454 + -0.3 1,071,455 - +0.4 1,071,685 + RR42_RS25520 0.33 +0.3 1,071,807 - RR42_RS25520 0.46 -0.4 1,071,840 - RR42_RS25520 0.50 -0.3 1,071,942 - RR42_RS25520 0.60 -0.9 1,072,017 + RR42_RS25520 0.68 +0.5 1,072,018 - RR42_RS25520 0.69 -1.6 1,072,079 - RR42_RS25520 0.75 -0.5 1,072,079 - RR42_RS25520 0.75 -0.4 1,072,229 - +0.9 1,072,365 + +1.3 1,072,522 - RR42_RS25525 0.22 +0.2 1,072,573 - RR42_RS25525 0.27 +0.5 1,072,760 + RR42_RS25525 0.46 -0.0 1,072,760 + RR42_RS25525 0.46 +0.5 1,072,761 - RR42_RS25525 0.47 -1.3 1,072,807 + RR42_RS25525 0.51 -0.5 1,073,028 - RR42_RS25525 0.74 +0.6 1,073,111 + RR42_RS25525 0.82 +0.3 1,073,112 - RR42_RS25525 0.83 +0.4 1,073,243 + -1.5 1,073,243 + -1.1 1,073,243 + +0.0 1,073,244 - +0.2
Or see this region's nucleotide sequence