Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22035 and RR42_RS22040 are separated by 100 nucleotides RR42_RS22040 and RR42_RS22045 are separated by 15 nucleotides
RR42_RS22035: RR42_RS22035 - ATPase, at 275,686 to 278,376
_RS22035
RR42_RS22040: RR42_RS22040 - Hcp family T6SS protein CtsH1, at 278,477 to 278,962
_RS22040
RR42_RS22045: RR42_RS22045 - flagellar motor protein MotB, at 278,978 to 280,693
_RS22045
Position (kb) 278
279 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 277.555 kb on + strand, within RR42_RS22035 at 277.555 kb on + strand, within RR42_RS22035 at 277.556 kb on - strand, within RR42_RS22035 at 277.556 kb on - strand, within RR42_RS22035 at 277.556 kb on - strand, within RR42_RS22035 at 278.198 kb on + strand at 278.422 kb on + strand at 278.527 kb on - strand, within RR42_RS22040 at 278.695 kb on - strand, within RR42_RS22040 at 278.746 kb on - strand, within RR42_RS22040 at 278.746 kb on - strand, within RR42_RS22040 at 278.752 kb on - strand, within RR42_RS22040 at 278.752 kb on - strand, within RR42_RS22040 at 278.946 kb on + strand at 278.946 kb on + strand at 278.946 kb on + strand at 278.946 kb on + strand at 278.947 kb on - strand at 278.947 kb on - strand at 278.947 kb on - strand at 278.947 kb on - strand at 278.947 kb on - strand at 279.127 kb on - strand at 279.127 kb on - strand at 279.456 kb on + strand, within RR42_RS22045 at 279.592 kb on + strand, within RR42_RS22045 at 279.825 kb on + strand, within RR42_RS22045 at 279.825 kb on + strand, within RR42_RS22045
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 277,555 + RR42_RS22035 0.69 +0.3 277,555 + RR42_RS22035 0.69 -0.9 277,556 - RR42_RS22035 0.69 +1.3 277,556 - RR42_RS22035 0.69 +0.2 277,556 - RR42_RS22035 0.69 -0.3 278,198 + +0.7 278,422 + -0.8 278,527 - RR42_RS22040 0.10 -0.1 278,695 - RR42_RS22040 0.45 +0.1 278,746 - RR42_RS22040 0.55 +0.6 278,746 - RR42_RS22040 0.55 -1.5 278,752 - RR42_RS22040 0.57 +0.4 278,752 - RR42_RS22040 0.57 +2.1 278,946 + -0.0 278,946 + -2.2 278,946 + -0.5 278,946 + +1.6 278,947 - +0.5 278,947 - +0.8 278,947 - +0.1 278,947 - -0.2 278,947 - +0.6 279,127 - +0.3 279,127 - -0.6 279,456 + RR42_RS22045 0.28 -3.1 279,592 + RR42_RS22045 0.36 +0.4 279,825 + RR42_RS22045 0.49 -1.8 279,825 + RR42_RS22045 0.49 +2.0
Or see this region's nucleotide sequence