Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07405 and MMP_RS07410 are separated by 1 nucleotides MMP_RS07410 and MMP_RS07415 are separated by 107 nucleotides MMP_RS07415 and MMP_RS07420 are separated by 110 nucleotides
MMP_RS07405: MMP_RS07405 - 2 3-bisphosphoglycerate-independent phosphoglycerate mutase, at 1,410,479 to 1,411,699
_RS07405
MMP_RS07410: MMP_RS07410 - 4-demethylwyosine synthase TYW1, at 1,411,701 to 1,412,642
_RS07410
MMP_RS07415: MMP_RS07415 - molybdopterin-guanine dinucleotide biosynthesis protein MobB, at 1,412,750 to 1,413,439
_RS07415
MMP_RS07420: MMP_RS07420 - metalloregulator ArsR/SmtB family transcription factor, at 1,413,550 to 1,413,837
_RS07420
Position (kb)
1411
1412
1413 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1410.788 kb on + strand at 1410.793 kb on + strand at 1411.131 kb on - strand at 1411.131 kb on - strand at 1411.131 kb on - strand at 1411.646 kb on + strand at 1411.646 kb on + strand at 1411.698 kb on + strand at 1411.698 kb on + strand at 1411.702 kb on - strand at 1411.716 kb on - strand at 1411.743 kb on + strand at 1411.748 kb on - strand at 1411.749 kb on + strand at 1411.846 kb on + strand, within MMP_RS07410 at 1412.140 kb on + strand, within MMP_RS07410 at 1412.167 kb on + strand, within MMP_RS07410 at 1412.205 kb on - strand, within MMP_RS07410 at 1412.205 kb on - strand, within MMP_RS07410 at 1412.206 kb on + strand, within MMP_RS07410 at 1412.216 kb on - strand, within MMP_RS07410 at 1412.216 kb on - strand, within MMP_RS07410 at 1412.216 kb on - strand, within MMP_RS07410 at 1412.217 kb on + strand, within MMP_RS07410 at 1412.218 kb on - strand, within MMP_RS07410 at 1412.219 kb on + strand, within MMP_RS07410 at 1412.224 kb on - strand, within MMP_RS07410 at 1412.224 kb on - strand, within MMP_RS07410 at 1412.225 kb on + strand, within MMP_RS07410 at 1412.231 kb on - strand, within MMP_RS07410 at 1412.231 kb on - strand, within MMP_RS07410 at 1412.320 kb on - strand, within MMP_RS07410 at 1412.329 kb on + strand, within MMP_RS07410 at 1412.331 kb on + strand, within MMP_RS07410 at 1412.352 kb on - strand, within MMP_RS07410 at 1412.354 kb on - strand, within MMP_RS07410 at 1412.355 kb on + strand, within MMP_RS07410 at 1412.445 kb on + strand, within MMP_RS07410 at 1412.522 kb on - strand, within MMP_RS07410 at 1412.619 kb on + strand at 1412.654 kb on - strand at 1412.654 kb on - strand at 1412.657 kb on + strand at 1412.672 kb on + strand at 1412.675 kb on - strand at 1412.679 kb on + strand at 1412.682 kb on - strand at 1412.691 kb on - strand at 1412.696 kb on - strand at 1412.696 kb on - strand at 1412.705 kb on - strand at 1412.705 kb on - strand at 1412.741 kb on + strand at 1412.741 kb on + strand at 1412.745 kb on + strand at 1412.745 kb on + strand at 1412.750 kb on + strand at 1412.763 kb on - strand at 1412.763 kb on - strand at 1412.763 kb on - strand at 1413.055 kb on - strand, within MMP_RS07415 at 1413.067 kb on + strand, within MMP_RS07415 at 1413.073 kb on + strand, within MMP_RS07415 at 1413.195 kb on + strand, within MMP_RS07415 at 1413.297 kb on - strand, within MMP_RS07415 at 1413.365 kb on + strand, within MMP_RS07415 at 1413.365 kb on + strand, within MMP_RS07415 at 1413.366 kb on - strand, within MMP_RS07415 at 1413.378 kb on - strand at 1413.379 kb on + strand at 1413.379 kb on + strand at 1413.445 kb on + strand at 1413.475 kb on - strand at 1413.526 kb on + strand at 1413.527 kb on - strand at 1413.557 kb on - strand at 1413.571 kb on - strand at 1413.615 kb on - strand, within MMP_RS07420 at 1413.616 kb on + strand, within MMP_RS07420 at 1413.639 kb on + strand, within MMP_RS07420
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE remove 1,410,788 + -3.3 1,410,793 + -3.3 1,411,131 - -1.8 1,411,131 - -3.8 1,411,131 - -3.1 1,411,646 + -2.9 1,411,646 + -2.7 1,411,698 + +0.1 1,411,698 + +0.7 1,411,702 - +0.3 1,411,716 - +0.1 1,411,743 + -0.2 1,411,748 - -0.9 1,411,749 + -1.1 1,411,846 + MMP_RS07410 0.15 -2.5 1,412,140 + MMP_RS07410 0.47 -2.5 1,412,167 + MMP_RS07410 0.49 -1.8 1,412,205 - MMP_RS07410 0.54 -1.7 1,412,205 - MMP_RS07410 0.54 -0.8 1,412,206 + MMP_RS07410 0.54 -1.2 1,412,216 - MMP_RS07410 0.55 -2.3 1,412,216 - MMP_RS07410 0.55 -2.5 1,412,216 - MMP_RS07410 0.55 -2.1 1,412,217 + MMP_RS07410 0.55 -1.8 1,412,218 - MMP_RS07410 0.55 -1.5 1,412,219 + MMP_RS07410 0.55 -2.8 1,412,224 - MMP_RS07410 0.56 -1.3 1,412,224 - MMP_RS07410 0.56 -1.6 1,412,225 + MMP_RS07410 0.56 -1.3 1,412,231 - MMP_RS07410 0.56 -1.3 1,412,231 - MMP_RS07410 0.56 -2.1 1,412,320 - MMP_RS07410 0.66 -1.5 1,412,329 + MMP_RS07410 0.67 -1.8 1,412,331 + MMP_RS07410 0.67 -1.9 1,412,352 - MMP_RS07410 0.69 -1.1 1,412,354 - MMP_RS07410 0.69 -2.2 1,412,355 + MMP_RS07410 0.69 -1.7 1,412,445 + MMP_RS07410 0.79 -0.9 1,412,522 - MMP_RS07410 0.87 -1.5 1,412,619 + -1.3 1,412,654 - -0.9 1,412,654 - -0.6 1,412,657 + -1.0 1,412,672 + -0.8 1,412,675 - -0.1 1,412,679 + -1.0 1,412,682 - -0.5 1,412,691 - -0.0 1,412,696 - -0.1 1,412,696 - +0.2 1,412,705 - +0.0 1,412,705 - +0.4 1,412,741 + -0.1 1,412,741 + +0.2 1,412,745 + +0.5 1,412,745 + +0.1 1,412,750 + +0.2 1,412,763 - +0.4 1,412,763 - +0.7 1,412,763 - -0.3 1,413,055 - MMP_RS07415 0.44 +0.4 1,413,067 + MMP_RS07415 0.46 -0.1 1,413,073 + MMP_RS07415 0.47 -0.9 1,413,195 + MMP_RS07415 0.64 +0.4 1,413,297 - MMP_RS07415 0.79 +0.2 1,413,365 + MMP_RS07415 0.89 -0.1 1,413,365 + MMP_RS07415 0.89 +0.3 1,413,366 - MMP_RS07415 0.89 -0.2 1,413,378 - -0.3 1,413,379 + -0.4 1,413,379 + -1.0 1,413,445 + +0.7 1,413,475 - +0.7 1,413,526 + -1.8 1,413,527 - -2.6 1,413,557 - -2.4 1,413,571 - -1.1 1,413,615 - MMP_RS07420 0.23 -2.3 1,413,616 + MMP_RS07420 0.23 -3.5 1,413,639 + MMP_RS07420 0.31 -1.9
Or see this region's nucleotide sequence