Strain Fitness in Methanococcus maripaludis S2 around MMP_RS05845

Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS05840 and MMP_RS05845 are separated by 186 nucleotidesMMP_RS05845 and MMP_RS05850 are separated by 17 nucleotides MMP_RS05840: MMP_RS05840 - peptide chain release factor aRF-1, at 1,120,835 to 1,122,094 _RS05840 MMP_RS05845: MMP_RS05845 - tRNA-intron lyase, at 1,122,281 to 1,122,814 _RS05845 MMP_RS05850: MMP_RS05850 - Ldh family oxidoreductase, at 1,122,832 to 1,123,875 _RS05850 Position (kb) 1122 1123Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1121.825 kb on - strandat 1122.094 kb on - strandat 1122.184 kb on - strandat 1122.184 kb on - strandat 1122.228 kb on + strandat 1122.229 kb on - strandat 1122.230 kb on + strandat 1122.233 kb on + strandat 1122.236 kb on - strandat 1122.236 kb on - strandat 1122.280 kb on - strandat 1122.280 kb on - strandat 1122.281 kb on + strandat 1122.466 kb on - strand, within MMP_RS05845at 1122.747 kb on - strand, within MMP_RS05845at 1122.760 kb on + strand, within MMP_RS05845at 1122.760 kb on + strand, within MMP_RS05845at 1123.047 kb on + strand, within MMP_RS05850at 1123.173 kb on - strand, within MMP_RS05850at 1123.437 kb on + strand, within MMP_RS05850at 1123.764 kb on - strand, within MMP_RS05850at 1123.765 kb on + strand, within MMP_RS05850at 1123.808 kb on - strandat 1123.809 kb on + strandat 1123.809 kb on + strandat 1123.809 kb on + strandat 1123.809 kb on + strandat 1123.809 kb on + strandat 1123.812 kb on + strandat 1123.812 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE
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1,121,825 - -3.7
1,122,094 - -3.8
1,122,184 - -3.4
1,122,184 - -3.4
1,122,228 + -0.3
1,122,229 - -2.3
1,122,230 + -1.2
1,122,233 + -0.3
1,122,236 - -1.5
1,122,236 - -2.1
1,122,280 - -2.1
1,122,280 - -1.8
1,122,281 + -4.0
1,122,466 - MMP_RS05845 0.35 -1.0
1,122,747 - MMP_RS05845 0.87 -3.4
1,122,760 + MMP_RS05845 0.90 -2.4
1,122,760 + MMP_RS05845 0.90 -3.3
1,123,047 + MMP_RS05850 0.21 -1.6
1,123,173 - MMP_RS05850 0.33 -2.1
1,123,437 + MMP_RS05850 0.58 -0.7
1,123,764 - MMP_RS05850 0.89 -1.7
1,123,765 + MMP_RS05850 0.89 -0.3
1,123,808 - -1.6
1,123,809 + -1.7
1,123,809 + -2.2
1,123,809 + -1.7
1,123,809 + -2.1
1,123,809 + -1.9
1,123,812 + -1.4
1,123,812 + -0.4

Or see this region's nucleotide sequence