Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05060 and MMP_RS05065 are separated by 4 nucleotides MMP_RS05065 and MMP_RS05070 are separated by 104 nucleotides MMP_RS05070 and MMP_RS05075 are separated by 4 nucleotides MMP_RS05075 and MMP_RS05080 are separated by 13 nucleotides
MMP_RS05060: MMP_RS05060 - AAA family ATPase, at 963,269 to 964,051
_RS05060
MMP_RS05065: MMP_RS05065 - CooT family nickel-binding protein, at 964,056 to 964,235
_RS05065
MMP_RS05070: MMP_RS05070 - 4Fe-4S dicluster domain-containing protein, at 964,340 to 964,804
_RS05070
MMP_RS05075: MMP_RS05075 - acetyl-CoA decarbonylase/synthase complex subunit gamma, at 964,809 to 966,182
_RS05075
MMP_RS05080: MMP_RS05080 - acetyl-CoA decarbonylase/synthase complex subunit delta, at 966,196 to 967,350
_RS05080
Position (kb) 964
965
966
967 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 963.847 kb on + strand, within MMP_RS05060 at 963.916 kb on + strand, within MMP_RS05060 at 963.919 kb on + strand, within MMP_RS05060 at 963.945 kb on - strand, within MMP_RS05060 at 963.946 kb on + strand, within MMP_RS05060 at 964.013 kb on + strand at 964.060 kb on + strand at 964.075 kb on + strand, within MMP_RS05065 at 964.075 kb on + strand, within MMP_RS05065 at 964.075 kb on + strand, within MMP_RS05065 at 964.138 kb on + strand, within MMP_RS05065 at 964.197 kb on - strand, within MMP_RS05065 at 964.235 kb on - strand at 964.267 kb on - strand at 964.288 kb on + strand at 964.301 kb on + strand at 964.304 kb on + strand at 964.354 kb on + strand at 964.402 kb on - strand, within MMP_RS05070 at 964.421 kb on - strand, within MMP_RS05070 at 964.533 kb on + strand, within MMP_RS05070 at 964.533 kb on + strand, within MMP_RS05070 at 964.537 kb on - strand, within MMP_RS05070 at 964.537 kb on - strand, within MMP_RS05070 at 964.581 kb on + strand, within MMP_RS05070 at 964.581 kb on + strand, within MMP_RS05070 at 964.582 kb on - strand, within MMP_RS05070 at 964.601 kb on - strand, within MMP_RS05070 at 964.751 kb on + strand, within MMP_RS05070 at 964.787 kb on - strand at 964.816 kb on + strand at 964.824 kb on - strand at 964.825 kb on + strand at 964.866 kb on + strand at 964.891 kb on + strand at 964.947 kb on - strand, within MMP_RS05075 at 965.209 kb on - strand, within MMP_RS05075 at 965.252 kb on - strand, within MMP_RS05075 at 965.256 kb on - strand, within MMP_RS05075 at 965.265 kb on - strand, within MMP_RS05075 at 965.439 kb on + strand, within MMP_RS05075 at 965.477 kb on + strand, within MMP_RS05075 at 965.496 kb on - strand, within MMP_RS05075 at 965.496 kb on - strand, within MMP_RS05075 at 965.497 kb on + strand, within MMP_RS05075 at 965.497 kb on + strand, within MMP_RS05075 at 965.598 kb on - strand, within MMP_RS05075 at 965.621 kb on - strand, within MMP_RS05075 at 965.639 kb on + strand, within MMP_RS05075 at 965.790 kb on - strand, within MMP_RS05075 at 965.791 kb on + strand, within MMP_RS05075 at 965.890 kb on + strand, within MMP_RS05075 at 965.890 kb on + strand, within MMP_RS05075 at 965.890 kb on + strand, within MMP_RS05075 at 965.917 kb on - strand, within MMP_RS05075 at 965.918 kb on + strand, within MMP_RS05075 at 965.918 kb on + strand, within MMP_RS05075 at 965.921 kb on + strand, within MMP_RS05075 at 965.936 kb on - strand, within MMP_RS05075 at 965.936 kb on - strand, within MMP_RS05075 at 965.937 kb on + strand, within MMP_RS05075 at 965.938 kb on - strand, within MMP_RS05075 at 965.939 kb on + strand, within MMP_RS05075 at 965.939 kb on + strand, within MMP_RS05075 at 965.940 kb on - strand, within MMP_RS05075 at 965.941 kb on + strand, within MMP_RS05075 at 965.941 kb on + strand, within MMP_RS05075 at 965.941 kb on + strand, within MMP_RS05075 at 966.017 kb on - strand, within MMP_RS05075 at 966.047 kb on - strand at 966.108 kb on + strand at 966.161 kb on - strand at 966.161 kb on - strand at 966.209 kb on + strand at 966.222 kb on - strand at 966.223 kb on + strand at 966.252 kb on + strand at 966.313 kb on - strand, within MMP_RS05080 at 966.313 kb on - strand, within MMP_RS05080 at 966.505 kb on - strand, within MMP_RS05080 at 966.559 kb on - strand, within MMP_RS05080 at 966.559 kb on - strand, within MMP_RS05080 at 966.560 kb on + strand, within MMP_RS05080 at 966.578 kb on + strand, within MMP_RS05080 at 966.591 kb on - strand, within MMP_RS05080 at 966.622 kb on - strand, within MMP_RS05080 at 966.623 kb on + strand, within MMP_RS05080 at 966.623 kb on + strand, within MMP_RS05080 at 966.864 kb on - strand, within MMP_RS05080 at 966.910 kb on + strand, within MMP_RS05080 at 966.964 kb on - strand, within MMP_RS05080 at 966.964 kb on - strand, within MMP_RS05080 at 966.965 kb on + strand, within MMP_RS05080 at 966.965 kb on + strand, within MMP_RS05080 at 967.021 kb on - strand, within MMP_RS05080 at 967.022 kb on + strand, within MMP_RS05080
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE remove 963,847 + MMP_RS05060 0.74 -3.7 963,916 + MMP_RS05060 0.83 -3.7 963,919 + MMP_RS05060 0.83 -3.8 963,945 - MMP_RS05060 0.86 -3.5 963,946 + MMP_RS05060 0.86 -4.9 964,013 + -3.1 964,060 + -3.1 964,075 + MMP_RS05065 0.11 -3.4 964,075 + MMP_RS05065 0.11 -3.7 964,075 + MMP_RS05065 0.11 -4.0 964,138 + MMP_RS05065 0.46 -2.7 964,197 - MMP_RS05065 0.78 -3.2 964,235 - -3.2 964,267 - -3.5 964,288 + -4.4 964,301 + -3.1 964,304 + -2.2 964,354 + -2.6 964,402 - MMP_RS05070 0.13 -2.5 964,421 - MMP_RS05070 0.17 -4.0 964,533 + MMP_RS05070 0.42 -4.1 964,533 + MMP_RS05070 0.42 -3.6 964,537 - MMP_RS05070 0.42 -3.9 964,537 - MMP_RS05070 0.42 -2.0 964,581 + MMP_RS05070 0.52 -5.6 964,581 + MMP_RS05070 0.52 -5.3 964,582 - MMP_RS05070 0.52 -3.1 964,601 - MMP_RS05070 0.56 -5.5 964,751 + MMP_RS05070 0.88 -3.9 964,787 - -4.9 964,816 + -3.5 964,824 - -5.2 964,825 + -3.2 964,866 + -4.4 964,891 + -3.6 964,947 - MMP_RS05075 0.10 -5.0 965,209 - MMP_RS05075 0.29 -3.0 965,252 - MMP_RS05075 0.32 -3.6 965,256 - MMP_RS05075 0.33 -4.4 965,265 - MMP_RS05075 0.33 -5.2 965,439 + MMP_RS05075 0.46 -6.0 965,477 + MMP_RS05075 0.49 -4.3 965,496 - MMP_RS05075 0.50 -4.0 965,496 - MMP_RS05075 0.50 -4.6 965,497 + MMP_RS05075 0.50 -5.3 965,497 + MMP_RS05075 0.50 -4.4 965,598 - MMP_RS05075 0.57 -5.7 965,621 - MMP_RS05075 0.59 -4.6 965,639 + MMP_RS05075 0.60 -4.4 965,790 - MMP_RS05075 0.71 -4.1 965,791 + MMP_RS05075 0.71 -3.4 965,890 + MMP_RS05075 0.79 -4.8 965,890 + MMP_RS05075 0.79 -4.5 965,890 + MMP_RS05075 0.79 -4.0 965,917 - MMP_RS05075 0.81 -3.0 965,918 + MMP_RS05075 0.81 -3.1 965,918 + MMP_RS05075 0.81 -3.2 965,921 + MMP_RS05075 0.81 -4.5 965,936 - MMP_RS05075 0.82 -4.2 965,936 - MMP_RS05075 0.82 -4.3 965,937 + MMP_RS05075 0.82 -5.0 965,938 - MMP_RS05075 0.82 -2.2 965,939 + MMP_RS05075 0.82 -4.1 965,939 + MMP_RS05075 0.82 -4.3 965,940 - MMP_RS05075 0.82 -6.4 965,941 + MMP_RS05075 0.82 -3.5 965,941 + MMP_RS05075 0.82 -3.2 965,941 + MMP_RS05075 0.82 -4.3 966,017 - MMP_RS05075 0.88 -3.8 966,047 - -4.1 966,108 + -4.2 966,161 - -5.2 966,161 - -5.3 966,209 + -5.7 966,222 - -3.6 966,223 + -5.4 966,252 + -3.2 966,313 - MMP_RS05080 0.10 -4.5 966,313 - MMP_RS05080 0.10 -5.4 966,505 - MMP_RS05080 0.27 -4.9 966,559 - MMP_RS05080 0.31 -4.4 966,559 - MMP_RS05080 0.31 -4.7 966,560 + MMP_RS05080 0.32 -3.9 966,578 + MMP_RS05080 0.33 -3.8 966,591 - MMP_RS05080 0.34 -5.2 966,622 - MMP_RS05080 0.37 -4.5 966,623 + MMP_RS05080 0.37 -4.7 966,623 + MMP_RS05080 0.37 -4.6 966,864 - MMP_RS05080 0.58 -5.3 966,910 + MMP_RS05080 0.62 -5.7 966,964 - MMP_RS05080 0.66 -5.3 966,964 - MMP_RS05080 0.66 -4.2 966,965 + MMP_RS05080 0.67 -5.6 966,965 + MMP_RS05080 0.67 -4.4 967,021 - MMP_RS05080 0.71 -6.4 967,022 + MMP_RS05080 0.72 -3.2
Or see this region's nucleotide sequence