Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02140 and MMP_RS02145 are separated by 69 nucleotides MMP_RS02145 and MMP_RS02150 are separated by 301 nucleotides
MMP_RS02140: MMP_RS02140 - methionine synthase, at 399,758 to 400,696
_RS02140
MMP_RS02145: MMP_RS02145 - winged helix-turn-helix transcriptional regulator, at 400,766 to 402,076
_RS02145
MMP_RS02150: MMP_RS02150 - hypothetical protein, at 402,378 to 403,463
_RS02150
Position (kb) 400
401
402
403 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 399.789 kb on - strand at 399.850 kb on - strand at 399.948 kb on - strand at 400.297 kb on - strand at 400.390 kb on - strand at 400.707 kb on + strand at 400.735 kb on - strand at 400.739 kb on - strand at 400.753 kb on + strand at 400.778 kb on + strand at 400.898 kb on - strand, within MMP_RS02145 at 400.906 kb on - strand, within MMP_RS02145 at 400.907 kb on + strand, within MMP_RS02145 at 400.912 kb on - strand, within MMP_RS02145 at 400.913 kb on + strand, within MMP_RS02145 at 401.009 kb on + strand, within MMP_RS02145 at 401.106 kb on - strand, within MMP_RS02145 at 401.107 kb on + strand, within MMP_RS02145 at 401.108 kb on - strand, within MMP_RS02145 at 401.109 kb on + strand, within MMP_RS02145 at 401.109 kb on + strand, within MMP_RS02145 at 401.109 kb on + strand, within MMP_RS02145 at 401.111 kb on + strand, within MMP_RS02145 at 401.113 kb on - strand, within MMP_RS02145 at 401.115 kb on - strand, within MMP_RS02145 at 401.116 kb on + strand, within MMP_RS02145 at 401.176 kb on + strand, within MMP_RS02145 at 401.213 kb on + strand, within MMP_RS02145 at 401.221 kb on - strand, within MMP_RS02145 at 401.221 kb on - strand, within MMP_RS02145 at 401.254 kb on + strand, within MMP_RS02145 at 401.300 kb on + strand, within MMP_RS02145 at 401.310 kb on + strand, within MMP_RS02145 at 401.326 kb on + strand, within MMP_RS02145 at 401.434 kb on - strand, within MMP_RS02145 at 401.527 kb on - strand, within MMP_RS02145 at 401.568 kb on - strand, within MMP_RS02145 at 401.584 kb on + strand, within MMP_RS02145 at 401.629 kb on + strand, within MMP_RS02145 at 401.672 kb on + strand, within MMP_RS02145 at 401.675 kb on + strand, within MMP_RS02145 at 401.680 kb on + strand, within MMP_RS02145 at 401.692 kb on + strand, within MMP_RS02145 at 401.869 kb on + strand, within MMP_RS02145 at 402.060 kb on - strand at 402.060 kb on - strand at 402.092 kb on + strand at 402.112 kb on + strand at 402.127 kb on - strand at 402.132 kb on - strand at 402.132 kb on - strand at 402.132 kb on - strand at 402.132 kb on - strand at 402.132 kb on - strand at 402.133 kb on + strand at 402.133 kb on + strand at 402.133 kb on + strand at 402.133 kb on + strand at 402.133 kb on + strand at 402.133 kb on + strand at 402.135 kb on + strand at 402.148 kb on - strand at 402.148 kb on - strand at 402.194 kb on + strand at 402.198 kb on - strand at 402.230 kb on + strand at 402.246 kb on + strand at 402.250 kb on - strand at 402.250 kb on - strand at 402.251 kb on + strand at 402.251 kb on + strand at 402.389 kb on - strand at 402.395 kb on - strand at 402.861 kb on - strand at 402.862 kb on + strand at 402.864 kb on + strand at 402.876 kb on - strand at 402.908 kb on - strand at 402.935 kb on + strand at 402.935 kb on + strand at 402.971 kb on + strand at 402.971 kb on + strand at 403.071 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE remove 399,789 - -3.2 399,850 - -2.1 399,948 - -4.8 400,297 - -4.9 400,390 - -3.7 400,707 + -1.3 400,735 - -1.7 400,739 - -2.1 400,753 + -1.3 400,778 + -2.3 400,898 - MMP_RS02145 0.10 -0.6 400,906 - MMP_RS02145 0.11 -1.6 400,907 + MMP_RS02145 0.11 -3.4 400,912 - MMP_RS02145 0.11 -3.5 400,913 + MMP_RS02145 0.11 -1.6 401,009 + MMP_RS02145 0.19 -1.3 401,106 - MMP_RS02145 0.26 -2.4 401,107 + MMP_RS02145 0.26 -1.3 401,108 - MMP_RS02145 0.26 -2.4 401,109 + MMP_RS02145 0.26 -2.0 401,109 + MMP_RS02145 0.26 -2.0 401,109 + MMP_RS02145 0.26 -3.0 401,111 + MMP_RS02145 0.26 -3.6 401,113 - MMP_RS02145 0.26 -1.9 401,115 - MMP_RS02145 0.27 -2.3 401,116 + MMP_RS02145 0.27 -2.2 401,176 + MMP_RS02145 0.31 -0.7 401,213 + MMP_RS02145 0.34 -2.9 401,221 - MMP_RS02145 0.35 -1.8 401,221 - MMP_RS02145 0.35 -2.0 401,254 + MMP_RS02145 0.37 -0.6 401,300 + MMP_RS02145 0.41 -1.9 401,310 + MMP_RS02145 0.41 -0.9 401,326 + MMP_RS02145 0.43 -2.7 401,434 - MMP_RS02145 0.51 -1.3 401,527 - MMP_RS02145 0.58 -3.0 401,568 - MMP_RS02145 0.61 -1.4 401,584 + MMP_RS02145 0.62 -0.9 401,629 + MMP_RS02145 0.66 -0.3 401,672 + MMP_RS02145 0.69 -1.6 401,675 + MMP_RS02145 0.69 -1.3 401,680 + MMP_RS02145 0.70 -3.2 401,692 + MMP_RS02145 0.71 -2.4 401,869 + MMP_RS02145 0.84 -2.7 402,060 - -0.2 402,060 - -0.1 402,092 + +0.0 402,112 + -3.2 402,127 - +0.3 402,132 - -0.2 402,132 - -0.7 402,132 - +0.1 402,132 - +0.1 402,132 - +0.2 402,133 + +0.3 402,133 + +0.1 402,133 + -0.0 402,133 + -0.3 402,133 + +0.4 402,133 + +0.3 402,135 + +0.2 402,148 - -0.3 402,148 - -0.2 402,194 + +0.1 402,198 - -2.9 402,230 + -0.6 402,246 + -1.4 402,250 - -3.3 402,250 - -3.4 402,251 + -0.1 402,251 + -1.6 402,389 - -3.0 402,395 - -0.6 402,861 - -2.8 402,862 + -2.0 402,864 + -2.2 402,876 - -1.0 402,908 - -1.1 402,935 + -1.3 402,935 + -3.2 402,971 + -1.8 402,971 + -0.2 403,071 - -2.3
Or see this region's nucleotide sequence