Strain Fitness in Methanococcus maripaludis S2 around MMP_RS02115

Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntMMP_RS02105 and MMP_RS02110 are separated by 15 nucleotidesMMP_RS02110 and MMP_RS02115 are separated by 241 nucleotidesMMP_RS02115 and MMP_RS02120 are separated by 201 nucleotides MMP_RS02105: MMP_RS02105 - uroporphyrinogen-III synthase, at 391,835 to 392,569 _RS02105 MMP_RS02110: MMP_RS02110 - hypothetical protein, at 392,585 to 392,863 _RS02110 MMP_RS02115: MMP_RS02115 - phosphopyruvate hydratase, at 393,105 to 394,397 _RS02115 MMP_RS02120: MMP_RS02120 - alanine--tRNA ligase, at 394,599 to 397,277 _RS02120 Position (kb) 393 394 395Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 392.169 kb on + strandat 392.433 kb on - strandat 392.616 kb on - strand, within MMP_RS02110at 392.616 kb on - strand, within MMP_RS02110at 392.876 kb on - strandat 392.896 kb on + strandat 392.910 kb on + strandat 392.920 kb on + strandat 393.010 kb on - strandat 393.515 kb on - strandat 393.541 kb on + strandat 393.926 kb on + strandat 393.937 kb on + strandat 394.320 kb on - strandat 394.401 kb on - strandat 394.406 kb on + strandat 394.443 kb on - strandat 394.453 kb on + strandat 394.479 kb on + strandat 394.491 kb on - strandat 394.577 kb on + strandat 394.620 kb on + strandat 394.779 kb on - strandat 394.780 kb on + strandat 395.033 kb on - strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE
remove
392,169 + -1.3
392,433 - -4.8
392,616 - MMP_RS02110 0.11 -3.1
392,616 - MMP_RS02110 0.11 -0.6
392,876 - -3.3
392,896 + -5.2
392,910 + -4.1
392,920 + +0.3
393,010 - -1.0
393,515 - -2.4
393,541 + -1.2
393,926 + -2.3
393,937 + -4.3
394,320 - -3.2
394,401 - +0.1
394,406 + -0.0
394,443 - -0.3
394,453 + -0.1
394,479 + +0.6
394,491 - -0.3
394,577 + -3.1
394,620 + -3.8
394,779 - -4.2
394,780 + -4.3
395,033 - -4.6

Or see this region's nucleotide sequence