Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01505 and MMP_RS01510 are separated by 20 nucleotides MMP_RS01510 and MMP_RS01515 are separated by 60 nucleotides MMP_RS01515 and MMP_RS01520 are separated by 1 nucleotides MMP_RS01520 and MMP_RS01525 are separated by 188 nucleotides
MMP_RS01505: MMP_RS01505 - hypothetical protein, at 280,059 to 281,036
_RS01505
MMP_RS01510: MMP_RS01510 - phosphoribosyl-AMP cyclohydrolase, at 281,057 to 281,446
_RS01510
MMP_RS01515: MMP_RS01515 - PINc/VapC family ATPase, at 281,507 to 283,399
_RS01515
MMP_RS01520: MMP_RS01520 - 5-(carboxyamino)imidazole ribonucleotide mutase, at 283,401 to 283,877
_RS01520
MMP_RS01525: MMP_RS01525 - nucleoside-diphosphate kinase, at 284,066 to 284,485
_RS01525
Position (kb) 281
282
283
284 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 280.934 kb on - strand at 280.991 kb on - strand at 280.991 kb on - strand at 280.993 kb on - strand at 280.994 kb on + strand at 281.301 kb on - strand at 281.374 kb on - strand at 281.375 kb on + strand at 281.375 kb on + strand at 281.617 kb on + strand at 281.742 kb on - strand, within MMP_RS01515 at 281.742 kb on - strand, within MMP_RS01515 at 281.767 kb on + strand, within MMP_RS01515 at 281.767 kb on + strand, within MMP_RS01515 at 281.770 kb on + strand, within MMP_RS01515 at 281.781 kb on + strand, within MMP_RS01515 at 281.924 kb on + strand, within MMP_RS01515 at 281.974 kb on + strand, within MMP_RS01515 at 282.159 kb on - strand, within MMP_RS01515 at 282.186 kb on - strand, within MMP_RS01515 at 282.546 kb on - strand, within MMP_RS01515 at 282.548 kb on - strand, within MMP_RS01515 at 282.549 kb on + strand, within MMP_RS01515 at 282.711 kb on - strand, within MMP_RS01515 at 282.820 kb on + strand, within MMP_RS01515 at 282.855 kb on - strand, within MMP_RS01515 at 282.856 kb on + strand, within MMP_RS01515 at 282.859 kb on - strand, within MMP_RS01515 at 282.859 kb on - strand, within MMP_RS01515 at 282.859 kb on - strand, within MMP_RS01515 at 282.860 kb on + strand, within MMP_RS01515 at 282.863 kb on - strand, within MMP_RS01515 at 283.111 kb on + strand, within MMP_RS01515 at 283.317 kb on - strand at 283.929 kb on - strand at 283.933 kb on - strand at 283.933 kb on - strand at 283.934 kb on + strand at 283.960 kb on + strand at 283.995 kb on - strand at 283.995 kb on - strand at 283.995 kb on - strand at 283.996 kb on + strand at 284.000 kb on - strand at 284.006 kb on + strand at 284.062 kb on - strand at 284.226 kb on + strand, within MMP_RS01525
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE remove 280,934 - -2.1 280,991 - -2.1 280,991 - -3.5 280,993 - -3.8 280,994 + -3.2 281,301 - -1.2 281,374 - -2.7 281,375 + -3.4 281,375 + -2.2 281,617 + +0.3 281,742 - MMP_RS01515 0.12 -0.2 281,742 - MMP_RS01515 0.12 +0.8 281,767 + MMP_RS01515 0.14 +0.3 281,767 + MMP_RS01515 0.14 +0.1 281,770 + MMP_RS01515 0.14 +0.3 281,781 + MMP_RS01515 0.14 -0.4 281,924 + MMP_RS01515 0.22 +0.8 281,974 + MMP_RS01515 0.25 +0.5 282,159 - MMP_RS01515 0.34 -0.1 282,186 - MMP_RS01515 0.36 -0.2 282,546 - MMP_RS01515 0.55 +0.0 282,548 - MMP_RS01515 0.55 +0.7 282,549 + MMP_RS01515 0.55 +0.1 282,711 - MMP_RS01515 0.64 +0.2 282,820 + MMP_RS01515 0.69 +0.8 282,855 - MMP_RS01515 0.71 +0.1 282,856 + MMP_RS01515 0.71 +1.6 282,859 - MMP_RS01515 0.71 -0.2 282,859 - MMP_RS01515 0.71 +0.4 282,859 - MMP_RS01515 0.71 +1.4 282,860 + MMP_RS01515 0.71 +0.5 282,863 - MMP_RS01515 0.72 +0.3 283,111 + MMP_RS01515 0.85 +0.3 283,317 - +0.4 283,929 - +0.5 283,933 - -0.0 283,933 - -0.2 283,934 + -1.5 283,960 + +0.4 283,995 - +0.2 283,995 - +0.7 283,995 - -0.0 283,996 + +0.4 284,000 - -0.9 284,006 + -0.3 284,062 - +0.1 284,226 + MMP_RS01525 0.38 +0.1
Or see this region's nucleotide sequence