Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00990 and MMP_RS00995 are separated by 135 nucleotides MMP_RS00995 and MMP_RS01000 are separated by 9 nucleotides MMP_RS01000 and MMP_RS01005 are separated by 174 nucleotides MMP_RS01005 and MMP_RS01010 are separated by 128 nucleotides
MMP_RS00990: MMP_RS00990 - hypothetical protein, at 186,183 to 186,542
_RS00990
MMP_RS00995: MMP_RS00995 - phosphoribosylformylglycinamidine synthase I, at 186,678 to 187,487
_RS00995
MMP_RS01000: MMP_RS01000 - AIR synthase related protein, at 187,497 to 190,466
_RS01000
MMP_RS01005: MMP_RS01005 - riboflavin synthase, at 190,641 to 191,111
_RS01005
MMP_RS01010: MMP_RS01010 - DUF4352 domain-containing protein, at 191,240 to 191,773
_RS01010
Position (kb) 187
188
189
190
191 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 186.505 kb on - strand, within MMP_RS00990 at 186.560 kb on - strand at 186.574 kb on + strand at 186.625 kb on + strand at 186.683 kb on - strand at 187.170 kb on - strand at 189.906 kb on - strand at 190.090 kb on + strand at 190.542 kb on - strand at 190.547 kb on - strand at 190.549 kb on - strand at 190.579 kb on - strand at 190.589 kb on + strand at 190.627 kb on + strand at 190.729 kb on - strand, within MMP_RS01005 at 190.730 kb on + strand, within MMP_RS01005 at 191.097 kb on - strand at 191.144 kb on - strand at 191.151 kb on - strand at 191.152 kb on + strand at 191.186 kb on - strand at 191.191 kb on - strand at 191.273 kb on - strand at 191.273 kb on - strand at 191.274 kb on + strand at 191.280 kb on - strand at 191.281 kb on + strand at 191.303 kb on + strand, within MMP_RS01010 at 191.309 kb on - strand, within MMP_RS01010 at 191.396 kb on - strand, within MMP_RS01010 at 191.397 kb on + strand, within MMP_RS01010 at 191.397 kb on + strand, within MMP_RS01010 at 191.442 kb on + strand, within MMP_RS01010
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE remove 186,505 - MMP_RS00990 0.89 -0.0 186,560 - -0.3 186,574 + -0.2 186,625 + -0.1 186,683 - -0.8 187,170 - -2.8 189,906 - -3.4 190,090 + -3.1 190,542 - -0.1 190,547 - -0.5 190,549 - +0.4 190,579 - -0.4 190,589 + -0.4 190,627 + +0.2 190,729 - MMP_RS01005 0.19 -3.8 190,730 + MMP_RS01005 0.19 -5.2 191,097 - -5.5 191,144 - -1.0 191,151 - +0.1 191,152 + -3.8 191,186 - +0.6 191,191 - +0.1 191,273 - +1.6 191,273 - +0.3 191,274 + -0.7 191,280 - -0.3 191,281 + -0.1 191,303 + MMP_RS01010 0.12 +0.4 191,309 - MMP_RS01010 0.13 -0.3 191,396 - MMP_RS01010 0.29 +0.1 191,397 + MMP_RS01010 0.29 +0.2 191,397 + MMP_RS01010 0.29 +0.0 191,442 + MMP_RS01010 0.38 -0.6
Or see this region's nucleotide sequence