Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12985 and BPHYT_RS12990 are separated by 63 nucleotides BPHYT_RS12990 and BPHYT_RS12995 are separated by 741 nucleotides
BPHYT_RS12985: BPHYT_RS12985 - amino acid acetyltransferase, at 2,969,554 to 2,970,942
_RS12985
BPHYT_RS12990: BPHYT_RS12990 - iron transporter, at 2,971,006 to 2,971,272
_RS12990
BPHYT_RS12995: BPHYT_RS12995 - MFS transporter, at 2,972,014 to 2,973,483
_RS12995
Position (kb)
2971
2972 Strain fitness (log2 ratio)
-1
0
1 at 2970.316 kb on + strand, within BPHYT_RS12985 at 2970.487 kb on + strand, within BPHYT_RS12985 at 2970.899 kb on - strand at 2970.950 kb on + strand at 2970.951 kb on - strand at 2970.951 kb on - strand at 2970.951 kb on - strand at 2970.951 kb on - strand at 2970.951 kb on - strand at 2970.954 kb on + strand at 2970.954 kb on + strand at 2970.955 kb on - strand at 2970.955 kb on - strand at 2970.955 kb on - strand at 2971.005 kb on + strand at 2971.005 kb on + strand at 2971.087 kb on + strand, within BPHYT_RS12990 at 2971.088 kb on - strand, within BPHYT_RS12990 at 2971.249 kb on + strand at 2971.249 kb on + strand at 2971.270 kb on + strand at 2971.270 kb on + strand at 2971.275 kb on + strand at 2971.275 kb on + strand at 2971.275 kb on + strand at 2971.276 kb on - strand at 2971.293 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,970,316 + BPHYT_RS12985 0.55 -0.9 2,970,487 + BPHYT_RS12985 0.67 -0.8 2,970,899 - -1.0 2,970,950 + -0.6 2,970,951 - +0.7 2,970,951 - -0.6 2,970,951 - -0.2 2,970,951 - +0.3 2,970,951 - +0.4 2,970,954 + -0.3 2,970,954 + +0.6 2,970,955 - -0.5 2,970,955 - -0.4 2,970,955 - -0.1 2,971,005 + -1.2 2,971,005 + +0.9 2,971,087 + BPHYT_RS12990 0.30 -1.5 2,971,088 - BPHYT_RS12990 0.31 -0.4 2,971,249 + -0.3 2,971,249 + -0.7 2,971,270 + -0.4 2,971,270 + +0.2 2,971,275 + -0.4 2,971,275 + +0.0 2,971,275 + +0.3 2,971,276 - +0.2 2,971,293 + +0.8
Or see this region's nucleotide sequence