Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09615 and BPHYT_RS09620 are separated by 78 nucleotides BPHYT_RS09620 and BPHYT_RS09625 overlap by 1 nucleotides BPHYT_RS09625 and BPHYT_RS09630 are separated by 94 nucleotides
BPHYT_RS09615: BPHYT_RS09615 - folate-binding protein, at 2,180,802 to 2,181,875
_RS09615
BPHYT_RS09620: BPHYT_RS09620 - aminodeoxychorismate lyase, at 2,181,954 to 2,182,964
_RS09620
BPHYT_RS09625: BPHYT_RS09625 - thymidylate kinase, at 2,182,964 to 2,183,584
_RS09625
BPHYT_RS09630: BPHYT_RS09630 - DNA polymerase III subunit delta', at 2,183,679 to 2,184,713
_RS09630
Position (kb)
2181
2182
2183 Strain fitness (log2 ratio)
-1
0
1 at 2181.926 kb on + strand at 2182.451 kb on + strand, within BPHYT_RS09620 at 2182.725 kb on + strand, within BPHYT_RS09620 at 2182.725 kb on + strand, within BPHYT_RS09620 at 2182.771 kb on - strand, within BPHYT_RS09620 at 2182.775 kb on + strand, within BPHYT_RS09620 at 2182.779 kb on + strand, within BPHYT_RS09620 at 2182.947 kb on + strand at 2183.609 kb on + strand at 2183.609 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,181,926 + -0.7 2,182,451 + BPHYT_RS09620 0.49 -0.7 2,182,725 + BPHYT_RS09620 0.76 -0.7 2,182,725 + BPHYT_RS09620 0.76 -0.6 2,182,771 - BPHYT_RS09620 0.81 -0.0 2,182,775 + BPHYT_RS09620 0.81 -1.6 2,182,779 + BPHYT_RS09620 0.82 -0.0 2,182,947 + -0.8 2,183,609 + +0.4 2,183,609 + +0.2
Or see this region's nucleotide sequence