Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc00424 and SMc00425 are separated by 134 nucleotides SMc00425 and SMc00461 overlap by 8 nucleotides SMc00461 and SMc00427 are separated by 98 nucleotides
SMc00424: SMc00424 - hypothetical protein, at 372,883 to 373,797
SMc00424
SMc00425: SMc00425 - transcriptional regulator, at 373,932 to 374,369
SMc00425
SMc00461: SMc00461 - deoxyuridine 5'-triphosphate nucleotidohydrolase, at 374,362 to 374,844
SMc00461
SMc00427: SMc00427 - peptide chain release factor RF-3 protein, at 374,943 to 376,526
SMc00427
Position (kb)
374
375 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6 at 373.496 kb on + strand, within SMc00424 at 373.496 kb on + strand, within SMc00424 at 373.496 kb on + strand, within SMc00424 at 373.496 kb on + strand, within SMc00424 at 373.497 kb on - strand, within SMc00424 at 373.497 kb on - strand, within SMc00424 at 373.746 kb on + strand at 373.747 kb on - strand at 373.783 kb on + strand at 373.783 kb on + strand at 373.784 kb on - strand at 374.165 kb on + strand, within SMc00425 at 374.165 kb on + strand, within SMc00425 at 374.165 kb on + strand, within SMc00425 at 374.363 kb on + strand at 374.364 kb on - strand at 374.368 kb on - strand at 374.368 kb on - strand at 374.368 kb on - strand at 374.652 kb on + strand, within SMc00461 at 374.653 kb on - strand, within SMc00461 at 374.858 kb on + strand at 374.859 kb on - strand at 374.859 kb on - strand at 374.909 kb on + strand at 375.238 kb on - strand, within SMc00427 at 375.396 kb on + strand, within SMc00427 at 375.487 kb on - strand, within SMc00427 at 375.487 kb on - strand, within SMc00427
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 373,496 + SMc00424 0.67 +0.1 373,496 + SMc00424 0.67 -0.4 373,496 + SMc00424 0.67 +0.0 373,496 + SMc00424 0.67 -0.8 373,497 - SMc00424 0.67 +0.6 373,497 - SMc00424 0.67 -1.2 373,746 + -0.2 373,747 - -0.3 373,783 + -0.2 373,783 + -0.9 373,784 - -1.5 374,165 + SMc00425 0.53 +1.6 374,165 + SMc00425 0.53 -0.8 374,165 + SMc00425 0.53 -0.3 374,363 + +0.1 374,364 - -0.1 374,368 - -1.0 374,368 - +1.9 374,368 - +1.2 374,652 + SMc00461 0.60 -0.6 374,653 - SMc00461 0.60 -0.5 374,858 + -0.6 374,859 - +0.4 374,859 - +0.4 374,909 + +0.5 375,238 - SMc00427 0.19 +6.3 375,396 + SMc00427 0.29 +5.3 375,487 - SMc00427 0.34 +5.2 375,487 - SMc00427 0.34 +5.2
Or see this region's nucleotide sequence