Experiment: Dimethyl Sulfoxide 6 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09160 and BPHYT_RS09165 are separated by 278 nucleotides BPHYT_RS09165 and BPHYT_RS09170 are separated by 80 nucleotides
BPHYT_RS09160: BPHYT_RS09160 - polyhydroxyalkanoate synthesis repressor PhaR, at 2,073,389 to 2,074,018
_RS09160
BPHYT_RS09165: BPHYT_RS09165 - tRNA-dihydrouridine synthase C, at 2,074,297 to 2,075,319
_RS09165
BPHYT_RS09170: BPHYT_RS09170 - ribosomal protein S12 methylthiotransferase RimO, at 2,075,400 to 2,076,785
_RS09170
Position (kb)
2074
2075
2076 Strain fitness (log2 ratio)
-1
0
1 at 2073.367 kb on - strand at 2073.386 kb on + strand at 2073.397 kb on + strand at 2073.397 kb on + strand at 2073.398 kb on - strand at 2073.474 kb on - strand, within BPHYT_RS09160 at 2073.474 kb on - strand, within BPHYT_RS09160 at 2073.474 kb on - strand, within BPHYT_RS09160 at 2073.650 kb on + strand, within BPHYT_RS09160 at 2073.684 kb on - strand, within BPHYT_RS09160 at 2073.752 kb on + strand, within BPHYT_RS09160 at 2073.836 kb on + strand, within BPHYT_RS09160 at 2073.836 kb on + strand, within BPHYT_RS09160 at 2073.837 kb on - strand, within BPHYT_RS09160 at 2073.837 kb on - strand, within BPHYT_RS09160 at 2074.016 kb on + strand at 2074.149 kb on + strand at 2074.149 kb on + strand at 2074.150 kb on - strand at 2074.339 kb on + strand at 2074.340 kb on - strand at 2074.340 kb on - strand at 2074.435 kb on + strand, within BPHYT_RS09165 at 2074.555 kb on - strand, within BPHYT_RS09165 at 2074.737 kb on + strand, within BPHYT_RS09165 at 2074.737 kb on + strand, within BPHYT_RS09165 at 2074.738 kb on - strand, within BPHYT_RS09165 at 2075.057 kb on + strand, within BPHYT_RS09165 at 2075.058 kb on - strand, within BPHYT_RS09165 at 2075.075 kb on - strand, within BPHYT_RS09165 at 2075.143 kb on + strand, within BPHYT_RS09165 at 2075.379 kb on + strand at 2076.040 kb on - strand, within BPHYT_RS09170
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 6 vol% remove 2,073,367 - -0.6 2,073,386 + +1.0 2,073,397 + -1.0 2,073,397 + -0.5 2,073,398 - +0.2 2,073,474 - BPHYT_RS09160 0.13 -1.2 2,073,474 - BPHYT_RS09160 0.13 -0.4 2,073,474 - BPHYT_RS09160 0.13 -0.1 2,073,650 + BPHYT_RS09160 0.41 -1.3 2,073,684 - BPHYT_RS09160 0.47 -0.6 2,073,752 + BPHYT_RS09160 0.58 +0.5 2,073,836 + BPHYT_RS09160 0.71 -0.8 2,073,836 + BPHYT_RS09160 0.71 +0.7 2,073,837 - BPHYT_RS09160 0.71 -1.5 2,073,837 - BPHYT_RS09160 0.71 -0.8 2,074,016 + -0.4 2,074,149 + +0.1 2,074,149 + -0.2 2,074,150 - -0.5 2,074,339 + +0.0 2,074,340 - -0.4 2,074,340 - -0.3 2,074,435 + BPHYT_RS09165 0.13 -0.4 2,074,555 - BPHYT_RS09165 0.25 -0.5 2,074,737 + BPHYT_RS09165 0.43 -0.6 2,074,737 + BPHYT_RS09165 0.43 -0.3 2,074,738 - BPHYT_RS09165 0.43 +0.2 2,075,057 + BPHYT_RS09165 0.74 -0.2 2,075,058 - BPHYT_RS09165 0.74 -0.5 2,075,075 - BPHYT_RS09165 0.76 +0.2 2,075,143 + BPHYT_RS09165 0.83 -0.1 2,075,379 + +0.2 2,076,040 - BPHYT_RS09170 0.46 +0.8
Or see this region's nucleotide sequence