Experiment: Dimethyl Sulfoxide 6 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS06975 and BPHYT_RS06980 are separated by 143 nucleotides BPHYT_RS06980 and BPHYT_RS06985 are separated by 189 nucleotides BPHYT_RS06985 and BPHYT_RS06990 are separated by 55 nucleotides BPHYT_RS06990 and BPHYT_RS06995 are separated by 348 nucleotides
BPHYT_RS06975: BPHYT_RS06975 - glutamate racemase, at 1,576,632 to 1,577,519
_RS06975
BPHYT_RS06980: BPHYT_RS06980 - bacterioferritin, at 1,577,663 to 1,578,142
_RS06980
BPHYT_RS06985: BPHYT_RS06985 - fumarate hydratase, at 1,578,332 to 1,579,855
_RS06985
BPHYT_RS06990: BPHYT_RS06990 - membrane protein, at 1,579,911 to 1,580,480
_RS06990
BPHYT_RS06995: BPHYT_RS06995 - multidrug DMT transporter permease, at 1,580,829 to 1,581,689
_RS06995
Position (kb) 1578
1579
1580 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1577.371 kb on + strand, within BPHYT_RS06975 at 1577.372 kb on - strand, within BPHYT_RS06975 at 1577.372 kb on - strand, within BPHYT_RS06975 at 1577.520 kb on - strand at 1577.585 kb on + strand at 1577.585 kb on + strand at 1577.585 kb on + strand at 1577.585 kb on + strand at 1577.586 kb on - strand at 1577.586 kb on - strand at 1577.587 kb on + strand at 1577.618 kb on - strand at 1577.618 kb on - strand at 1577.697 kb on + strand at 1577.698 kb on - strand at 1577.698 kb on - strand at 1577.824 kb on - strand, within BPHYT_RS06980 at 1578.010 kb on - strand, within BPHYT_RS06980 at 1578.046 kb on + strand, within BPHYT_RS06980 at 1578.046 kb on + strand, within BPHYT_RS06980 at 1578.046 kb on + strand, within BPHYT_RS06980 at 1578.047 kb on - strand, within BPHYT_RS06980 at 1578.047 kb on - strand, within BPHYT_RS06980 at 1578.048 kb on + strand, within BPHYT_RS06980 at 1578.049 kb on - strand, within BPHYT_RS06980 at 1578.070 kb on - strand, within BPHYT_RS06980 at 1578.155 kb on + strand at 1578.155 kb on + strand at 1578.328 kb on + strand at 1578.456 kb on + strand at 1578.456 kb on + strand at 1578.456 kb on + strand at 1578.457 kb on - strand at 1578.457 kb on - strand at 1578.525 kb on + strand, within BPHYT_RS06985 at 1578.526 kb on - strand, within BPHYT_RS06985 at 1578.552 kb on + strand, within BPHYT_RS06985 at 1578.553 kb on - strand, within BPHYT_RS06985 at 1578.787 kb on + strand, within BPHYT_RS06985 at 1579.549 kb on - strand, within BPHYT_RS06985 at 1579.749 kb on + strand at 1579.749 kb on + strand at 1579.773 kb on + strand at 1579.788 kb on + strand at 1579.789 kb on - strand at 1579.791 kb on + strand at 1579.791 kb on + strand at 1579.792 kb on - strand at 1579.800 kb on + strand at 1579.801 kb on - strand at 1579.859 kb on + strand at 1579.859 kb on + strand at 1579.860 kb on - strand at 1579.860 kb on - strand at 1579.860 kb on - strand at 1580.081 kb on + strand, within BPHYT_RS06990 at 1580.395 kb on + strand, within BPHYT_RS06990 at 1580.425 kb on + strand at 1580.564 kb on - strand at 1580.613 kb on + strand at 1580.711 kb on - strand at 1580.711 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 6 vol% remove 1,577,371 + BPHYT_RS06975 0.83 -1.0 1,577,372 - BPHYT_RS06975 0.83 -0.5 1,577,372 - BPHYT_RS06975 0.83 +0.5 1,577,520 - -0.1 1,577,585 + +0.2 1,577,585 + -0.5 1,577,585 + -0.7 1,577,585 + -0.9 1,577,586 - -0.9 1,577,586 - +0.5 1,577,587 + +0.3 1,577,618 - +0.3 1,577,618 - +0.9 1,577,697 + +1.0 1,577,698 - +1.5 1,577,698 - +1.9 1,577,824 - BPHYT_RS06980 0.34 +0.1 1,578,010 - BPHYT_RS06980 0.72 +2.5 1,578,046 + BPHYT_RS06980 0.80 -0.5 1,578,046 + BPHYT_RS06980 0.80 -0.8 1,578,046 + BPHYT_RS06980 0.80 +0.1 1,578,047 - BPHYT_RS06980 0.80 +1.0 1,578,047 - BPHYT_RS06980 0.80 -0.1 1,578,048 + BPHYT_RS06980 0.80 +1.2 1,578,049 - BPHYT_RS06980 0.80 +0.0 1,578,070 - BPHYT_RS06980 0.85 -1.0 1,578,155 + +1.0 1,578,155 + -0.4 1,578,328 + +0.3 1,578,456 + -0.8 1,578,456 + -1.2 1,578,456 + -0.4 1,578,457 - -1.0 1,578,457 - -1.0 1,578,525 + BPHYT_RS06985 0.13 -1.4 1,578,526 - BPHYT_RS06985 0.13 -1.1 1,578,552 + BPHYT_RS06985 0.14 -0.8 1,578,553 - BPHYT_RS06985 0.15 -0.5 1,578,787 + BPHYT_RS06985 0.30 -0.5 1,579,549 - BPHYT_RS06985 0.80 -0.6 1,579,749 + -0.7 1,579,749 + -0.9 1,579,773 + -1.6 1,579,788 + -2.1 1,579,789 - -1.2 1,579,791 + +0.9 1,579,791 + -0.6 1,579,792 - -0.1 1,579,800 + -2.1 1,579,801 - -0.6 1,579,859 + -0.9 1,579,859 + -1.1 1,579,860 - -0.1 1,579,860 - -0.4 1,579,860 - -0.8 1,580,081 + BPHYT_RS06990 0.30 -2.9 1,580,395 + BPHYT_RS06990 0.85 -0.1 1,580,425 + -1.2 1,580,564 - -0.8 1,580,613 + -1.0 1,580,711 - +0.9 1,580,711 - -0.1
Or see this region's nucleotide sequence