Experiment: Dimethyl Sulfoxide 6 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00705 and BPHYT_RS00710 overlap by 14 nucleotides BPHYT_RS00710 and BPHYT_RS00715 are separated by 18 nucleotides BPHYT_RS00715 and BPHYT_RS00720 are separated by 54 nucleotides
BPHYT_RS00705: BPHYT_RS00705 - branched-chain amino acid ABC transporter substrate-binding protein, at 164,854 to 165,588
_RS00705
BPHYT_RS00710: BPHYT_RS00710 - branched-chain amino acid ABC transporter ATPase, at 165,575 to 166,318
_RS00710
BPHYT_RS00715: BPHYT_RS00715 - branched-chain amino acid ABC transporter substrate-binding protein, at 166,337 to 167,515
_RS00715
BPHYT_RS00720: BPHYT_RS00720 - amidase, at 167,570 to 168,997
_RS00720
Position (kb) 166
167
168 Strain fitness (log2 ratio) -1
0
1 at 165.515 kb on - strand at 165.553 kb on + strand at 165.553 kb on + strand at 165.554 kb on - strand at 165.912 kb on + strand, within BPHYT_RS00710 at 165.912 kb on + strand, within BPHYT_RS00710 at 165.920 kb on + strand, within BPHYT_RS00710 at 166.262 kb on + strand at 166.262 kb on + strand at 166.648 kb on + strand, within BPHYT_RS00715 at 167.157 kb on - strand, within BPHYT_RS00715 at 167.205 kb on - strand, within BPHYT_RS00715 at 167.206 kb on + strand, within BPHYT_RS00715 at 167.206 kb on + strand, within BPHYT_RS00715 at 167.216 kb on + strand, within BPHYT_RS00715 at 167.283 kb on - strand, within BPHYT_RS00715 at 167.517 kb on + strand at 167.517 kb on + strand at 167.517 kb on + strand at 167.659 kb on - strand at 168.492 kb on - strand, within BPHYT_RS00720 at 168.495 kb on - strand, within BPHYT_RS00720
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 6 vol% remove 165,515 - -0.1 165,553 + +0.9 165,553 + -1.4 165,554 - -0.2 165,912 + BPHYT_RS00710 0.45 +0.7 165,912 + BPHYT_RS00710 0.45 +0.2 165,920 + BPHYT_RS00710 0.46 +0.2 166,262 + +0.1 166,262 + +0.1 166,648 + BPHYT_RS00715 0.26 -0.5 167,157 - BPHYT_RS00715 0.70 +0.2 167,205 - BPHYT_RS00715 0.74 +0.5 167,206 + BPHYT_RS00715 0.74 -0.2 167,206 + BPHYT_RS00715 0.74 -0.3 167,216 + BPHYT_RS00715 0.75 -0.2 167,283 - BPHYT_RS00715 0.80 -0.4 167,517 + +0.6 167,517 + +0.1 167,517 + -0.3 167,659 - +0.8 168,492 - BPHYT_RS00720 0.65 +0.5 168,495 - BPHYT_RS00720 0.65 -0.1
Or see this region's nucleotide sequence