Experiment: Vancomycin 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_87 and Pf6N2E2_88 are separated by 175 nucleotides
Pf6N2E2_87 - hypothetical protein, at 131,038 to 131,397
_87
Pf6N2E2_88 - hypothetical protein, at 131,573 to 131,746
_88
Position (kb) 131
132 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 130.587 kb on + strand at 130.587 kb on + strand at 130.587 kb on + strand at 130.588 kb on - strand at 130.613 kb on - strand at 130.743 kb on + strand at 130.743 kb on + strand at 130.744 kb on - strand at 130.754 kb on + strand at 130.754 kb on + strand at 130.754 kb on + strand at 130.755 kb on - strand at 130.755 kb on - strand at 130.755 kb on - strand at 130.764 kb on - strand at 130.812 kb on + strand at 130.812 kb on + strand at 130.812 kb on + strand at 130.812 kb on + strand at 130.813 kb on - strand at 130.875 kb on + strand at 130.892 kb on + strand at 130.892 kb on + strand at 131.004 kb on + strand at 131.097 kb on + strand, within Pf6N2E2_87 at 131.242 kb on + strand, within Pf6N2E2_87 at 131.243 kb on - strand, within Pf6N2E2_87 at 131.284 kb on + strand, within Pf6N2E2_87 at 131.284 kb on + strand, within Pf6N2E2_87 at 131.284 kb on + strand, within Pf6N2E2_87 at 131.285 kb on - strand, within Pf6N2E2_87 at 131.285 kb on - strand, within Pf6N2E2_87 at 131.285 kb on - strand, within Pf6N2E2_87 at 131.285 kb on - strand, within Pf6N2E2_87 at 131.395 kb on + strand at 131.618 kb on + strand, within Pf6N2E2_88 at 131.687 kb on + strand, within Pf6N2E2_88 at 131.739 kb on - strand at 131.739 kb on - strand at 131.742 kb on + strand at 131.742 kb on + strand at 131.742 kb on + strand at 131.743 kb on - strand at 131.743 kb on - strand at 131.743 kb on - strand at 131.743 kb on - strand at 131.743 kb on - strand at 131.743 kb on - strand at 131.804 kb on + strand at 131.805 kb on - strand at 132.092 kb on - strand at 132.161 kb on + strand at 132.239 kb on - strand at 132.239 kb on - strand at 132.261 kb on + strand at 132.262 kb on - strand at 132.284 kb on + strand at 132.284 kb on + strand at 132.338 kb on + strand at 132.392 kb on + strand at 132.393 kb on - strand at 132.393 kb on - strand at 132.397 kb on - strand at 132.421 kb on + strand at 132.421 kb on + strand at 132.421 kb on + strand at 132.421 kb on + strand at 132.421 kb on + strand at 132.422 kb on - strand at 132.422 kb on - strand at 132.422 kb on - strand at 132.422 kb on - strand at 132.589 kb on + strand at 132.589 kb on + strand at 132.609 kb on + strand at 132.609 kb on + strand at 132.609 kb on + strand at 132.609 kb on + strand at 132.609 kb on + strand at 132.609 kb on + strand at 132.609 kb on + strand at 132.610 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.12 mg/ml remove 130,587 + -0.3 130,587 + -1.4 130,587 + -0.0 130,588 - -0.1 130,613 - +0.6 130,743 + +0.8 130,743 + +0.1 130,744 - +0.6 130,754 + -1.5 130,754 + +0.9 130,754 + +0.3 130,755 - +0.4 130,755 - -2.3 130,755 - +0.6 130,764 - +0.4 130,812 + +0.6 130,812 + -0.2 130,812 + +0.1 130,812 + +0.5 130,813 - -0.6 130,875 + -0.2 130,892 + +0.3 130,892 + +0.4 131,004 + +0.5 131,097 + Pf6N2E2_87 0.16 +0.0 131,242 + Pf6N2E2_87 0.57 -0.3 131,243 - Pf6N2E2_87 0.57 -0.2 131,284 + Pf6N2E2_87 0.68 -0.9 131,284 + Pf6N2E2_87 0.68 +0.7 131,284 + Pf6N2E2_87 0.68 +0.1 131,285 - Pf6N2E2_87 0.69 -0.0 131,285 - Pf6N2E2_87 0.69 -0.5 131,285 - Pf6N2E2_87 0.69 -0.0 131,285 - Pf6N2E2_87 0.69 +0.5 131,395 + +0.6 131,618 + Pf6N2E2_88 0.26 +1.0 131,687 + Pf6N2E2_88 0.66 +0.3 131,739 - -0.3 131,739 - +0.0 131,742 + +0.8 131,742 + +0.3 131,742 + +0.1 131,743 - +0.4 131,743 - +0.0 131,743 - +0.2 131,743 - +0.8 131,743 - -3.0 131,743 - -0.3 131,804 + -0.2 131,805 - +0.0 132,092 - +0.3 132,161 + +0.1 132,239 - -1.2 132,239 - +0.5 132,261 + +0.0 132,262 - +0.4 132,284 + -0.4 132,284 + -1.0 132,338 + +1.2 132,392 + +0.6 132,393 - +0.4 132,393 - +0.5 132,397 - -0.2 132,421 + -1.1 132,421 + +0.1 132,421 + +1.2 132,421 + -1.4 132,421 + -0.7 132,422 - -0.7 132,422 - +0.1 132,422 - +0.7 132,422 - -0.4 132,589 + -2.4 132,589 + -0.2 132,609 + -0.3 132,609 + +1.3 132,609 + -0.2 132,609 + +0.5 132,609 + +0.5 132,609 + +1.1 132,609 + -0.2 132,610 - -0.1
Or see this region's nucleotide sequence