Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_586

Experiment: Vancomycin 0.12 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_585 and Pf6N2E2_586 are separated by 267 nucleotidesPf6N2E2_586 and Pf6N2E2_587 are separated by 147 nucleotidesPf6N2E2_587 and Pf6N2E2_588 are separated by 65 nucleotides Pf6N2E2_585 - Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1), at 661,392 to 663,206 _585 Pf6N2E2_586 - Membrane-bound lytic murein transglycosylase D precursor (EC 3.2.1.-), at 663,474 to 664,874 _586 Pf6N2E2_587 - Hydroxyacylglutathione hydrolase (EC 3.1.2.6), at 665,022 to 665,789 _587 Pf6N2E2_588 - FIG005121: SAM-dependent methyltransferase (EC 2.1.1.-), at 665,855 to 666,613 _588 Position (kb) 663 664 665Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 662.706 kb on + strand, within Pf6N2E2_585at 662.782 kb on + strand, within Pf6N2E2_585at 662.782 kb on + strand, within Pf6N2E2_585at 662.887 kb on + strand, within Pf6N2E2_585at 662.887 kb on + strand, within Pf6N2E2_585at 662.887 kb on + strand, within Pf6N2E2_585at 663.016 kb on + strand, within Pf6N2E2_585at 663.142 kb on + strandat 663.219 kb on + strandat 663.219 kb on + strandat 663.219 kb on + strandat 663.219 kb on + strandat 663.220 kb on - strandat 663.220 kb on - strandat 663.220 kb on - strandat 663.257 kb on + strandat 663.266 kb on + strandat 663.266 kb on + strandat 663.267 kb on - strandat 663.267 kb on - strandat 663.271 kb on + strandat 663.281 kb on + strandat 663.391 kb on + strandat 663.488 kb on - strandat 663.586 kb on + strandat 663.586 kb on + strandat 663.586 kb on + strandat 663.587 kb on - strandat 663.587 kb on - strandat 663.587 kb on - strandat 663.587 kb on - strandat 663.587 kb on - strandat 663.613 kb on + strandat 663.614 kb on - strand, within Pf6N2E2_586at 663.614 kb on - strand, within Pf6N2E2_586at 663.614 kb on - strand, within Pf6N2E2_586at 663.826 kb on + strand, within Pf6N2E2_586at 664.139 kb on - strand, within Pf6N2E2_586at 664.189 kb on + strand, within Pf6N2E2_586at 664.189 kb on + strand, within Pf6N2E2_586at 664.189 kb on + strand, within Pf6N2E2_586at 664.190 kb on - strand, within Pf6N2E2_586at 664.190 kb on - strand, within Pf6N2E2_586at 664.190 kb on - strand, within Pf6N2E2_586at 664.190 kb on - strand, within Pf6N2E2_586at 664.190 kb on - strand, within Pf6N2E2_586at 664.190 kb on - strand, within Pf6N2E2_586at 664.190 kb on - strand, within Pf6N2E2_586at 664.295 kb on - strand, within Pf6N2E2_586at 664.352 kb on - strand, within Pf6N2E2_586at 664.399 kb on + strandat 664.399 kb on + strandat 664.400 kb on - strand, within Pf6N2E2_586at 664.400 kb on - strand, within Pf6N2E2_586at 664.427 kb on - strand, within Pf6N2E2_586at 664.480 kb on + strand, within Pf6N2E2_586at 664.495 kb on + strand, within Pf6N2E2_586at 664.567 kb on + strand, within Pf6N2E2_586at 664.568 kb on - strand, within Pf6N2E2_586at 664.568 kb on - strand, within Pf6N2E2_586at 664.568 kb on - strand, within Pf6N2E2_586at 664.568 kb on - strand, within Pf6N2E2_586at 664.576 kb on + strand, within Pf6N2E2_586at 664.576 kb on + strand, within Pf6N2E2_586at 664.933 kb on + strandat 665.004 kb on + strandat 665.023 kb on + strandat 665.023 kb on + strandat 665.024 kb on - strandat 665.030 kb on + strandat 665.030 kb on + strandat 665.031 kb on - strandat 665.031 kb on - strandat 665.282 kb on + strand, within Pf6N2E2_587at 665.282 kb on + strand, within Pf6N2E2_587at 665.282 kb on + strand, within Pf6N2E2_587at 665.282 kb on + strand, within Pf6N2E2_587at 665.283 kb on - strand, within Pf6N2E2_587at 665.284 kb on + strand, within Pf6N2E2_587at 665.284 kb on + strand, within Pf6N2E2_587at 665.284 kb on + strand, within Pf6N2E2_587at 665.284 kb on + strand, within Pf6N2E2_587at 665.284 kb on + strand, within Pf6N2E2_587at 665.284 kb on + strand, within Pf6N2E2_587at 665.284 kb on + strand, within Pf6N2E2_587at 665.284 kb on + strand, within Pf6N2E2_587at 665.285 kb on - strand, within Pf6N2E2_587at 665.285 kb on - strand, within Pf6N2E2_587at 665.299 kb on + strand, within Pf6N2E2_587at 665.299 kb on + strand, within Pf6N2E2_587at 665.300 kb on - strand, within Pf6N2E2_587at 665.310 kb on - strand, within Pf6N2E2_587at 665.431 kb on + strand, within Pf6N2E2_587at 665.431 kb on + strand, within Pf6N2E2_587at 665.431 kb on + strand, within Pf6N2E2_587at 665.434 kb on + strand, within Pf6N2E2_587at 665.470 kb on + strand, within Pf6N2E2_587at 665.470 kb on + strand, within Pf6N2E2_587at 665.736 kb on + strandat 665.749 kb on + strandat 665.749 kb on + strandat 665.749 kb on + strandat 665.749 kb on + strandat 665.749 kb on + strandat 665.749 kb on + strandat 665.750 kb on - strandat 665.750 kb on - strandat 665.750 kb on - strandat 665.750 kb on - strandat 665.785 kb on - strandat 665.785 kb on - strandat 665.785 kb on - strandat 665.826 kb on + strandat 665.826 kb on + strandat 665.827 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Vancomycin 0.12 mg/ml
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662,706 + Pf6N2E2_585 0.72 +2.3
662,782 + Pf6N2E2_585 0.77 -1.4
662,782 + Pf6N2E2_585 0.77 -0.7
662,887 + Pf6N2E2_585 0.82 -0.3
662,887 + Pf6N2E2_585 0.82 +0.2
662,887 + Pf6N2E2_585 0.82 -0.6
663,016 + Pf6N2E2_585 0.89 +0.9
663,142 + -1.6
663,219 + -0.5
663,219 + +0.2
663,219 + -0.4
663,219 + -0.9
663,220 - +0.4
663,220 - -0.6
663,220 - -0.2
663,257 + +0.2
663,266 + -0.0
663,266 + -1.5
663,267 - -0.9
663,267 - -0.2
663,271 + +0.9
663,281 + -0.5
663,391 + -0.6
663,488 - -2.8
663,586 + -0.2
663,586 + +3.0
663,586 + -0.3
663,587 - -0.1
663,587 - +0.9
663,587 - +0.4
663,587 - +0.7
663,587 - -0.3
663,613 + -0.4
663,614 - Pf6N2E2_586 0.10 +0.5
663,614 - Pf6N2E2_586 0.10 -0.9
663,614 - Pf6N2E2_586 0.10 -1.4
663,826 + Pf6N2E2_586 0.25 -1.0
664,139 - Pf6N2E2_586 0.47 -1.9
664,189 + Pf6N2E2_586 0.51 -2.1
664,189 + Pf6N2E2_586 0.51 -1.1
664,189 + Pf6N2E2_586 0.51 -0.9
664,190 - Pf6N2E2_586 0.51 -1.6
664,190 - Pf6N2E2_586 0.51 +0.5
664,190 - Pf6N2E2_586 0.51 -1.0
664,190 - Pf6N2E2_586 0.51 -1.6
664,190 - Pf6N2E2_586 0.51 -1.1
664,190 - Pf6N2E2_586 0.51 +0.4
664,190 - Pf6N2E2_586 0.51 -1.9
664,295 - Pf6N2E2_586 0.59 +0.0
664,352 - Pf6N2E2_586 0.63 -2.5
664,399 + +0.1
664,399 + +0.7
664,400 - Pf6N2E2_586 0.66 -1.8
664,400 - Pf6N2E2_586 0.66 -2.1
664,427 - Pf6N2E2_586 0.68 -2.0
664,480 + Pf6N2E2_586 0.72 -1.5
664,495 + Pf6N2E2_586 0.73 -2.8
664,567 + Pf6N2E2_586 0.78 -2.8
664,568 - Pf6N2E2_586 0.78 -1.2
664,568 - Pf6N2E2_586 0.78 -1.9
664,568 - Pf6N2E2_586 0.78 -0.9
664,568 - Pf6N2E2_586 0.78 -0.8
664,576 + Pf6N2E2_586 0.79 -3.8
664,576 + Pf6N2E2_586 0.79 -0.5
664,933 + -2.6
665,004 + -0.0
665,023 + -0.8
665,023 + -0.5
665,024 - -0.4
665,030 + -0.7
665,030 + +0.2
665,031 - -0.7
665,031 - +0.4
665,282 + Pf6N2E2_587 0.34 -1.0
665,282 + Pf6N2E2_587 0.34 +0.7
665,282 + Pf6N2E2_587 0.34 -0.4
665,282 + Pf6N2E2_587 0.34 -0.6
665,283 - Pf6N2E2_587 0.34 +1.3
665,284 + Pf6N2E2_587 0.34 -2.1
665,284 + Pf6N2E2_587 0.34 -0.0
665,284 + Pf6N2E2_587 0.34 -0.1
665,284 + Pf6N2E2_587 0.34 -0.2
665,284 + Pf6N2E2_587 0.34 +0.4
665,284 + Pf6N2E2_587 0.34 -0.9
665,284 + Pf6N2E2_587 0.34 -0.8
665,284 + Pf6N2E2_587 0.34 -1.4
665,285 - Pf6N2E2_587 0.34 -0.0
665,285 - Pf6N2E2_587 0.34 +0.8
665,299 + Pf6N2E2_587 0.36 -0.2
665,299 + Pf6N2E2_587 0.36 +0.8
665,300 - Pf6N2E2_587 0.36 -0.6
665,310 - Pf6N2E2_587 0.38 -0.5
665,431 + Pf6N2E2_587 0.53 +0.2
665,431 + Pf6N2E2_587 0.53 -0.5
665,431 + Pf6N2E2_587 0.53 +0.8
665,434 + Pf6N2E2_587 0.54 -0.8
665,470 + Pf6N2E2_587 0.58 +0.1
665,470 + Pf6N2E2_587 0.58 -1.9
665,736 + +0.5
665,749 + -0.1
665,749 + -0.3
665,749 + -0.6
665,749 + +0.8
665,749 + -1.8
665,749 + -0.6
665,750 - +0.2
665,750 - +0.7
665,750 - +0.5
665,750 - +1.9
665,785 - -0.3
665,785 - +1.0
665,785 - +0.5
665,826 + +1.4
665,826 + +0.2
665,827 - +0.2

Or see this region's nucleotide sequence