Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_417

Experiment: Vancomycin 0.12 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_415 and Pf6N2E2_416 overlap by 4 nucleotidesPf6N2E2_416 and Pf6N2E2_417 are separated by 39 nucleotidesPf6N2E2_417 and Pf6N2E2_418 are separated by 33 nucleotides Pf6N2E2_415 - Chemotaxis protein methyltransferase CheR (EC 2.1.1.80), at 482,939 to 483,775 _415 Pf6N2E2_416 - Positive regulator of CheA protein activity (CheW), at 483,772 to 484,308 _416 Pf6N2E2_417 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 484,348 to 486,006 _417 Pf6N2E2_418 - Signal transduction histidine kinase CheA (EC 2.7.3.-), at 486,040 to 488,142 _418 Position (kb) 484 485 486 487Strain fitness (log2 ratio) -2 -1 0 1 2 3at 483.393 kb on + strand, within Pf6N2E2_415at 483.394 kb on - strand, within Pf6N2E2_415at 483.465 kb on - strand, within Pf6N2E2_415at 483.465 kb on - strand, within Pf6N2E2_415at 483.548 kb on + strand, within Pf6N2E2_415at 483.548 kb on + strand, within Pf6N2E2_415at 483.548 kb on + strand, within Pf6N2E2_415at 483.548 kb on + strand, within Pf6N2E2_415at 483.548 kb on + strand, within Pf6N2E2_415at 483.548 kb on + strand, within Pf6N2E2_415at 483.549 kb on - strand, within Pf6N2E2_415at 483.549 kb on - strand, within Pf6N2E2_415at 483.549 kb on - strand, within Pf6N2E2_415at 483.588 kb on + strand, within Pf6N2E2_415at 483.597 kb on + strand, within Pf6N2E2_415at 483.597 kb on + strand, within Pf6N2E2_415at 483.598 kb on - strand, within Pf6N2E2_415at 483.612 kb on + strand, within Pf6N2E2_415at 483.613 kb on - strand, within Pf6N2E2_415at 483.691 kb on - strand, within Pf6N2E2_415at 483.691 kb on - strand, within Pf6N2E2_415at 483.859 kb on + strand, within Pf6N2E2_416at 483.859 kb on + strand, within Pf6N2E2_416at 484.239 kb on + strand, within Pf6N2E2_416at 484.239 kb on + strand, within Pf6N2E2_416at 484.240 kb on - strand, within Pf6N2E2_416at 484.240 kb on - strand, within Pf6N2E2_416at 484.240 kb on - strand, within Pf6N2E2_416at 484.251 kb on - strand, within Pf6N2E2_416at 484.251 kb on - strand, within Pf6N2E2_416at 484.324 kb on - strandat 484.324 kb on - strandat 484.324 kb on - strandat 484.394 kb on + strandat 484.394 kb on + strandat 484.395 kb on - strandat 485.630 kb on + strand, within Pf6N2E2_417at 485.631 kb on - strand, within Pf6N2E2_417at 485.700 kb on - strand, within Pf6N2E2_417at 485.810 kb on + strand, within Pf6N2E2_417at 485.810 kb on + strand, within Pf6N2E2_417at 485.811 kb on - strand, within Pf6N2E2_417at 485.985 kb on + strandat 485.985 kb on + strandat 485.986 kb on - strandat 485.986 kb on - strandat 485.986 kb on - strandat 485.993 kb on + strandat 485.993 kb on + strandat 485.993 kb on + strandat 485.993 kb on + strandat 485.993 kb on + strandat 485.994 kb on - strandat 485.994 kb on - strandat 485.994 kb on - strandat 486.014 kb on + strandat 486.014 kb on + strandat 486.014 kb on + strandat 486.014 kb on + strandat 486.015 kb on - strandat 486.034 kb on + strandat 486.061 kb on + strandat 486.062 kb on - strandat 486.371 kb on + strand, within Pf6N2E2_418at 486.371 kb on + strand, within Pf6N2E2_418at 486.449 kb on + strand, within Pf6N2E2_418at 486.449 kb on + strand, within Pf6N2E2_418at 486.449 kb on + strand, within Pf6N2E2_418at 486.449 kb on + strand, within Pf6N2E2_418at 486.449 kb on + strand, within Pf6N2E2_418at 486.449 kb on + strand, within Pf6N2E2_418at 486.450 kb on - strand, within Pf6N2E2_418at 486.450 kb on - strand, within Pf6N2E2_418at 486.450 kb on - strand, within Pf6N2E2_418at 486.450 kb on - strand, within Pf6N2E2_418at 486.450 kb on - strand, within Pf6N2E2_418at 486.450 kb on - strand, within Pf6N2E2_418at 486.450 kb on - strand, within Pf6N2E2_418at 486.668 kb on + strand, within Pf6N2E2_418at 486.668 kb on + strand, within Pf6N2E2_418at 486.668 kb on + strand, within Pf6N2E2_418at 486.669 kb on - strand, within Pf6N2E2_418at 486.669 kb on - strand, within Pf6N2E2_418

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Per-strain Table

Position Strand Gene LocusTag Fraction Vancomycin 0.12 mg/ml
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483,393 + Pf6N2E2_415 0.54 +0.5
483,394 - Pf6N2E2_415 0.54 +0.2
483,465 - Pf6N2E2_415 0.63 +0.6
483,465 - Pf6N2E2_415 0.63 -0.6
483,548 + Pf6N2E2_415 0.73 +0.1
483,548 + Pf6N2E2_415 0.73 -2.2
483,548 + Pf6N2E2_415 0.73 +0.6
483,548 + Pf6N2E2_415 0.73 -0.2
483,548 + Pf6N2E2_415 0.73 +0.1
483,548 + Pf6N2E2_415 0.73 +0.9
483,549 - Pf6N2E2_415 0.73 -0.4
483,549 - Pf6N2E2_415 0.73 +0.6
483,549 - Pf6N2E2_415 0.73 +0.4
483,588 + Pf6N2E2_415 0.78 +0.5
483,597 + Pf6N2E2_415 0.79 -0.7
483,597 + Pf6N2E2_415 0.79 +1.0
483,598 - Pf6N2E2_415 0.79 +0.4
483,612 + Pf6N2E2_415 0.80 -0.4
483,613 - Pf6N2E2_415 0.81 -0.5
483,691 - Pf6N2E2_415 0.90 -1.0
483,691 - Pf6N2E2_415 0.90 +1.5
483,859 + Pf6N2E2_416 0.16 -1.0
483,859 + Pf6N2E2_416 0.16 +0.8
484,239 + Pf6N2E2_416 0.87 +0.1
484,239 + Pf6N2E2_416 0.87 +1.4
484,240 - Pf6N2E2_416 0.87 -0.7
484,240 - Pf6N2E2_416 0.87 -0.2
484,240 - Pf6N2E2_416 0.87 -0.5
484,251 - Pf6N2E2_416 0.89 -0.3
484,251 - Pf6N2E2_416 0.89 +0.6
484,324 - +1.8
484,324 - -0.1
484,324 - +1.8
484,394 + +0.2
484,394 + +0.3
484,395 - +1.2
485,630 + Pf6N2E2_417 0.77 +0.8
485,631 - Pf6N2E2_417 0.77 -0.7
485,700 - Pf6N2E2_417 0.81 -1.2
485,810 + Pf6N2E2_417 0.88 -0.6
485,810 + Pf6N2E2_417 0.88 -0.4
485,811 - Pf6N2E2_417 0.88 -0.6
485,985 + -0.2
485,985 + -2.1
485,986 - -0.3
485,986 - +0.8
485,986 - -0.1
485,993 + +0.6
485,993 + -0.8
485,993 + +0.2
485,993 + -1.3
485,993 + -0.2
485,994 - +0.3
485,994 - +0.4
485,994 - -0.5
486,014 + -0.4
486,014 + +0.4
486,014 + -0.3
486,014 + +0.1
486,015 - +3.3
486,034 + -0.1
486,061 + -0.7
486,062 - +0.2
486,371 + Pf6N2E2_418 0.16 -0.5
486,371 + Pf6N2E2_418 0.16 -0.4
486,449 + Pf6N2E2_418 0.19 -0.1
486,449 + Pf6N2E2_418 0.19 -0.4
486,449 + Pf6N2E2_418 0.19 -0.2
486,449 + Pf6N2E2_418 0.19 +0.4
486,449 + Pf6N2E2_418 0.19 +0.6
486,449 + Pf6N2E2_418 0.19 -1.3
486,450 - Pf6N2E2_418 0.19 +1.2
486,450 - Pf6N2E2_418 0.19 -1.4
486,450 - Pf6N2E2_418 0.19 -0.7
486,450 - Pf6N2E2_418 0.19 +0.2
486,450 - Pf6N2E2_418 0.19 +0.0
486,450 - Pf6N2E2_418 0.19 +1.0
486,450 - Pf6N2E2_418 0.19 +0.1
486,668 + Pf6N2E2_418 0.30 +0.1
486,668 + Pf6N2E2_418 0.30 +1.2
486,668 + Pf6N2E2_418 0.30 +2.0
486,669 - Pf6N2E2_418 0.30 -0.5
486,669 - Pf6N2E2_418 0.30 -2.0

Or see this region's nucleotide sequence