Experiment: Vancomycin 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_406 and Pf6N2E2_407 are separated by 91 nucleotides Pf6N2E2_407 and Pf6N2E2_408 are separated by 468 nucleotides Pf6N2E2_408 and Pf6N2E2_409 are separated by 110 nucleotides
Pf6N2E2_406 - hypothetical protein, at 476,143 to 477,102
_406
Pf6N2E2_407 - Phosphohydrolase (MutT/nudix family protein), at 477,194 to 477,529
_407
Pf6N2E2_408 - Transcriptional regulators, LysR family, at 477,998 to 478,864
_408
Pf6N2E2_409 - Permease of the drug/metabolite transporter (DMT) superfamily, at 478,975 to 479,898
_409
Position (kb) 477
478
479 Strain fitness (log2 ratio) -2
-1
0
1
2 at 477.009 kb on - strand at 477.009 kb on - strand at 477.045 kb on + strand at 477.178 kb on + strand at 477.178 kb on + strand at 477.221 kb on + strand at 477.257 kb on + strand, within Pf6N2E2_407 at 477.301 kb on + strand, within Pf6N2E2_407 at 477.302 kb on - strand, within Pf6N2E2_407 at 477.437 kb on + strand, within Pf6N2E2_407 at 477.437 kb on + strand, within Pf6N2E2_407 at 477.437 kb on + strand, within Pf6N2E2_407 at 477.438 kb on - strand, within Pf6N2E2_407 at 477.438 kb on - strand, within Pf6N2E2_407 at 477.487 kb on - strand, within Pf6N2E2_407 at 477.532 kb on + strand at 477.606 kb on + strand at 477.606 kb on + strand at 477.606 kb on + strand at 477.606 kb on + strand at 477.606 kb on + strand at 477.606 kb on + strand at 477.606 kb on + strand at 477.658 kb on + strand at 477.658 kb on + strand at 477.658 kb on + strand at 477.658 kb on + strand at 477.658 kb on + strand at 477.658 kb on + strand at 477.658 kb on + strand at 477.658 kb on + strand at 477.658 kb on + strand at 477.658 kb on + strand at 477.658 kb on + strand at 477.658 kb on + strand at 477.658 kb on + strand at 477.659 kb on - strand at 477.659 kb on - strand at 477.659 kb on - strand at 477.659 kb on - strand at 477.659 kb on - strand at 477.659 kb on - strand at 477.659 kb on - strand at 477.659 kb on - strand at 477.662 kb on + strand at 477.662 kb on + strand at 477.662 kb on + strand at 477.663 kb on - strand at 477.750 kb on - strand at 477.750 kb on - strand at 477.903 kb on - strand at 477.927 kb on + strand at 477.927 kb on + strand at 477.927 kb on + strand at 477.927 kb on + strand at 477.927 kb on + strand at 477.927 kb on + strand at 477.927 kb on + strand at 477.928 kb on - strand at 477.928 kb on - strand at 477.928 kb on - strand at 477.928 kb on - strand at 477.928 kb on - strand at 477.928 kb on - strand at 477.928 kb on - strand at 477.928 kb on - strand at 477.928 kb on - strand at 477.995 kb on + strand at 477.995 kb on + strand at 477.995 kb on + strand at 477.996 kb on - strand at 477.996 kb on - strand at 477.996 kb on - strand at 477.996 kb on - strand at 477.996 kb on - strand at 477.999 kb on + strand at 477.999 kb on + strand at 478.000 kb on - strand at 478.029 kb on - strand at 478.074 kb on + strand at 478.074 kb on + strand at 478.074 kb on + strand at 478.113 kb on + strand, within Pf6N2E2_408 at 478.114 kb on - strand, within Pf6N2E2_408 at 478.149 kb on + strand, within Pf6N2E2_408 at 478.149 kb on + strand, within Pf6N2E2_408 at 478.150 kb on - strand, within Pf6N2E2_408 at 478.150 kb on - strand, within Pf6N2E2_408 at 478.213 kb on - strand, within Pf6N2E2_408 at 478.315 kb on + strand, within Pf6N2E2_408 at 478.316 kb on - strand, within Pf6N2E2_408 at 478.391 kb on + strand, within Pf6N2E2_408 at 478.392 kb on - strand, within Pf6N2E2_408 at 478.519 kb on - strand, within Pf6N2E2_408 at 478.530 kb on + strand, within Pf6N2E2_408 at 478.530 kb on + strand, within Pf6N2E2_408 at 478.530 kb on + strand, within Pf6N2E2_408 at 478.541 kb on + strand, within Pf6N2E2_408 at 478.541 kb on + strand, within Pf6N2E2_408 at 478.584 kb on - strand, within Pf6N2E2_408 at 478.897 kb on - strand at 478.907 kb on + strand at 478.907 kb on + strand at 478.908 kb on - strand at 478.929 kb on + strand at 478.930 kb on - strand at 478.939 kb on + strand at 479.030 kb on + strand at 479.074 kb on + strand, within Pf6N2E2_409 at 479.074 kb on + strand, within Pf6N2E2_409 at 479.075 kb on - strand, within Pf6N2E2_409 at 479.110 kb on + strand, within Pf6N2E2_409 at 479.110 kb on + strand, within Pf6N2E2_409 at 479.111 kb on - strand, within Pf6N2E2_409 at 479.186 kb on + strand, within Pf6N2E2_409 at 479.213 kb on + strand, within Pf6N2E2_409 at 479.272 kb on + strand, within Pf6N2E2_409 at 479.506 kb on + strand, within Pf6N2E2_409 at 479.507 kb on - strand, within Pf6N2E2_409 at 479.530 kb on - strand, within Pf6N2E2_409 at 479.539 kb on + strand, within Pf6N2E2_409 at 479.563 kb on + strand, within Pf6N2E2_409 at 479.563 kb on + strand, within Pf6N2E2_409 at 479.693 kb on - strand, within Pf6N2E2_409 at 479.707 kb on + strand, within Pf6N2E2_409 at 479.707 kb on + strand, within Pf6N2E2_409 at 479.707 kb on + strand, within Pf6N2E2_409 at 479.707 kb on + strand, within Pf6N2E2_409 at 479.707 kb on + strand, within Pf6N2E2_409 at 479.708 kb on - strand, within Pf6N2E2_409 at 479.708 kb on - strand, within Pf6N2E2_409 at 479.708 kb on - strand, within Pf6N2E2_409 at 479.752 kb on + strand, within Pf6N2E2_409
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.12 mg/ml remove 477,009 - -1.7 477,009 - -1.2 477,045 + -0.5 477,178 + +0.9 477,178 + +0.3 477,221 + +0.4 477,257 + Pf6N2E2_407 0.19 -0.1 477,301 + Pf6N2E2_407 0.32 -0.5 477,302 - Pf6N2E2_407 0.32 -1.5 477,437 + Pf6N2E2_407 0.72 -0.2 477,437 + Pf6N2E2_407 0.72 -1.1 477,437 + Pf6N2E2_407 0.72 +0.0 477,438 - Pf6N2E2_407 0.73 +0.2 477,438 - Pf6N2E2_407 0.73 -0.1 477,487 - Pf6N2E2_407 0.87 +0.1 477,532 + -0.4 477,606 + +1.0 477,606 + -0.2 477,606 + -0.0 477,606 + -0.1 477,606 + +0.6 477,606 + +1.0 477,606 + +1.0 477,658 + -1.7 477,658 + +0.9 477,658 + -0.0 477,658 + -1.0 477,658 + -0.2 477,658 + +0.5 477,658 + -1.0 477,658 + +0.2 477,658 + +0.8 477,658 + +0.6 477,658 + -0.2 477,658 + -0.1 477,658 + +0.3 477,659 - -1.8 477,659 - -1.1 477,659 - -0.7 477,659 - +2.0 477,659 - -0.0 477,659 - +0.2 477,659 - +0.1 477,659 - -1.4 477,662 + -0.5 477,662 + +0.1 477,662 + +0.6 477,663 - +0.5 477,750 - +0.4 477,750 - -0.0 477,903 - +0.2 477,927 + -0.5 477,927 + +0.2 477,927 + -1.7 477,927 + +0.9 477,927 + -1.2 477,927 + -0.1 477,927 + -0.1 477,928 - +0.2 477,928 - -0.8 477,928 - -0.8 477,928 - +0.2 477,928 - -0.2 477,928 - -0.7 477,928 - -0.2 477,928 - -1.0 477,928 - -0.5 477,995 + +0.2 477,995 + +0.4 477,995 + -0.2 477,996 - +0.1 477,996 - -0.4 477,996 - -0.7 477,996 - -0.1 477,996 - -0.3 477,999 + +0.0 477,999 + +0.1 478,000 - +0.7 478,029 - -1.3 478,074 + +0.7 478,074 + +0.2 478,074 + +0.5 478,113 + Pf6N2E2_408 0.13 -0.0 478,114 - Pf6N2E2_408 0.13 +0.3 478,149 + Pf6N2E2_408 0.17 +0.1 478,149 + Pf6N2E2_408 0.17 -1.4 478,150 - Pf6N2E2_408 0.18 -1.0 478,150 - Pf6N2E2_408 0.18 -1.1 478,213 - Pf6N2E2_408 0.25 -0.3 478,315 + Pf6N2E2_408 0.37 -0.6 478,316 - Pf6N2E2_408 0.37 -0.3 478,391 + Pf6N2E2_408 0.45 +1.9 478,392 - Pf6N2E2_408 0.45 -0.4 478,519 - Pf6N2E2_408 0.60 -0.1 478,530 + Pf6N2E2_408 0.61 -0.8 478,530 + Pf6N2E2_408 0.61 +0.9 478,530 + Pf6N2E2_408 0.61 -1.3 478,541 + Pf6N2E2_408 0.63 +0.4 478,541 + Pf6N2E2_408 0.63 -0.3 478,584 - Pf6N2E2_408 0.68 +0.8 478,897 - -1.1 478,907 + -0.2 478,907 + -1.0 478,908 - -0.7 478,929 + -0.1 478,930 - +0.3 478,939 + -0.0 479,030 + +0.0 479,074 + Pf6N2E2_409 0.11 +1.0 479,074 + Pf6N2E2_409 0.11 -0.5 479,075 - Pf6N2E2_409 0.11 +0.2 479,110 + Pf6N2E2_409 0.15 -1.3 479,110 + Pf6N2E2_409 0.15 +0.2 479,111 - Pf6N2E2_409 0.15 -0.2 479,186 + Pf6N2E2_409 0.23 +0.4 479,213 + Pf6N2E2_409 0.26 +0.3 479,272 + Pf6N2E2_409 0.32 +0.3 479,506 + Pf6N2E2_409 0.57 -0.5 479,507 - Pf6N2E2_409 0.58 -0.3 479,530 - Pf6N2E2_409 0.60 +1.0 479,539 + Pf6N2E2_409 0.61 +0.5 479,563 + Pf6N2E2_409 0.64 +0.2 479,563 + Pf6N2E2_409 0.64 -0.2 479,693 - Pf6N2E2_409 0.78 +0.3 479,707 + Pf6N2E2_409 0.79 +0.1 479,707 + Pf6N2E2_409 0.79 -0.3 479,707 + Pf6N2E2_409 0.79 +0.3 479,707 + Pf6N2E2_409 0.79 +1.2 479,707 + Pf6N2E2_409 0.79 -0.1 479,708 - Pf6N2E2_409 0.79 +1.2 479,708 - Pf6N2E2_409 0.79 -0.9 479,708 - Pf6N2E2_409 0.79 +0.3 479,752 + Pf6N2E2_409 0.84 +0.0
Or see this region's nucleotide sequence