Experiment: Vancomycin 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3010 and Pf6N2E2_3011 overlap by 1 nucleotides Pf6N2E2_3011 and Pf6N2E2_3012 overlap by 4 nucleotides Pf6N2E2_3012 and Pf6N2E2_3013 are separated by 124 nucleotides Pf6N2E2_3013 and Pf6N2E2_3014 are separated by 9 nucleotides Pf6N2E2_3014 and Pf6N2E2_3015 overlap by 8 nucleotides Pf6N2E2_3015 and Pf6N2E2_3016 overlap by 1 nucleotides
Pf6N2E2_3010 - Bacteriophage tail sheath protein, at 3,498,726 to 3,500,222
_3010
Pf6N2E2_3011 - Mu-like prophage FluMu protein GP38, at 3,500,222 to 3,500,404
_3011
Pf6N2E2_3012 - FIG111678: IS, phage, Tn; Transposon-related functions, at 3,500,401 to 3,500,985
_3012
Pf6N2E2_3013 - FIG032563: Phage tail protein D, at 3,501,110 to 3,502,126
_3013
Pf6N2E2_3014 - P2-like prophage tail protein X, at 3,502,136 to 3,502,348
_3014
Pf6N2E2_3015 - hypothetical protein, at 3,502,341 to 3,502,724
_3015
Pf6N2E2_3016 - Phage tail length tape-measure protein, at 3,502,724 to 3,504,901
_3016
Position (kb)
3501
3502
3503 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3500.142 kb on - strand at 3500.223 kb on + strand at 3500.270 kb on - strand, within Pf6N2E2_3011 at 3500.411 kb on - strand at 3500.628 kb on + strand, within Pf6N2E2_3012 at 3500.628 kb on + strand, within Pf6N2E2_3012 at 3500.629 kb on - strand, within Pf6N2E2_3012 at 3500.657 kb on + strand, within Pf6N2E2_3012 at 3500.861 kb on + strand, within Pf6N2E2_3012 at 3500.861 kb on + strand, within Pf6N2E2_3012 at 3500.861 kb on + strand, within Pf6N2E2_3012 at 3500.861 kb on + strand, within Pf6N2E2_3012 at 3500.862 kb on - strand, within Pf6N2E2_3012 at 3500.862 kb on - strand, within Pf6N2E2_3012 at 3500.862 kb on - strand, within Pf6N2E2_3012 at 3500.862 kb on - strand, within Pf6N2E2_3012 at 3501.154 kb on - strand at 3501.240 kb on - strand, within Pf6N2E2_3013 at 3501.456 kb on + strand, within Pf6N2E2_3013 at 3501.456 kb on + strand, within Pf6N2E2_3013 at 3501.539 kb on + strand, within Pf6N2E2_3013 at 3501.539 kb on + strand, within Pf6N2E2_3013 at 3501.540 kb on - strand, within Pf6N2E2_3013 at 3501.597 kb on + strand, within Pf6N2E2_3013 at 3501.597 kb on + strand, within Pf6N2E2_3013 at 3501.597 kb on + strand, within Pf6N2E2_3013 at 3501.597 kb on + strand, within Pf6N2E2_3013 at 3501.597 kb on + strand, within Pf6N2E2_3013 at 3501.598 kb on - strand, within Pf6N2E2_3013 at 3501.598 kb on - strand, within Pf6N2E2_3013 at 3501.598 kb on - strand, within Pf6N2E2_3013 at 3501.598 kb on - strand, within Pf6N2E2_3013 at 3501.598 kb on - strand, within Pf6N2E2_3013 at 3501.598 kb on - strand, within Pf6N2E2_3013 at 3501.630 kb on + strand, within Pf6N2E2_3013 at 3501.631 kb on - strand, within Pf6N2E2_3013 at 3501.631 kb on - strand, within Pf6N2E2_3013 at 3502.217 kb on + strand, within Pf6N2E2_3014 at 3502.217 kb on + strand, within Pf6N2E2_3014 at 3502.299 kb on + strand, within Pf6N2E2_3014 at 3502.543 kb on + strand, within Pf6N2E2_3015 at 3502.544 kb on - strand, within Pf6N2E2_3015 at 3502.544 kb on - strand, within Pf6N2E2_3015 at 3502.544 kb on - strand, within Pf6N2E2_3015 at 3502.729 kb on + strand at 3502.729 kb on + strand at 3502.730 kb on - strand at 3502.730 kb on - strand at 3502.866 kb on + strand at 3502.866 kb on + strand at 3502.867 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.12 mg/ml remove 3,500,142 - -0.0 3,500,223 + -0.4 3,500,270 - Pf6N2E2_3011 0.26 +0.2 3,500,411 - +3.2 3,500,628 + Pf6N2E2_3012 0.39 +0.1 3,500,628 + Pf6N2E2_3012 0.39 +0.2 3,500,629 - Pf6N2E2_3012 0.39 +0.5 3,500,657 + Pf6N2E2_3012 0.44 +0.0 3,500,861 + Pf6N2E2_3012 0.79 +2.0 3,500,861 + Pf6N2E2_3012 0.79 -0.1 3,500,861 + Pf6N2E2_3012 0.79 -0.9 3,500,861 + Pf6N2E2_3012 0.79 +0.3 3,500,862 - Pf6N2E2_3012 0.79 +0.1 3,500,862 - Pf6N2E2_3012 0.79 +0.3 3,500,862 - Pf6N2E2_3012 0.79 +0.5 3,500,862 - Pf6N2E2_3012 0.79 +0.1 3,501,154 - +1.3 3,501,240 - Pf6N2E2_3013 0.13 -0.6 3,501,456 + Pf6N2E2_3013 0.34 -0.1 3,501,456 + Pf6N2E2_3013 0.34 +0.9 3,501,539 + Pf6N2E2_3013 0.42 +0.8 3,501,539 + Pf6N2E2_3013 0.42 -0.0 3,501,540 - Pf6N2E2_3013 0.42 +0.0 3,501,597 + Pf6N2E2_3013 0.48 -0.6 3,501,597 + Pf6N2E2_3013 0.48 -0.7 3,501,597 + Pf6N2E2_3013 0.48 +0.5 3,501,597 + Pf6N2E2_3013 0.48 -2.0 3,501,597 + Pf6N2E2_3013 0.48 -1.0 3,501,598 - Pf6N2E2_3013 0.48 -0.4 3,501,598 - Pf6N2E2_3013 0.48 +0.1 3,501,598 - Pf6N2E2_3013 0.48 -0.9 3,501,598 - Pf6N2E2_3013 0.48 -0.4 3,501,598 - Pf6N2E2_3013 0.48 -0.4 3,501,598 - Pf6N2E2_3013 0.48 -0.2 3,501,630 + Pf6N2E2_3013 0.51 -0.4 3,501,631 - Pf6N2E2_3013 0.51 +0.4 3,501,631 - Pf6N2E2_3013 0.51 -0.1 3,502,217 + Pf6N2E2_3014 0.38 +0.7 3,502,217 + Pf6N2E2_3014 0.38 +0.5 3,502,299 + Pf6N2E2_3014 0.77 +0.2 3,502,543 + Pf6N2E2_3015 0.53 +0.0 3,502,544 - Pf6N2E2_3015 0.53 -0.4 3,502,544 - Pf6N2E2_3015 0.53 -0.2 3,502,544 - Pf6N2E2_3015 0.53 -1.2 3,502,729 + +0.9 3,502,729 + +0.0 3,502,730 - -0.9 3,502,730 - -0.4 3,502,866 + -0.5 3,502,866 + +0.5 3,502,867 - -0.3
Or see this region's nucleotide sequence