Experiment: Vancomycin 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2940 and Pf6N2E2_2941 are separated by 116 nucleotides Pf6N2E2_2941 and Pf6N2E2_2942 are separated by 271 nucleotides
Pf6N2E2_2940 - OmpA family protein, at 3,431,649 to 3,432,761
_2940
Pf6N2E2_2941 - Esterase A, at 3,432,878 to 3,434,023
_2941
Pf6N2E2_2942 - ATP-dependent helicase DinG/Rad3, at 3,434,295 to 3,436,439
_2942
Position (kb)
3432
3433
3434
3435 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3431.902 kb on + strand, within Pf6N2E2_2940 at 3431.902 kb on + strand, within Pf6N2E2_2940 at 3431.902 kb on + strand, within Pf6N2E2_2940 at 3431.902 kb on + strand, within Pf6N2E2_2940 at 3431.902 kb on + strand, within Pf6N2E2_2940 at 3431.902 kb on + strand, within Pf6N2E2_2940 at 3431.903 kb on - strand, within Pf6N2E2_2940 at 3431.903 kb on - strand, within Pf6N2E2_2940 at 3431.903 kb on - strand, within Pf6N2E2_2940 at 3431.903 kb on - strand, within Pf6N2E2_2940 at 3432.341 kb on - strand, within Pf6N2E2_2940 at 3432.369 kb on - strand, within Pf6N2E2_2940 at 3432.750 kb on - strand at 3432.782 kb on + strand at 3432.782 kb on + strand at 3432.782 kb on + strand at 3432.783 kb on - strand at 3432.783 kb on - strand at 3432.783 kb on - strand at 3432.783 kb on - strand at 3432.794 kb on - strand at 3432.794 kb on - strand at 3432.879 kb on + strand at 3432.880 kb on - strand at 3432.881 kb on + strand at 3432.881 kb on + strand at 3432.881 kb on + strand at 3432.881 kb on + strand at 3432.882 kb on - strand at 3432.882 kb on - strand at 3432.882 kb on - strand at 3432.932 kb on + strand at 3432.932 kb on + strand at 3432.932 kb on + strand at 3432.932 kb on + strand at 3432.932 kb on + strand at 3432.932 kb on + strand at 3432.933 kb on - strand at 3432.933 kb on - strand at 3432.933 kb on - strand at 3432.933 kb on - strand at 3432.933 kb on - strand at 3432.933 kb on - strand at 3432.933 kb on - strand at 3432.933 kb on - strand at 3432.933 kb on - strand at 3432.936 kb on + strand at 3432.936 kb on + strand at 3432.937 kb on - strand at 3432.937 kb on - strand at 3432.937 kb on - strand at 3432.937 kb on - strand at 3433.048 kb on - strand, within Pf6N2E2_2941 at 3433.048 kb on - strand, within Pf6N2E2_2941 at 3433.062 kb on - strand, within Pf6N2E2_2941 at 3433.200 kb on + strand, within Pf6N2E2_2941 at 3433.542 kb on + strand, within Pf6N2E2_2941 at 3433.543 kb on - strand, within Pf6N2E2_2941 at 3433.557 kb on + strand, within Pf6N2E2_2941 at 3433.558 kb on - strand, within Pf6N2E2_2941 at 3433.629 kb on + strand, within Pf6N2E2_2941 at 3433.629 kb on + strand, within Pf6N2E2_2941 at 3433.629 kb on + strand, within Pf6N2E2_2941 at 3433.629 kb on + strand, within Pf6N2E2_2941 at 3434.004 kb on + strand at 3434.004 kb on + strand at 3434.573 kb on - strand, within Pf6N2E2_2942 at 3435.010 kb on + strand, within Pf6N2E2_2942
Per-strain Table
Position Strand Gene LocusTag Fraction Vancomycin 0.12 mg/ml remove 3,431,902 + Pf6N2E2_2940 0.23 -1.5 3,431,902 + Pf6N2E2_2940 0.23 -1.6 3,431,902 + Pf6N2E2_2940 0.23 -1.8 3,431,902 + Pf6N2E2_2940 0.23 -2.9 3,431,902 + Pf6N2E2_2940 0.23 -1.2 3,431,902 + Pf6N2E2_2940 0.23 -2.2 3,431,903 - Pf6N2E2_2940 0.23 -0.8 3,431,903 - Pf6N2E2_2940 0.23 -1.0 3,431,903 - Pf6N2E2_2940 0.23 -1.7 3,431,903 - Pf6N2E2_2940 0.23 -1.6 3,432,341 - Pf6N2E2_2940 0.62 -1.3 3,432,369 - Pf6N2E2_2940 0.65 -1.7 3,432,750 - -1.3 3,432,782 + -0.6 3,432,782 + -1.3 3,432,782 + -2.6 3,432,783 - -0.4 3,432,783 - -0.5 3,432,783 - -1.6 3,432,783 - +1.0 3,432,794 - +0.4 3,432,794 - -0.2 3,432,879 + -0.7 3,432,880 - +1.9 3,432,881 + -2.3 3,432,881 + -0.9 3,432,881 + -2.0 3,432,881 + -2.7 3,432,882 - +0.1 3,432,882 - -0.2 3,432,882 - -0.2 3,432,932 + -0.4 3,432,932 + -1.0 3,432,932 + -1.8 3,432,932 + -0.0 3,432,932 + -0.8 3,432,932 + -1.3 3,432,933 - +0.6 3,432,933 - -0.9 3,432,933 - -0.3 3,432,933 - +0.8 3,432,933 - +0.1 3,432,933 - -0.0 3,432,933 - +0.0 3,432,933 - -1.8 3,432,933 - -0.1 3,432,936 + -1.3 3,432,936 + -1.7 3,432,937 - +0.6 3,432,937 - -0.4 3,432,937 - -0.7 3,432,937 - +0.2 3,433,048 - Pf6N2E2_2941 0.15 -0.3 3,433,048 - Pf6N2E2_2941 0.15 +0.4 3,433,062 - Pf6N2E2_2941 0.16 -0.5 3,433,200 + Pf6N2E2_2941 0.28 -0.8 3,433,542 + Pf6N2E2_2941 0.58 -2.0 3,433,543 - Pf6N2E2_2941 0.58 -0.9 3,433,557 + Pf6N2E2_2941 0.59 -0.3 3,433,558 - Pf6N2E2_2941 0.59 +2.7 3,433,629 + Pf6N2E2_2941 0.66 -1.2 3,433,629 + Pf6N2E2_2941 0.66 -1.5 3,433,629 + Pf6N2E2_2941 0.66 -0.6 3,433,629 + Pf6N2E2_2941 0.66 -1.6 3,434,004 + -0.5 3,434,004 + -0.1 3,434,573 - Pf6N2E2_2942 0.13 -0.3 3,435,010 + Pf6N2E2_2942 0.33 -0.8
Or see this region's nucleotide sequence